LOCUS       sequence-289519-        7921 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPiwi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7921)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPiwi_O
FEATURES             Location/Qualifiers
     source          1..7921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5206..5247
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5257..5274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5297..5314)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5347..5481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5371..5390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5425..5444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6103..6691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6183..6202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6862..7653,7654..7722))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7485..7504
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7723..7827)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7895..7913
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttcatgc agcgggtgaa agtattcaga ggatcttcat caggtgaccc
     2761 gagagcggat cctcgtatag aggcttcaag agagagagga gctctcgagg aagctcccag
     2821 gcgtgaaggt ggcccgccag agcgaaagcc gtggggtgac caatatgatt acctggatac
     2881 ccgtccggtt gagctggtat ccaagaaggg aaccgatggc gtcccggtca tgctgcagac
     2941 gaactttttt cgattaaaaa ccaagccgga atggcggatc gttcattatc acgtggagtt
     3001 tgtgccgacc atcgagaatc ctcgtgtccg tatgggagtt ttgtccaatc atgctaacct
     3061 tctgggatca ggctatctat tcgacggact gcaactgttc accaccagga aattcgagca
     3121 ggaaatcacg gtgctcagcg gaaagtcgaa gctggacatt gaatacaaga tatccataaa
     3181 gttcgttgga ttcatatcgt gtgctgagcc ccgctttttg caagtcttaa atctaatatt
     3241 gcgccgctcg atgaagggcc taaatttgga attagttggc cgtaatctct ttgatccccg
     3301 agctaagatc gaaataaggg agttcaaaat ggagctatgg ccgggctatg agacatcgat
     3361 tcgtcagcac gaaaaagata ttttattggg caccgaaata actcacaaag ttatgcgcac
     3421 cgagacgatc tacgacataa tgcgacgttg ctcacacaat ccggctcgtc atcaggacga
     3481 agtacgggta aatgttttgg acttgattgt ccttacggat tacaataaca gaacttatcg
     3541 tatcaatgat gtcgactttg gacaaactcc gaaatcaaca ttcagttgca agggtagaga
     3601 tatcagtttc gtggaatact atctcactaa atataatata cgcattcgcg accacaatca
     3661 gccgctgctg atttccaaaa atagggacaa ggctctaaaa actaacgcta gcgaattagt
     3721 ggtactaatt cctgagctct gccgagtgac tgggctcaat gccgagatgc gctcaaactt
     3781 tcagcttatg cgtgccatga gcagttatac gcgaatgaac cccaaacaac gcactgatcg
     3841 attgcgcgct tttaaccacc gtttacaaaa cactccagaa agtgtgaagg tcttgagaga
     3901 ctggaacatg gaactggaca agaacgtcac agaagtacaa ggccggataa ttggacagca
     3961 gaacatcgtg tttcataatg gaaaggttcc tgctggagaa aacgctgatt ggcaaaggca
     4021 cttcagagac caaaggatgc ttaccactcc gagcgatggc ctcgatcgtt gggctgtcat
     4081 tgcgccgcaa aggaattccc atgaactccg aactctactt gactctttgt atagagcagc
     4141 tagtggaatg ggtcttagaa ttcgaagccc ccaggaattc ataatttatg atgatcgcac
     4201 tggaacttat gtgagagcaa tggatgattg tgtgcgctcg gatcccaaac ttatattatg
     4261 cctcgtaccc aatgataacg ccgaaagata ctcatcaatt aaaaagagag gatacgttga
     4321 cagggcggtg ccaactcaag ttgtgaccct taaaacgacc aagaaccgta gccttatgag
     4381 cattgccacc aaaatagcaa ttcaactgaa ttgcaagttg ggatatacac cctggatgat
     4441 cgaactaccc ttgtccggac tgatgacaat tggctttgac attgcgaaga gcacacgaga
     4501 tcggaagagg gcctacggag cattgattgc ctcaatggat ctacagcaaa actccacgta
     4561 cttcagcaca gtcacggagt gcagcgcctt tgatgtgctc gctaacaccc tttggccgat
     4621 gatagcaaag gccctgcgcc aatatcaaca tgagcatagg aagctgccat ctcgaatcgt
     4681 attttatcga gacggtgtga gctccggctc tctaaagcag ctttttgaat ttgaagtcaa
     4741 ggacatcatt gagaagttga aaactgaata cgcccgcgtc cagctaagcc caccgcaatt
     4801 agcttatatt gtggtaacca gatccatgaa cacgcgcttc tttctcaacg gacaaaatcc
     4861 tccgcctggt actatagttg atgacgttat aactctgccc gagagatacg acttttatct
     4921 ggtctcgcaa caagttcgtc agggtacagt gtcgccgacc agctacaatg ttctttatag
     4981 cagcatgggt ctctcaccgg agaaaatgca aaaacttacg tacaagatgt gccacttgta
     5041 ctacaattgg tcgggcacca cacgagtgcc agcagtttgc cagtacgcta agaagttagc
     5101 tacactcgtg ggtgcgaact tgcactctat tccgcaaaac gcgctcgaaa agaagtttta
     5161 ttatctataa gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
     5221 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
     5281 taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg atcagcctcg
     5341 atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     5401 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     5461 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     5521 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     5581 tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     5641 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     5701 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     5761 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     5821 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     5881 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     5941 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     6001 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcca
     6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6541 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6721 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6781 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6841 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6901 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6961 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     7021 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     7081 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7141 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     7201 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7261 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7321 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7381 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7441 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     7501 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7561 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7621 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7681 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7741 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7801 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7861 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     7921 t
//
