LOCUS       sequence-289612-       12978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1-3xL-Amut-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12978)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 12978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1-3xL-Amut-dsRNAres_P
FEATURES             Location/Qualifiers
     source          1..12978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10263..10304
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10314..10331
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10354..10371)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10404..10538
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10428..10447)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10482..10501
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11160..11748)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11240..11259)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11919..12710,12711..12779))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12542..12561
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12780..12884)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12952..12970
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta agatgaacgt agagagccaa ctgaagacac cgccaacgca
     2761 cattcgaaat ctggtcaagc acgtgaacaa gcgaaatttt aatgagagca gcgagcaaat
     2821 aaatcagttc gttaaagagc acggcctgga agcggatcgc agcagcctgc gccacctgtt
     2881 ctccgtgatc aacttcagcg atctcgtgcc atcagttacg gtccagctgc aggcgaagct
     2941 cctgggcatc cagttggagc gtcagcttca cagttcatcg tttgtgtcca acatctgcta
     3001 cgcttttgac cagttcttcg ccagcaacca gaagtccctg aagcctgttg cggtagcgga
     3061 tctcgttggc caggtcgccc gactgaccgg catcaacaag gtttgtgagt gcgtctttgc
     3121 cttggccttg acccactcct cgtacacaga actcaaacat tctgccagga acaacctaaa
     3181 aggtagcctc agcgaactta tagactctta cttgggtaat aaaggcaccg gcccagccga
     3241 cagcggtctc cgggagatct ccttcgatct actgcagtat ttgctgtgct gccttagcga
     3301 gtacgtacaa ccgcaggtgg aagcacagtt tttgatcaag cttcgtgagg agtttccgcg
     3361 ccaggcggta ccgctggttc tagcgccgtt cctttacggc tcgacgacgg cgacgattgc
     3421 gggagcaggt gccagcgaaa cggatgcgga ggccgaagcg acgactaata gcaacagctc
     3481 cagtttcgaa gcagatgctt tgaacgaggt gggaatcgag gatatttatg accatttaag
     3541 cgagataata ttcaccaacc agggcaaaaa taatattatg gacacatctt ggattaatct
     3601 aatccttgaa atcggttatg aatttacatc gagcgttgaa gagtgtaaga atcatttgtg
     3661 ttcccgcgag cgagaacgcg ctgagctcca gtccaaagat gtcgccaaga tcgtgggtct
     3721 tatgtgccga cggcactcct ccctgctcga ttgtaatgtg aatctaccga cgccggcgaa
     3781 cttctggcct ggtcagggac agggcggtgg cagcaacagc agtggctctt cgcagacaca
     3841 aatcactccg cagcaacaga acccgggcag cagtaacaac aacgatggca gcgatggcaa
     3901 ttcgtccagt gataagaagg acaagaagga gacgacggag gccacgcaaa cttggaagcc
     3961 agatgtcttt gtgcaggcgc tcaaggaggt ggtgcctcag ctcaactgga aggacgtgtg
     4021 catggaactt gatcatcccg agttcgtgct gaaggatcgc atcggtttgg agcttttgct
     4081 caccatcctt cggctggcca ctggctctaa catatttcca catccagaat gcatttaccg
     4141 tcactgggca aacacggaag gtcagctgtc gcttatcgcg acaatgctga agaacccgga
     4201 tctcttttcc ttcgccgact ttgtatttag ccagccagcg ttggatgtgc ttaagacagc
     4261 tccggatgcg gacaataagg agatctcgtc ctggaagtcg cttcacctgg tggaggtgct
     4321 gctctccatt gcggacaagg gatactatac gcaggtccat gagctattca agttcccagc
     4381 gcagaactgt cccgatgtgc tatttttggc cttattgaac acaagtccgc ctatgacgcc
