LOCUS       sequence-289611-        8835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-12xmut_1-1115.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8835)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-12xmut_1-1115_P
FEATURES             Location/Qualifiers
     source          1..8835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6120..6161
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6171..6188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6211..6228)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6261..6395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6285..6304)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6339..6358
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7017..7605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7097..7116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7776..8567,8568..8636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8399..8418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8637..8741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8809..8827
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcgtgaagc
     2761 ccttttttcc caagatgccg cggcctgtca gcatgttaac caggatacta attgggaagt
     2821 tcccagttcg ccagaaccag ccaataagga tgcacccggt ccaccaatgt ggaagccaag
     2881 cattaacaat ggtactgatc tttgggagtc caatttgaga aacggaggtc agccggccgc
     2941 acagcaagtt ccaaagccgt cggcggctca tacaccatcc tctaacttag gtggaacagc
     3001 agctgaggac gacgatggcg ccgatagtag tagtgtgtgg actggaggag ctgttagcaa
     3061 cgcgggatcc ggagctgcag tgggagtaaa ccaagccgga gttaatgtcg gtccaggcgg
     3121 tgttgtttcg tctggcggac ctcaggcggc acaaggtgtc gttggcgtcg gacttggatc
     3181 aactggaggt aacgggtcaa gcaatataac tggatcgtct ggagtcgcaa caggtagtag
     3241 cggaaactcc agcaacgctg gtaacgctgc ggcagaccct cgtgaaatac gccctttggg
     3301 agttggtggc tccatggata ttcgaaatgt tgaacatcgc ggcggtaacg gttctggagc
     3361 aacttcgagc gatccacgag acattcgcat gatcgatccg cgtgacccta ttcgaggaga
     3421 tccccgtgga atatctggtc gtcttaatgg gacctctgaa atggccgctc atcatccaca
     3481 aatgtcccat aaccagttgc aaggtatcaa caaaatggtt ggtcaaagtg tagcaactgc
     3541 cagcaccagt gtcggaacat ctggctcggg catcggtcct ggaggtcccg gtcctagtac
     3601 agtatcaggc aatatcccaa cacaggcggc gcctgctcaa ccggtaagcg ttggtgtaag
     3661 tggtcccaaa gacatgtcaa aacagataag tgcagcggag gaaccatcac caccgcctca
     3721 gcgtcgcagt attcctaact acgatgatgg tacatcgttg gcggctcagc aaactcgtgt
     3781 tcccgctgca agcggtcact ggaaagacat gactgattcg ataggtcgta gtagtcatct
     3841 catgcgtggc caaagccaaa cgggaggtat aggaatagcc ggcgttggaa atagcaatgt
     3901 tccagtggga gccaatccaa gtaatcctat aagcagtgta gttggacctc aagcccggat
     3961 tccatctgtg ggcggcgtac aacacaaacc agacggcggc gctatgtggg tgcattccgg
     4021 caatgtaggt ggcagaaata atgttgctgc tgttactact gcagcggatg acactcatag
     4081 cgttaatgtc ggcgctccca gcagtggcag tgtatccagc aacaattggg ttgatgacaa
     4141 gtccaactca accttggcac aaaactcttg gagcgacccg gcccctgttg gagttagtgc
     4201 cgcgaataag caaagcaaac cgccaagcaa tagtgcttca tcagctgcga gcactgctgc
     4261 gggcgtggtg gatggggttg atctaggatc tgagtggaac acgcacgggg ggattattgg
     4321 aaaatctcag caacaacaaa aactagcggg acttaacgtg ggaatggtga acgtaattaa
     4381 cgcggagatc attaagcaaa gcaagcaata caggatcctt gtcgagaacg gctttaaaaa
     4441 ggaagatgta gagcgggcat tagtgattgc taatatgaac atcgaagagg cagccgatat
     4501 gctccgtgcc aactcatccc tatcaatgga tggttggcgt cgacatgatg agtcccttgg
     4561 atcttatgcc gaccacaata gttcaacaag cagcggtgga tttgctggtc gttacccggt
     4621 caacagtgga caaccttcaa tgtcctttcc tcataataac cttatgaata acatgggagg
     4681 taccgctgtt actggaggta acaacaatac aaacatgaca gctttacagg tgcaaaagta
     4741 tttaaatcaa gggcaacatg gtgtcgctgt tggaccgcaa gccgttggta attcttcagc
     4801 agtatctgtc ggatttggtc agaacacgtc taacgcagca gtggcaggag cagcctctgc
     4861 aaatatagca gcaaatacaa acaaccaacc gtctggtcag caaattcgca tgctaggcca
     4921 gcaaattcag ttggccattc atagtggttt catatctagt cagatattga ctcaaccgct
     4981 aactcaaaca acccttaacc ttttaaacca acttcttagc aatattaagc atctccaggc
     5041 tgcacagcaa tcccttaccc gcgggggaaa tgtcaatcca atggcagtga atgtggctat
     5101 atctaaatac aagcagcaaa tccagaattt acagaaccag ataagtgcac aacaggctgt
     5161 gtatgtaaaa cagcaaaata tgcaaccaac ttcacaacaa caacagcccc aacaacagca
     5221 acttccttct gttcatctaa gtaactcagg caacgactat ttaagaggtc acgatgcaat
     5281 aaataatttg caaagcaact tttctgagct caatattaat aagccaagtg gatatcaagg
     5341 agcgtccaat caacaatccc gattaaatca gtggaagctt ccagtattag ataaggagat
     5401 caactctgac agtacggaat tttctcgtgc cccaggtgca acgaaacaaa atttgacggc
     5461 caacacaagc aacataaact ctttgggtct tcaaaacgat agtacatggt caactggacg
     5521 cagtattggt gacggttggc ctgatccctc atctgataac gagaataaag actggtctgt
     5581 tgctcagcca acttcagcag caactgctta cactgatctg gtccaagagt ttgagccagg
     5641 caagccatgg aagggttcac agatcaaaag catagaagat gatcccagca ttacaccagg
     5701 aagcgttgct agatctccat tgtctattaa ttcgacgcca aaagatgctg acatatttgc
     5761 caataccggt aaaaattcac cgactgattt accgccacta agtttatcgt cgtctacatg
     5821 gagttttaat ccaaaccaaa attatccgag tcacagttgg tctgacaata gtcaacaatg
     5881 taccgccact tcggagcttt ggacaagccc gctaaataaa tcatcgtctc gaggtccccc
     5941 gccaggattg actgccaatt caaataaatc tgcaaatagt aatgcgtcaa cgccaacaac
     6001 tattaccgga ggtgcgaatg gatggttaca gcctcgaagt ggcggtgttc aaaccacaaa
     6061 cactaattgg acaggtggta acaccacttg gggctaatct agagggcccg cggttcgaag
     6121 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     6181 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc
     6241 gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa
     6301 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6361 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6421 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     6481 gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc
     6541 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     6601 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     6661 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     6721 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     6781 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     6841 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     6901 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     6961 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7201 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7441 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     8101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     8401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8761 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8821 acgaggccct ttcgt
//
