LOCUS       sequence-289476-       13575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13575)
  AUTHORS   Igreja C, Izaurralde E
  TITLE     CUP promotes deadenylation and inhibits decapping of mRNA targets.
  JOURNAL   Genes Dev. 2011 Sep 15;25(18):1955-67. doi: 10.1101/gad.17136311.
  PUBMED    21937713
REFERENCE   2  (bases 1 to 13575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2011-09-15- 15"; volume: "25"; 
            issue: "18"; pages: "1955-67"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1-dsRNAres_N
FEATURES             Location/Qualifiers
     source          1..13575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10860..10901
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10911..10928
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10951..10968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11001..11135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11025..11044)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11079..11098
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11757..12345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11837..11856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12516..13307,13308..13376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13139..13158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13377..13481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13549..13567
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttaattaaga tgaacgtaga gagccaactg aagacaccgc caacgcacat
     3361 tcgaaatctg gtcaagcacg tgaacaagcg aaattttaat gagagcagcg agcaaataaa
     3421 tcagttcgtt aaagagcacg gcctggaagc ggatcgcagc agcctgcgcc acctgttctc
     3481 cgtgatcaac ttcagcgatc tcgtgccatc agttacggtc cagctgcagg cgaagctcct
     3541 gggcatccag ttggagcgtc agcttcacag ttcatcgttt gtgtccaaca tctgctacgc
     3601 ttttgaccag ttcttcgcca gcaaccagaa gtccctgaag cctgttgcgg tagcggatct
     3661 cgttggccag gtcgcccgac tgaccggcat caacaaggtt tgtgagtgcg tctttgcctt
     3721 ggccttgacc cactcctcgt acacagaact caaacattct gccaggaaca acctaaaagg
     3781 tagcctcagc gaacttatag actcttactt gggtaataaa ggcaccggcc cagccgacag
     3841 cggtctccgg gagatctcct tcgatctact gcagtatttg ctgtgctgcc ttagcgagta
     3901 cgtacaaccg caggtggaag cacagttttt gatcaagctt cgtgaggagt ttccgcgcca
     3961 ggcggtaccg ctggttctag cgccgttcct ttacggctcg acgacggcga cgattgcggg
     4021 agcaggtgcc agcgaaacgg atgcggaggc cgaagcgacg actaatagca acagctccag
     4081 tttcgaagca gatgctttga acgaggtggg aatcgaggat atttatgacc atttaagcga
     4141 gataatattc accaaccagg gcaaaaataa tattatggac acatcttgga ttaatctaat
     4201 ccttgaaatc ggttatgaat ttacatcgag cgttgaagag tgtaagaatc atttgtgttc
     4261 ccgcgagcga gaacgcgctg agctccagtc caaagatgtc gccaagatcg tgggtcttat
     4321 gtgccgacgg cactcctccc tgctcgattg taatgtgaat ctaccgacgc cggcgaactt
     4381 ctggcctggt cagggacagg gcggtggcag caacagcagt ggctcttcgc agacacaaat
     4441 cactccgcag caacagaacc cgggcagcag taacaacaac gatggcagcg atggcaattc
     4501 gtccagtgat aagaaggaca agaaggagac gacggaggcc acgcaaactt ggaagccaga
     4561 tgtctttgtg caggcgctca aggaggtggt gcctcagctc aactggaagg acgtgtgcat
     4621 ggaacttgat catcccgagt tcgtgctgaa ggatcgcatc ggtttggagc ttttgctcac
     4681 catccttcgg ctggccactg gctctaacat atttccacat ccagaatgca tttaccgtca
     4741 ctgggcaaac acggaaggtc agctgtcgct tatcgcgaca atgctgaaga acccggatct
     4801 cttttccttc gccgactttg tatttagcca gccagcgttg gatgtgctta agacagctcc