     4441 actgcgtcag gatctgttta accaactgat accaacgttc cttggcaacc atcccaactc
     4501 gaacgtgatc ttggccagcg cctggagctc gaacagcttc caacttcgtt ccaatatcat
     4561 gaatgctatg tccgagtggt atctccgagg caatgagttc gatcaggtga aactgtcgcg
     4621 catcctggat cttgcccagg atttaaaagc gctatcagcg ctgctcaacg cccgttcatt
     4681 tttattcatt atcgacctgg cttgccttgc ctctcggcgc gagtacctga agctggaaaa
     4741 gtggcttacc gataaaattc gcgaacatgg agagcctttt atgcaggcta taatcaaggt
     4801 tctgcaccgc cgctgcccgc aggttatcaa cgccaaagtg ccagaggatc agctgcctcc
     4861 caagcaggcg cagcttttgc ccgagacagt tacaaccatg atcaactgtc tgcaaacgtg
     4921 catcaataac tgcatgcagc cggagatggt cgaagtcatc atgcaaatga ccgcgaacgt
     4981 ggcgattatg gctaacatgg cgcgtgccca acagcagcag cagccaggat tggttccgcc
     5041 gcctccacca acaattctgc gcggccatcg tggtatggat ctgcctggag gcatagtccc
     5101 tccgccaccg cagcaaccgt tctccggaaa cctcaacgcg cagatgtttg gaccaggaat
     5161 ggatccgcta acgaatatgt ccaacaactt agccggcctc aacctcagcg gcccgaacgg
     5221 agcatttaat tttggaaaca tgctaacttc tccatcgcgt ctgatgactc cgggagccaa
     5281 tccctatccg ctgaacctca tgcagatgcc gcaggctccg ccaccaccca acgtaggaaa
     5341 cctaggccgc atgctgccag ggggtccaca acagcagact ccaacgccga ctccaacggc
     5401 ccccaatcca aacaaccctg tgatggccga tctccagata cctgtgtcaa aggaagttga
     5461 agacgaggtc aactcatact ttcagcgcat ctacaaccac cagccgaatc caacgctgtc
     5521 catcgatgaa gtgctggaca ttttgcaaag atttaaagag tcaagtaacc ggcgcgagca
     5581 ggaagtcttt ttgtgcatgc tgcgcaatct gttcgaggaa tatcgctttt tctgtcaata
     5641 cccagagaaa gagctacaga ttactgcgca gctatttggt ggcattattg atcgtaacct
     5701 ggtgcccact tttgttgcac tgggcctttc tctgcgctgt gtcttggatg ccctccgcaa
     5761 gcccgatgga tctaagcttt actatttcgg tgtgacggca ctggacagat tcagaactcg
     5821 attgcacacc tacaacaaat actgcgagca tatccgctcc attcctcact tctctgactt
     5881 cccgccgcac cttattcagt atgtggaata cggaatgcac ggccaggagc cgccgccgca
     5941 gaagttgatt ggactcagca atacgattcc ttcggcgata tctagcggac caggaacgga
     6001 accaatttac aggaatagct ccatgttagg taatatgccc gctgccaccc cgggatcggg
     6061 accgaaatca aacgctgctg tatcgcatgc cacgagaatg aagtccatcg ccaatgccac
     6121 caatatcgac acacttttgg tggccaacca ggaagagaaa gtgactgtgc caccagagcc
     6181 tgtgcaggac aaaactgcct ttattttcaa caacttgagc cagctgaaca taccgcagaa
     6241 gtgcgatgag ataaaggaga ttatgaccaa ggagtattgg ccgtggctag cgcaatatct
     6301 ggtcctcaaa cgcgcatcaa tggagttcaa cttccacaca ctatattaca actttttgga
     6361 tgcccttaaa aacggcgaga tcaaccgatt cgtaaccaaa gagaccctac gcaacatcaa
     6421 ggtactcttg cgctccgata agggagttat taatttctcc gatcgaagtc tgttgaagaa
     6481 cctcggacac tggttgggca tgatgacctt aggtcgcaat cgccccatcc tgcagttgga
     6541 tctggatgct aaatccgctg cggctgaggc ttaccacaag ggccagcagg aactgctttt
     6601 tgtggttcct ttcgtagcca agatccttga gtcatccgcc aagtctcgca tcttccgatc
     6661 gcccaatcca tggacaatgg gtatcatgta cgtgcttgct gagcttcacc aggagccaga
     6721 ccttaagctg aacctgaagt ttgaaatcga agtactctgc aaaacactta atctggagtt
     6781 ggccaagttg cgacaagtga tctacctgaa ggatcccaat cgaactcacc ggattgaaga
     6841 gcagatgtcg caaccaaaac cgaaacaact tgagccagtg gcttctgcgc cagctctacc
     6901 gcgtgaacag caatccccgg cacaacctcc gccaccacca caacagcagc aaccaccgca
     6961 gcagcaggta cctccaccgc catcctctgc ggacgtggac gcccaaaatg cggccgctat
     7021 gatgatggca gcgggcggag ctaatagtac tcctggatcg gtatcttcgc ccaatctacc
     7081 caccgattcc agccaggtgg cactaccgcc accagagccg cgctattcat acgtggacgt
