LOCUS       sequence-28947-e         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Zhu G, Wu CJ, Zhao Y, Ashwell JD
  TITLE     Optineurin negatively regulates TNFalpha- induced NF-kappaB 
            activation by competing with NEMO for ubiquitinated RIP.
  JOURNAL   Curr Biol. 2007 Aug 21. 17(16):1438-43.
  PUBMED    17702576
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2007 Aug 21. 17(16):1438-43."
COMMENT     Sequence Label: pDEST26-hOPTN-D474N
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             137..154
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    164..188
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             523..534
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggggnntta gcgagctgtt tgtgaaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgggaccga tccagcctcc ggactctagc ctaggccgcg
      121 gaccatggcg tactaccatc accatcacca tcactctaga tcaacaagtt tgtacaaaaa
      181 agcaggctcc gcggccgccc ccttcacctc ccatcaacct ctcagctgcc tcactgaaaa
      241 ggaggacagc cccagtgaaa gcacaggaaa tggacccccc cacctggccc acccaaacct
      301 ggacacgttt accccggagg agctgctgca gcagatgaaa gagctcctga ccgagaacca
      361 ccagctgaaa gaagccatga agctaaataa tcaagccatg aaagggagat ttgaggagct
      421 ttcggcctgg acagagaaac agaaggaaga acgccagttt tttgagatac agagcaaaga
      481 agcaaaagag cgtctaatgg ccttgagtca tgagaatgag aaattgaagg aagagcttgg
      541 aaaactaaaa gggaaatcag aaaggtcatc tgaggacccc actgatgact ccaggcttcc
      601 cagggccgaa gcggagcagg aaaaggacca gctcaggacc cngtggnggg ggggttcaan
      661 aaaaaaatgg ntttgttggt tttttnaat
//
