LOCUS       sequence-289517-        8097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8097)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAGO3_O
FEATURES             Location/Qualifiers
     source          1..8097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5382..5423
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5433..5450
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5473..5490)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5523..5657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5547..5566)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5601..5620
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6279..6867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6359..6378)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7038..7829,7830..7898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7661..7680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7899..8003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8071..8089
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgtctggaag
     2761 aggaaatttg ttgagcttat ttaataaaaa tgctggcaat atgggaaaat caatttcaag
     2821 taaagatcat gaaatcgaca gtggcttgga ttttaacaac tctgaatcaa gtggagaacg
     2881 tttactgtca agccataata ttgagactga tcttataacc actcttcaac atgtaaacat
     2941 ctcggtcggc cgaggacgtg ctaggcttat agacacatta aaaacagatg accatacatc
     3001 aaatcaattt ataacatctg aatcaaagga aaacattacc aaaaagacaa agggccccga
     3061 accggaagca attgcttcag aaaatggatt attttttccc gatttaattt acggatctaa
     3121 aggttcatct gttaatatat attgtaatta cttaaagcta acgacggatg aatccaaggg
     3181 agtttttaat tacgaagtgc gtttttttcc tcccattgac tctgttcatt taagaataaa
     3241 atatttaaat gatcataagg ataaactcgg tggaaccaag acatttgacg gaaatacttt
     3301 atatttacct attttgttac caaataagat gaccgtcttt attttaaagg cggaagatgt
     3361 tgagttgcag attcgcattt tatacaaaaa aaaagaggaa atgaggaact gcacgcaatt
     3421 atataatatt ttattcgata gagttatgaa agtattgaat tacgtaaaat ttgatcgcaa
     3481 gcagtttgat ccttcgcgtc caaaaataat accactggca aagttagaag tttggcctgg
     3541 ctatgttaca gccgttgacg aatacaaagg tggtttaatg ctatgctgcg acgtttcgca
     3601 tcgtatactt tgccaaaaaa ctgttctaga aatgctggtt gatctttatc agcaaaatgt
     3661 agagcattac caagaatcag caagaaaaat gttggttgga aatattgtct taactaggta
     3721 taacaatcgt acatataaaa taaatgacat ttgttttgat caaaatccaa catgtcagtt
     3781 tgaaattaaa actggctgca caagttacgt ggagtattac aaacaatatc acaatataaa
     3841 cataaaggac gttaatcaac cattgattta cagtataaag aaatcaagag gaattccagc
     3901 agaaagggaa aatttacagt tttgtttaat tccagaattg tgttatctta ctgggctgcg
     3961 agacgaagta cggtcagata acaagcttat gcgcgagatt gctacattta caagagtttc
     4021 gccaaatcaa cgacagatgg cgttaaataa attttacgaa aatgtttcaa atacacccgc
     4081 cgcacaagaa attctaaata gctggggtct ttcattaacg aataattcca acaagataag
     4141 tgggcgtcaa atggacatag agcaaatata tttttcaaaa attagtgtat cggccggaag
     4201 gtccgcagag ttctccaaac atgctgtgac caatgaaatg ttgaaagtag tccacctgag
     4261 taaatggatc attatacatt taagaaatta tagacaagct gcaactagct tgctcgataa
     4321 catgaaacag gcttgtgagt cacttggtat gaacatttct aacccaacaa tgatttcgtt
     4381 agatcatgac cgaatcgatg cctacattca agcactgcgg cgaaatataa ctatgaatac
     4441 gcaaatggtc gtatgtattt gtcataacag aagagatgat cgttatgctg caataaaaaa
     4501 aatatgttgc tcggaaatac ctataccatc acaggttata aatgctaaaa ctttacaaaa
     4561 tgatcttaaa attcgatccg ttgttcaaaa aattgttcta caaatgaact gtaaactcgg
     4621 aggctcacta tggacggtaa aaattccatt taaaaatgta atgatttgcg gaatagactc
     4681 atatcatgac ccatctaatc gggggaattc tgttgctgca tttgtggcct ccataaattc
     4741 gtcgtattca cagtggtaca gtaaagctgt tgtacaaaca aaacgtgaag aaattgttaa
     4801 tgggctctct gcatcttttg aaattgcatt aaaaatgtat cgtaaaagga atggcaaact
     4861 ccccacaaat attataatat acagagatgg aattggagat ggacagcttt atacatgtct
     4921 taattatgaa attccacaat ttgaaatggt gtgtggaaat cgcataaaaa tttcatatat
     4981 tgtagtccaa aaacgaatta acactcgcat cttctcgggt agcggcattc atctagaaaa
     5041 cccattgcca ggaactgtcg tagatcaaca tataacaaaa tcaaatatgt atgatttttt
     5101 tttggtttcg caattggtac gacagggtac tgtaacccca actcattatg ttgttcttcg
     5161 agatgactgc aattatggac ccgacataat tcaaaaacta agttacaaac tttgtttttt
     5221 atattacaat tgggctggta cagtacgaat tccagcttgt tgtatgtacg ctcacaaatt
     5281 ggcttatttg attggacaaa gtattcaacg agatgtggct gaagcacttt ctgaaaaact
     5341 attttatctt taactcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     5401 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa
     5461 cccgctgatc agcctcgact gtgccttcta aggcctgagc tcgctgatca gcctcgatcg
     5521 aggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     5581 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     5641 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     5701 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     5761 atcagtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     5821 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     5881 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5941 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
     6001 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
     6061 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
     6121 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     6181 gtagatgacg accatcaggg acagcttcaa ggatcgctcg cggctcttac cagccagcaa
     6241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     6481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7321 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     7381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7621 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     7681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8041 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
