LOCUS       sequence-289695-        9730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsXRN1_1-1649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Kuzuoglu-Ozturk D, Huntzinger E, Schmidt S, Izaurralde E
  TITLE     The Caenorhabditis elegans GW182 protein AIN-1 interacts with PAB-1 
            and subunits of the PAN2-PAN3 and CCR4-NOT deadenylase complexes.
  JOURNAL   Nucleic Acids Res. 2012 Jul;40(12):5651-65. doi: 10.1093/nar/gks218.
            Epub 2012 Mar 7.
  PUBMED    22402495
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks218"; journalName: "Nucleic Acids Res"; date: 
            "2012-07"; volume: "40"; issue: "12"; pages: "5651-65"
COMMENT     Sequence Label: pT7-EGFP-C1-HsXRN1_1-1649_P
FEATURES             Location/Qualifiers
     source          1..9730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6518..6639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6555..6574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6609..6628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6646..7101)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6783..6804)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6995..7014
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7128..7232
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7234..7591
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7234..7425
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7257..7277)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7442..7577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7626..8420
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7626..8420
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7680..7699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8290..8309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8608..8627)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8652..8699
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9028..9616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9517..9536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggcat
     1441 gggagtcccc aagttttaca gatggatctc agagcggtat ccctgtctca gcgaagtggt
     1501 gaaagagcat cagattcctg aatttgacaa cttgtacctg gatatgaatg gaattataca
     1561 tcagtgctcc catcctaatg atgatgatgt tcactttaga atttcagatg ataaaatctt
     1621 tactgatatt tttcactacc tggaggtgtt gtttcgcatt attaaaccca ggaaagtgtt
     1681 ctttatggct gtagatggtg tggctcctcg agcaaaaatg aaccagcagc gtgggaggcg
     1741 ttttaggtca gcaaaggagg cagaagacaa aattaaaaag gcaatagaga agggagaaac
     1801 tcttcctaca gaggccagat ttgattccaa ctgtatcaca ccaggaactg aatttatggc
     1861 caggttacat gaacatctga agtattttgt aaatatgaaa atttccacag acaagtcatg
     1921 gcaaggagtt accatctact tctcaggcca tgagactcct ggagaaggag agcataaaat
     1981 catggaattt atcagatccg agaaagcaaa gccagatcat gatccaaaca ccagacactg
     2041 tctttatggt ttagatgctg acttgattat gcttggatta acaagtcatg aggcacattt
     2101 ttctctctta agagaagaag ttcgatttgg tggcaaaaaa acacaacggg tatgtgctcc
     2161 agaagaaact acatttcacc ttctacactt gtctttaatg agagagtata ttgactatga
     2221 gttttcagta ttaaaagaaa agatcacatt taaatatgat attgaaagga taatagatga
     2281 ttggattttg atggggtttc ttgttggtaa tgattttatc cctcatctac ctcatttaca
     2341 tattaatcat gatgcactgc ctcttcttta tggaacatat gttaccatcc tgccagaact
     2401 tgggggttat attaatgaaa gtgggcacct caacttacct cgatttgaga aataccttgt
     2461 gaaactatca gattttgatc gggagcactt cagtgaagtt tttgtggacc taaaatggtt
     2521 tgaaagcaaa gttggtaaca agtacctcaa tgaagcagca ggtgtcgcag cagaagaagc
     2581 caggaactac aaggaaaaga aaaagttaaa gggccaggaa aattctctgt gttggactgc
     2641 tttagacaaa aatgaaggcg aaatgataac ttctaaggat aatttagaag atgagactga
     2701 agatgatgac ctatttgaaa ctgagtttag acaatataaa agaacatatt acatgacgaa
     2761 gatgggggtt gacgtagtat ctgatgactt tctggctgat caagctgcat gttatgttca
     2821 ggcaatacag tggattttgc actattacta tcatggagtt cagtcctgga gctggtatta
     2881 tccttatcat tatgcgcctt tcctgtctga tatacacaac atcagtacac tcaaaatcca
     2941 ttttgaacta ggaaaacctt ttaagccatt tgaacagctt cttgctgtac ttccagcagc
     3001 cagcaaaaat ttacttcctg catgctacca gcatttgatg accaatgaag actcaccaat
     3061 tatagaatat tacccacctg attttaaaac tgacctaaat gggaaacaac aggaatggga
     3121 agctgtggtg ttaatccctt ttattgatga gaagcgatta ttggaagcca tggagacatg
     3181 taaccactcc ctcaaaaagg aagagaggaa aagaaaccaa catagtgagt gcctaatgtg
     3241 ctggtatgat agagacacag agtttatcta tccttctcca tggccagaaa agttccctgc
     3301 catagaacga tgttgtacaa ggtataaaat aatatcctta gatgcttggc gtgtagacat
     3361 aaacaaaaac aaaataacca gaattgacca gaaagcatta tatttctgtg gatttcctac
     3421 tctgaaacac atcagacaca aatttttttt gaagaaaagt ggtgttcaag tattccagca
     3481 aagcagtcgt ggagaaaaca tgatgttgga aatcttagtg gatgcagaat cagatgaact
     3541 taccgtagaa aatgtagctt catcagtgct tggaaaatct gtctttgtta attggcctca
     3601 ccttgaggaa gctagagtcg tggctgtatc agatggagaa actaagtttt acttggaaga
     3661 acctccagga acacagaagc tttattcagg aagaactgcc ccaccatcta aagtggttca
     3721 tcttggagat aaagaacaat ctaactgggc aaaagaagta caaggaattt cagaacacta
     3781 cctgagaaga aaaggaataa taataaatga aacatctgca gttgtgtatg ctcagttact
     3841 cacaggtcgt aaatatcaaa taaatcaaaa tggtgaagtt cgtctagaga aacagtggtc
     3901 aaaacaagtt gttccttttg tttatcaaac tattgtcaag gacatccgag ctttcgactc
     3961 ccgtttctcc aatatcaaaa cattggatga tttgtttcct ctgagaagta tggtctttat
     4021 gctgggaact ccctattatg gctgcactgg agaagttcag gattcaggtg atgtgattac
     4081 agaaggtagg attcgtgtga ttttcagcat tccatgtgaa cccaatcttg atgctttaat
     4141 acagaaccag cataaatatt ctataaagta caacccagga tatgtgttgg ccagtcgcct
