LOCUS       sequence-28948-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Zhu G, Wu CJ, Zhao Y, Ashwell JD
  TITLE     Optineurin negatively regulates TNFalpha- induced NF-kappaB 
            activation by competing with NEMO for ubiquitinated RIP.
  JOURNAL   Curr Biol. 2007 Aug 21. 17(16):1438-43.
  PUBMED    17702576
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2007 Aug 21. 17(16):1438-43."
COMMENT     Sequence Label: pDEST17-hOPTN
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             428..439
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tttgtttact ttaagaagga gatatacata tgtcgtacta ccatcaccat caccatcacc
       61 tcgaatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc acctcccatc
      121 aacctctcag ctgcctcact gaaaaggagg acagccccag tgaaagcaca ggaaatggac
      181 ccccccacct ggcccaccca aacctggaca cgtttacccc ggaggagctg ctgcagcaga
      241 tgaaagagct cctgaccgag aaccaccagc tgaaagaagc catgaagcta aataatcaag
      301 ccatgaaagg gagatttgag gagctttcgg cctggacaga gaaacagaag gaagaacgcc
      361 agttttttga gatacagagc aaagaagcaa aagagcgtct aatggccttg agtcatgaga
      421 atgagaaatt gaaggaagag cttggaaaac taaaagggaa atcagaaagg tcatctgagg
      481 accccactga tgactccagg cttcccaggg ccgaagcgga gcaggaaaag gaccagctca
      541 ggacccaggt ggtgaggcta caagcagaga aggcagacct gttgggcatc gtgtctgaac
      601 tgcagctcaa gctgaactcc agcggctcct cagaagattc ctttgttgaa attaggatgg
      661 ctgaaggaga agcagaaggg tcagtaaaag aaatcaagca tagtcctggg cccacgagaa
      721 cagtctccac tggcacggca ttgtcta
//