     4861 ggatgcggac aataaggaga tctcgtcctg gaagtcgctt cacctggtgg aggtgctgct
     4921 ctccattgcg gacaagggat actatacgca ggtccatgag ctattcaagt tcccagcgca
     4981 gaactgtccc gatgtgctat ttttggcctt attgaacaca agtccgccta tgacgccact
     5041 gcgtcaggat ctgtttaacc aactgatacc aacgttcctt ggcaaccatc ccaactcgaa
     5101 cgtgatcttg gccagcgcct ggagctcgaa cagcttccaa cttcgttcca atatcatgaa
     5161 tgctatgtcc gagtggtatc tccgaggcaa tgagttcgat caggtgaaac tgtcgcgcat
     5221 cctggatctt gcccaggatt taaaagcgct atcagcgctg ctcaacgccc gttcattttt
     5281 attcattatc gacctggctt gccttgcctc tcggcgcgag tacctgaagc tggaaaagtg
     5341 gcttaccgat aaaattcgcg aacatggaga gccttttatg caggctataa tcaaggttct
     5401 gcaccgccgc tgcccgcagg ttatcaacgc caaagtgcca gaggatcagc tgcctcccaa
     5461 gcaggcgcag cttttgcccg agacagttac aaccatgatc aactgtctgc aaacgtgcat
     5521 caataactgc atgcagccgg agatggtcga agtcatcatg caaatgaccg cgaacgtggc
     5581 gattatggct aacatggcgc gtgcccaaca gcagcagcag ccaggattgg ttccgccgcc
     5641 tccaccaaca attctgcgcg gccatcgtgg tatggatctg cctggaggca tagtccctcc
     5701 gccaccgcag caaccgttct ccggaaacct caacgcgcag atgtttggac caggaatgga
     5761 tccgctaacg aatatgtcca acaacttagc cggcctcaac ctcagcggcc cgaacggagc
     5821 atttaatttt ggaaacatgc taacttctcc atcgcgtctg atgactccgg gagccaatcc
     5881 ctatccgctg aacctcatgc agatgccgca ggctccgcca ccacccaacg taggaaacct
     5941 aggccgcatg ctgccagggg gtccacaaca gcagactcca acgccgactc caacggcccc
     6001 caatccaaac aaccctgtga tggccgatct ccagatacct gtgtcaaagg aagttgaaga
     6061 cgaggtcaac tcatactttc agcgcatcta caaccaccag ccgaatccaa cgctgtccat
     6121 cgatgaagtg ctggacattt tgcaaagatt taaagagtca agtaaccggc gcgagcagga
     6181 agtctttttg tgcatgctgc gcaatctgtt cgaggaatat cgctttttct gtcaataccc
     6241 agagaaagag ctacagatta ctgcgcagct atttggtggc attattgatc gtaacctggt
     6301 gcccactttt gttgcactgg gcctttctct gcgctgtgtc ttggatgccc tccgcaagcc
     6361 cgatggatct aagctttact atttcggtgt gacggcactg gacagattca gaactcgatt
     6421 gcacacctac aacaaatact gcgagcatat ccgctccatt cctcacttct ctgacttccc
     6481 gccgcacctt attcagtatg tggaatacgg aatgcacggc caggagccgc cgccgcagaa
     6541 gttgattgga ctcagcaata cgattccttc ggcgatatct agcggaccag gaacggaacc
     6601 aatttacagg aatagctcca tgttaggtaa tatgcccgct gccaccccgg gatcgggacc
     6661 gaaatcaaac gctgctgtat cgcatgccac gagaatgaag tccatcgcca atgccaccaa
     6721 tatcgacaca cttttggtgg ccaaccagga agagaaagtg actgtgccac cagagcctgt
     6781 gcaggacaaa actgccttta ttttcaacaa cttgagccag ctgaacatac cgcagaagtg
     6841 cgatgagata aaggagatta tgaccaagga gtattggccg tggctagcgc aatatctggt
     6901 cctcaaacgc gcatcaatgg agttcaactt ccacacacta tattacaact ttttggatgc
     6961 ccttaaaaac ggcgagatca accgattcgt aaccaaagag accctacgca acatcaaggt
     7021 actcttgcgc tccgataagg gagttattaa tttctccgat cgaagtctgt tgaagaacct
     7081 cggacactgg ttgggcatga tgaccttagg tcgcaatcgc cccatcctgc agttggatct
     7141 ggatcttaaa tcccttttgg ctgaggctta ccacaagggc cagcaggaac tgctttttgt
     7201 ggttcctttc gtagccaaga tccttgagtc atccgccaag tctcgcatct tccgatcgcc
     7261 caatccatgg acaatgggta tcatgtacgt gcttgctgag cttcaccagg agccagacct
     7321 taagctgaac ctgaagtttg aaatcgaagt actctgcaaa acacttaatc tggagttggc
     7381 caagttgcga caagtgatct acctgaagga tcccaatcga actcaccgga ttgaagagca
     7441 gatgtcgcaa ccaaaaccga aacaacttga gccagtggct tctgcgccag ctctaccgcg
