LOCUS       sequence-28946-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Zhu G, Wu CJ, Zhao Y, Ashwell JD
  TITLE     Optineurin negatively regulates TNFalpha- induced NF-kappaB 
            activation by competing with NEMO for ubiquitinated RIP.
  JOURNAL   Curr Biol. 2007 Aug 21. 17(16):1438-43.
  PUBMED    17702576
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2007 Aug 21. 17(16):1438-43."
COMMENT     Sequence Label: pDEST26-hOPTN-D474N
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     405..427
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     419..436
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     420..436
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     447..466
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        447..465
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    498..522
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctgcataac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
       61 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      121 catgaacaga ctgtgaggac tgaggggcct gaaatgagcc ttgggactgt gaatctaaaa
      181 tacacaaaca attagaatca ctagctcctg tgtataatat tttcataaat catactcagt
      241 aagcaaaact ctcaagcagc aagcatatgc agctagttta acacattata cacttaaaaa
      301 ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc
      361 acagaagtaa ggttccttca caaagatccc aagctagcag ttttcccagt cacgacgttg
      421 taaaacgacg gccagtgcct agcttataat acgactcact atagggagag agctatgacg
      481 tcgcatgcac gcgatcaacc actttgtaca agaaagctgg gtcggcgcgc ccaccctttt
      541 aaatgatgca atccatcacg tgaatctgta acgtgtctat gtcaggcaga acctctccac
      601 acttggggca ggaatgaatc ggaatattcc gctgttgccg ccagtccctg tcctcagctc
      661 ctctttgaac aaggtaagcc tgctggtcag agtcacttgt tctcgcccca tgacgactct
      721 gcatctccat caaggactgc ctgcctccgt cttcgaaagc atcatctc
//