     7141 aaacgtgagc aacttccagc ttattggtca gcagttggta ctgccaccca atactccgtt
     7201 cctgcacgcc aatcccggaa tcaaacacat ggtcgtcaac gccgtggagc gcacgataac
     7261 cgactggttg cagcctattg tggaccgaag catccgcatt gcctgtgcca ccaccgagca
     7321 gatcatccgc aaggacttcg ccctggatgc tgacgagaac cgaatgcgca ctgccgctca
     7381 tcagatggta cgaaaccttg ccgccggcat ggccatgatt accggtaagg atgagatagc
     7441 gcgtgctatt agccagaatc tgcacaaggc cttgctgtcg ggtctaaatg ggatgcccag
     7501 catggctgag atccaagctg ctgctatgca gttagccagc gaaaatgttg agctagtttg
     7561 cgctttcatt cagaaaacat ctgctgaaaa ggcagccgca gagatcgata ggcgtctaag
     7621 caccgatttc gagaccagaa aaattgctcg tgaagagggc aaccgctttg tggacgccca
     7681 gatcctcacc tatcaacagg agcgtcttcc agaagcagtg cgcatcaagg tgggcgcggc
     7741 tccagctaca ctttatgctg tgtactcgga gttcgctaga agcatacctg gcttccagca
     7801 gatgagcgat cgcgatattg ccctgtttgt gcccaagccg acggatttgt ctcagccaaa
     7861 tgtgtttgct aatgatgata gcatggtata cggggaactg gctagcaaaa tggaggcctt
     7921 catgaacacg gcaatcggag tgccgacact ccagatccag gctagcaaga tgcacatgct
     7981 actcaacgcc ctgatagcga cgcgtcgact gcgtgaccaa gagtctgcct ttaatctgct
     8041 gacccgcgcg gtcgagggtt tgaccgaagg attggtcaat atgcatgaaa acatggagca
     8101 aatgaagatg taccaaaaca tccatctccg tatccttggc ttgctgaaca acagtttcgg
     8161 tgctccaaac acagagcgag cggtaacaaa gtgtttcttc gatatccgag aggaggtgcg
     8221 atataacgtg gaggcagctc gcgctttgat tacgtcgcac tttgtcaatc tgaatcagtt
     8281 tgacggaatg ctacgcgact gcatggacaa cggaaacaat tatgtggcta tatcgttcgg
     8341 tatcgcactg ctggagcgcc tcatcatgga cgatcgagtg attaatattg tttcggacaa
     8401 cgaattcatg gcaactgttg agctgctagg taggctgacc caacaccgtc accgctaccc
     8461 cgagtgtatc gtcaacgcca tcgacaccct gtggtccggc aatttcaata gctcctccga
     8521 ttattccccc tttaatggaa acgaccgcta tctgagcggc gctagccact atattcatag
     8581 cggaatgcac catgtccgct cgtgtgacac cgacgacccc cccggactcc aggaaaaaac
     8641 cgaattcttg ttgaaagact gggtggcctt gtatacccag caaaatcaac aatcgacccg
     8701 tgacgcccgt aattttggcg ccttcgtgca aaaaatgaat acctacggca ttttgaagac
     8761 cgatgatctg attacgcgct tctttcgtca agctacccat atctgtaccg acgtcgtcta
     8821 tcgtatgttt gctgaaccca gccttcccat taatcaagct aagaataaaa tttttcaatg
     8881 gattgacgcc tttgtgcact tgatcgccat gctggtccgc cattcgggag aggccggcaa
     8941 ccccacgacg aaaattaatt tgctcaataa agtcttggga atcgtcttgg gcaccttgat
     9001 caaagatcac gaaatgcgcg gagtgtcctt tcaacaagtg ggatatcacc gtttttttat
     9061 gatgttgttt atggaattgt gtaccgctga cgtcatcctg gaaagcttga tgcatagcat
     9121 cgtcagcgct tttgcttaca catatcactt gttgaatccc agcgtggctc cgggattctg
     9181 ctttgcttgg ttggagctca tttcgcatcg cgtcttcctg ggccgcatcc tggtccaaat
     9241 tcccgggcaa aagggctggc cactttacgc tcaattgctg caggaccttt ttaaatatct
     9301 tgcgcccttc ttacgcaaca ctgagctcgg caaaccagtc cagcttttgt acaaggggac
     9361 gctacgtgtt ctgctcgttc tgcttcacga cttcccggag ttcttgtgcg actatcattt
     9421 tgggttctgt gacaccatcc cgcccaattg cgtccagatg cgtaacatca tcctgtcggc
     9481 gtttccgcgc aacatgcgct tgcccgatcc cttcacgccc aacctaaaag tggacatgct
     9541 gtcggacagt agtaatgcgc ctaaggtgct cagtagctac attatgaaca tccagcccgc
     9601 gaactttaag aaggatttgg actcgtacct caaggcccgg gcgccagtta cttttctatc
     9661 ggaattacgt ggtcacttgc aggtgacaag cgagccagga actcgttaca acatggcgct
     9721 gatgaatgct ctggtcatgt atgtgggaac tcaggcgatt gctttgatca gaaacaaaaa
     9781 ctttgtgccc aacacctcga acatagcaca cagcgcccac atggatatct tccagaatct