     4201 tggagtgagt ggataccttg tttcaaggtt tacaggaagt atttttattg gaagaggatc
     4261 taggagaaac cctcatggag accataaagc aaatgtgggt ttaaatctca aattcaacaa
     4321 gaaaaatgag gaggtacctg gatatactaa gaaagttgga agtgaatgga tgtattcatc
     4381 tgcagcagaa caacttctgg cagagtactt agagagagct ccagaactat ttagttatat
     4441 agccaaaaat agccaagagg atgtgttcta tgaagatgac atttggcctg gagaaaatga
     4501 gaatggtgct gaaaaagttc aagaaattat tacttggcta aaaggacatc ctgtcagtac
     4561 tttatctcgt tcttcttgtg atttacaaat tctggatgca gctattgttg agaaaattga
     4621 ggaagaagtc gaaaagtgca agcaaagaaa gaataataag aaggtgcgag taacagtgaa
     4681 accccatttg ctatacagac ctttagaaca gcaacatgga gtcattcctg atcgggatgc
     4741 agaattttgt ctttttgacc gtgttgtaaa tgtgagagaa aacttctcag ttccagttgg
     4801 ccttcgaggc accatcatag gaataaaagg agctaataga gaagccgatg tactatttga
     4861 agtattattt gatgaagaat ttcctggagg gttaacaata agatgctcac ctggtagagg
     4921 ttatcgactg ccaacaagtg ccttggtgaa cctttctcat gggagtcgct ctgaaactgg
     4981 aaatcagaag ttgacagcca tcgtaaaacc acaaccagct gtacatcaac atagctcaag
     5041 ttcatcagtt tcctctgggc atttgggagc cctcaaccat tcccctcaat cactttttgt
     5101 tcctactcaa gtacctacta aagatgatga tgaattctgc aacatttggc agtccttaca
     5161 gggatctgga aagatgcaat actttcagcc aactatacaa gagaagggtg cagttctacc
     5221 tcaagaaata agccaagtaa atcaacatca taaatctggc tttaatgaca acagtgttaa
     5281 atatcagcaa agaaaacatg accctcacag aaaatttaaa gaagagtgta agagtcctaa
     5341 agctgagtgt tggtcccaaa aaatgtccaa taagcagcct aactctggaa ttgagaactt
     5401 tttagcatct ttgaatatct ccaaagaaaa tgaagtacag tcatctcatc atggggagcc
     5461 tccaagtgaa gagcatttgt caccacagtc atttgccatg ggaacacgga tgcttaaaga
     5521 aattctaaaa attgatggct ctaacactgt ggaccataag aatgaaatca aacagattgc
     5581 taatgaaatc cctgtttcct ctaacagaag agatgaatat ggattaccct ctcagcctaa
     5641 acaaaataag aaattagcat cttatatgaa caagcctcac agtgctaatg agtaccataa
     5701 tgttcagtct atggacaata tgtgttggcc tgcccccagc cagatccctc ctgtatccac
     5761 accagtaact gaactttctc gaatttgttc ccttgttgga atgccacaac ctgatttctc
     5821 ctttcttagg atgccacaga caatgaccgt ttgccaagta aaattatcta atggcttact
     5881 ggtacatggg ccacagtgcc actctgaaaa tgaagccaaa gagaaagctg cactttttgc
     5941 tttacaacag ttgggctcct taggcatgaa tttccctttg ccttcacaag tatttgcaaa
     6001 ttatccttca gctgtaccac ctggaaccat tcctccagcc tttcccccac ctactggctg
     6061 ggatcactat ggaagcaact atgcattggg ggcagctaat ataatgcctt cgtcgtctca
     6121 tctctttggc tcaatgccat ggggaccatc ggtgccagtt cctgggaagc ccttccatca
     6181 tactttatat tctgggacca tgcccatggc tgggggaata ccagggggtg tgcacaatca
     6241 gtttatacct ctgcaggtta ctaaaaaaag ggttgcaaac aaaaagaact ttgagaataa
     6301 ggaagcccag agttctcaag ccactccagt tcagactagc cagccagatt cttccaacat
     6361 tgtcaaagta agtccacggg agagctaagg atccaccgga tctagataac tgatcataat
     6421 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     6481 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     6541 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6601 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta
     6661 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     6721 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     6781 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     6841 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     6901 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     6961 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     7021 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     7081 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7141 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7201 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     7261 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7321 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7381 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7441 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7501 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7561 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     7621 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     7681 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     7741 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     7801 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     7861 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     7921 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     7981 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     8041 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     8101 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     8161 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     8221 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8281 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     8341 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     8401 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     8461 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     8521 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     8581 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     8641 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     8701 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     8761 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     8821 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     8881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9001 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9061 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     9181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9721 cgccatgcat
//