     7501 tgaacagcaa tccccggcac aacctccgcc accaccacaa cagcagcaac caccgcagca
     7561 gcaggtacct ccaccgccat cctctgcgga cgtggacgcc caaaatgcgg ccgctatgat
     7621 gatggcagcg ggcggagcta atagtactcc tggatcggta tcttcgccca atctacccac
     7681 cgattccagc caggtggcac taccgccacc agagccgcgc tattcatacg tggacgtaaa
     7741 cgtgagcaac ttccagctta ttggtcagca gttggtactg ccacccaata ctccgttcct
     7801 gcacgccaat cccggaatca aacacatggt cgtcaacgcc gtggagcgca cgataaccga
     7861 ctggttgcag cctattgtgg accgaagcat ccgcattgcc tgtgccacca ccgagcagat
     7921 catccgcaag gacttcgccc tggatgctga cgagaaccga atgcgcactg ccgctcatca
     7981 gatggtacga aaccttgccg ccggcatggc catgattacc ggtaaggatg agatagcgcg
     8041 tgctattagc cagaatctgc acaaggcctt gctgtcgggt ctaaatggga tgcccagcat
     8101 ggctgagatc caagctgctg ctatgcagtt agccagcgaa aatgttgagc tagtttgcgc
     8161 tttcattcag aaaacatctg ctgaaaaggc agccgcagag atcgataggc gtctaagcac
     8221 cgatttcgag accagaaaaa ttgctcgtga agagggcaac cgctttgtgg acgcccagat
     8281 cctcacctat caacaggagc gtcttccaga agcagtgcgc atcaaggtgg gcgcggctcc
     8341 agctacactt tatgctgtgt actcggagtt cgctagaagc atacctggct tccagcagat
     8401 gagcgatcgc gatattgccc tgtttgtgcc caagccgacg gatttgtctc agccaaatgt
     8461 gtttgctaat gatgatagca tggtatacgg ggaactggct agcaaaatgg aggccttcat
     8521 gaacacggca atcggagtgc cgacactcca gatccaggct agcaagatgc acatgctact
     8581 caacgccctg atagcgacgc gtcgactgcg tgaccaagag tctgccttta atctgctgac
     8641 ccgcgcggtc gagggtttga ccgaaggatt ggtcaatatg catgaaaaca tggagcaaat
     8701 gaagatgtac caaaacatcc atctccgtat ccttggcttg ctgaacaaca gtttcggtgc
     8761 tccaaacaca gagcgagcgg taacaaagtg tttcttcgat atccgagagg aggtgcgata
     8821 taacgtggag gcagctcgcg ctttgattac gtcgcacttt gtcaatctga atcagtttga
     8881 cggaatgcta cgcgactgca tggacaacgg aaacaattat gtggctatat cgttcggtat
     8941 cgcactgctg gagcgcctca tcatggacga tcgagtgatt aatattgttt cggacaacga
     9001 attcatggca actgttgagc tgctaggtag gctgacccaa caccgtcacc gctaccccga
     9061 gtgtatcgtc aacgccatcg acaccctgtg gtccggcaat ttcaatagct cctccgatta
     9121 ttcccccttt aatggaaacg accgctatct gagcggcgct agccactata ttcatagcgg
     9181 aatgcaccat gtccgctcgt gtgacaccga cgaccccccc ggactccagg aaaaaaccga
     9241 attcttgttg aaagactggg tggccttgta tacccagcaa aatcaacaat cgacccgtga
     9301 cgcccgtaat tttggcgcct tcgtgcaaaa aatgaatacc tacggcattt tgaagaccga
     9361 tgatctgatt acgcgcttct ttcgtcaagc tacccatatc tgtaccgacg tcgtctatcg
     9421 tatgtttgct gaacccagcc ttcccattaa tcaagctaag aataaaattt ttcaatggat
     9481 tgacgccttt gtgcacttga tcgccatgct ggtccgccat tcgggagagg ccggcaaccc
     9541 cacgacgaaa attaatttgc tcaataaagt cttgggaatc gtcttgggca ccttgatcaa
     9601 agatcacgaa atgcgcggag tgtcctttca acaagtggga tatcaccgtt tttttatgat
     9661 gttgtttatg gaattgtgta ccgctgacgt catcctggaa agcttgatgc atagcatcgt
     9721 cagcgctttt gcttacacat atcacttgtt gaatcccagc gtggctccgg gattctgctt
     9781 tgcttggttg gagctcattt cgcatcgcgt cttcctgggc cgcatcctgg tccaaattcc
     9841 cgggcaaaag ggctggccac tttacgctca attgctgcag gaccttttta aatatcttgc
     9901 gcccttctta cgcaacactg agctcggcaa accagtccag cttttgtaca aggggacgct
     9961 acgtgttctg ctcgttctgc ttcacgactt cccggagttc ttgtgcgact atcattttgg
    10021 gttctgtgac accatcccgc ccaattgcgt ccagatgcgt aacatcatcc tgtcggcgtt
    10081 tccgcgcaac atgcgcttgc ccgatccctt cacgcccaac ctaaaagtgg acatgctgtc
    10141 ggacagtagt aatgcgccta aggtgctcag tagctacatt atgaacatcc agcccgcgaa