     9841 tgctgttgac ttggatacgg agggtcgcta tctgttcttg aatgcgattg cgaaccagtt
     9901 gcgctatccc aacagccaca cgcactactt cagctgtgcg gtgctgcatc tgtttgccga
     9961 ggccaactcg gaggcaattc aggagcagat cactcgcgta cttctggaac gattgatcgt
    10021 gaaccgcccg catccttggg gactgttgat cacttttatt gagctgatca aaaatcccat
    10081 ctacaagttc tgggaccatg actttgtgca ctgtgcgcca gagatcacca agctctttga
    10141 atcggtggcc cgttcctgtc tggccaagtc gaacgtcacc cagcagttga acatgcccgt
    10201 cgtcgatggc gagggccagg aggtagccac catcaactga tctagagggc ccgcggttcg
    10261 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
    10321 accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag
    10381 ctcgctgatc agcctcgatc gaggatccag acatgataag atacattgat gagtttggac
    10441 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
    10501 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
    10561 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
    10621 aatgtggtat ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc
    10681 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
    10741 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
    10801 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
    10861 ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
    10921 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
    10981 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
    11041 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
    11101 gcggctctta ccagccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    11161 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    11221 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    11281 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    11341 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    11401 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    11461 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    11521 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    11581 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
    11641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    11701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11761 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
    11821 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11881 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
    11941 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    12001 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    12061 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    12121 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    12181 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
    12241 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    12301 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    12361 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    12421 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    12481 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
    12541 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    12601 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    12661 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12721 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12781 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12841 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12901 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
    12961 atcacgaggc cctttcgt
//