    10201 ctttaagaag gatttggact cgtacctcaa ggcccgggcg ccagttactt ttctatcgga
    10261 attacgtggt cacttgcagg tgacaagcga gccaggaact cgttacaaca tggcgctgat
    10321 gaatgctctg gtcatgtatg tgggaactca ggcgattgct ttgatcagaa acaaaaactt
    10381 tgtgcccaac acctcgaaca tagcacacag cgcccacatg gatatcttcc agaatcttgc
    10441 tgttgacttg gatacggagg gtcgctatct gttcttgaat gcgattgcga accagttgcg
    10501 ctatcccaac agccacacgc actacttcag ctgtgcggtg ctgcatctgt ttgccgaggc
    10561 caactcggag gcaattcagg agcagatcac tcgcgtactt ctggaacgat tgatcgtgaa
    10621 ccgcccgcat ccttggggac tgttgatcac ttttattgag ctgatcaaaa atcccatcta
    10681 caagttctgg gaccatgact ttgtgcactg tgcgccagag atcaccaagc tctttgaatc
    10741 ggtggcccgt tcctgtctgg ccaagtcgaa cgtcacccag cagttgaaca tgcccgtcgt
    10801 cgatggcgag ggccaggagg tagccaccat caactgatct agagggcccg cggttcgaag
    10861 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
    10921 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc
    10981 gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa
    11041 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
    11101 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
    11161 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
    11221 gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc
    11281 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
    11341 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
    11401 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
    11461 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
    11521 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
    11581 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
    11641 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
    11701 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    11761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11941 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    12181 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    12241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    12301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    12361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    12421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    12481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    12541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    12601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    12661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    12721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    12781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
    12841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    12901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    12961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    13021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    13081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
    13141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    13201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    13261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    13321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    13381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    13441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    13501 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    13561 acgaggccct ttcgt
//
