LOCUS       sequence-289701-       10211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmXRN1-P1341A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10211)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1A-DmXRN1-P1342A-V5His6_Q
FEATURES             Location/Qualifiers
     source          1..10211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             7496..7537
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7547..7564
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7587..7604)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7637..7771
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7661..7680)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7715..7734
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8393..8981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8473..8492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9152..9943,9944..10012))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9775..9794
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10013..10117)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10185..10203
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacctactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgcccaa
     2641 catgggcgtt cccaagttct ttcgctacat cagcgagcga tatccgtgcc tcagcgaatt
     2701 ggcgcgggag cactgcatac ccgagttcga caatctgtac ttggacatga acggcattgt
     2761 ccacaactgc tcgcatccgg acgacaacaa catacacttt cacctggagg aggagcagat
     2821 attccaggaa atcttcaact acgtagacaa gctgttctac ctgatcaagc cgcagcgcct
     2881 cttcttcctt tcagttgacg gggtggctcc acgggccaag atgaaccagc agcgcagccg
     2941 gcgattccgg acggcgcgcg aggcggagca gcaggaggcc aaggcggccc agcgaggcga
     3001 gttgcgagag cacgagcgct tcgacagcaa ctgcattacg ccgggcaccg aattcatggt
     3061 gcgtctgcag gagggactgc gcgcctttct caaaacgaag atttcgacgg atccgctgtg
     3121 gcagcgttgc accgtaatcc taagtggcca ggaggcgccc ggcgagggcg agcacaagat
     3181 catggactac attcggtaca tgaagacgca gccggactat gatcccaata cacgacactg
     3241 cctttacggc ctggacgccg atctgatcat tctgggcctc tgcacccacg agctgcactt
     3301 cgtggtgctg cgcgaggagg tcaagttcgg gcgcaacgtc aagcgcacct cggtggagga
     3361 gacgcgcttc ttcctgctgc acctgggtct gctgcgcgag tacctcgagc tggagttcga
     3421 cgccctgaga acagacgagc acaaattgga cattgcgcag cttatcgacg actgggtgct
     3481 gatgggcttt ttggtcggca acgacttcat tccccacctg ccgtgcctgc acatttcatc
     3541 aaatgcactg ccgctgctct accgcaccta catcggcatt tacccgaccc tcggcggcaa
     3601 catcaacgag aacggcaagc tcaacctgcg ccgcttgcag attttcatat cggccctcac
     3661 cgaggtggag ctggatcact tcaaggagca cgccgacgac ttgaagtaca tgaacaacaa
     3721 atcagaggcg ttcgacatgg acgtcggcga gatcaccgag tctcaaaacc tcgactccga
     3781 tctcggagcc ctcatcaaca agagcatgct gctctacgat gacgacagcg aggaggactg
     3841 ctccgacgag aacgcggttc ttctgaagga gttccagaac tacaagcgaa acttctaccg
     3901 caacaagttc aagcgtgatc cgaatgatga actgatcgag gaactgtgcc accactacgt
     3961 caatgcgctg cagtgggtgc tcgactacta ctaccgtggc gtgcagtcct gggactggta
     4021 ctatcccttc cactacactc cattcatcag cgacctcaag aacatcgagc aggtggagat
     4081 cgccttccac atgggcacgc cctttctgcc attccagcag cttctggcgg tactgccggc
     4141 ggccagtgca aagcttctcc ctgtcgccta ccacgacctc atgctgctgc cgaccagccc
     4201 gctggccgag ttctatccac tggagttcga gagcgacctc aatggcaaga aacacgattg
     4261 ggaggcggtt gtgctgatac ccttcatcga tgagggtcgg ctgctggccg ctatgctgcc
     4321 atgcgaggct cagctgtcgc tggaggagcg cgaacgcaac cgacacggtc ccatgtatgt
     4381 gtacaaatac agtacggtgg cgcagggtcc catgccagcg tatccaccgc tgagggctct
     4441 tcccgtgctc tactgcacgg aggtggccaa gtggtcgcac gaaatcgcag tcaatctgcc
     4501 gtacagcgtg tgcatcgagc taccaaatgc ggcgagaacc gttttcttcc cgggatttcc
     4561 caccatgcag catctgccct tcgatttcga gctgcgcaac gaccgcgtca aggtgttcga
     4621 acaggtcagt cgcaaccaga acattgtgct aaaaccacgc aagcgccagc tggaggacac
     4681 actgacggcg gtggcctccc agtacctcgg gaaggtgatc cacgtcggct ggccgcatct
     4741 cgtcaaggcg atagtggtgc gcgtggccac gcgagatcag cgcgtcgaca gcgagggaat
     4801 cacgctaaac gattcgcggc gtttcgactc ggaatgcaag gcactgcagg aacactttat
     4861 caatcgtatg ggaatacagt ttgccaacta tgatgtgctc gtctatgtgc gcacctttgc
     4921 cggcaattcg acggagttcc gcgacaaggg ggcattgatg gtgcgcgact cgtggagcag
     4981 ctccgtcacc ggctatccag cccagggcgt ggtggccgat ctgactgtgt gggaaagaat
     5041 gcgcaagaat ttcctcaacg tggagcacta cttccccgtt ggcagcacga tcttcttgat
     5101 cacggatccg tattacggca gcgagggaac tgtgcaggac ccacggctgg cctacaccaa
     5161 cggccggatt caagtcagca tcatggtgcg accggaaccg aaagtaaatg cggcacgtca
     5221 gctgcaggaa gagcgggacc gggactatct gagcaccttc caggtctgca acttgctccg
     5281 aatcagtggc cgcaccctgg gacgcctaag tggcaccgtg tgggtggtgc tcggtccgcg
     5341 gcgtcagaag atggaaaacg tgaccaagca caacattggc ctgcagctca agtatccgcg
     5401 gcagaacgag gagcgtgccg gatactgctt ccgcacgaac aaccagtggt actactccag
     5461 cctggccgtg gatctgatgc gtaactattg ccagcgttac ccagacgtta tcgacttctt
     5521 tggcgactcc aacgatcgcg ccgagttcgt attcgaacag gacgtgttcc ccaatgcggt
     5581 tggccatcgc cgcgtcgagg aactggccaa ctgggtgcgc cagcagccgc acatgaaagt
     5641 ggagcgcatc tcttgcggct ccaagacggt ctgccgcgag accatcgaac tgctgatagc
     5701 cgccgtcgac gacctgcgct cactgcccgt caagcacgtt aaactgcagg tgaagccgca
     5761 cttgctcatc aagcccaatg tcacactgcc agatgtctac cgatcaaagc gcccggtgcg
     5821 tctcttcgat cgcgtcgtaa tcgtacgcac catttacatg gtgcccgtgg gcactaaggg
     5881 caccgtgatt ggcattcatc ccgttacgga ccccaacccg gtccgcctgg agtgcgtcca
     5941 tgctgtcgac acgttctgca aggtgctgtt cgacagcccc gttccgaact gcaacaacat
     6001 ccatggcatc gccgaagacc gagtttacaa ggtgcccgaa attgccctgg tgatcatcaa
     6061 gacggatgag gaaggaaaga agcagaacga ctgcgaactg ccggtgaggg atcctcagcc
     6121 gaaccaggca caggacgagc cagtccgcgc gaccagcagt cgctatgtga ccgccgcagg
     6181 gtccacctcg gtcccgatca cgatgaagac ccaaattagc gacgaattcg tcaagacgcg
     6241 tagcgatccg atagcccgta cggattcgta caagccatcg tcggagccaa aaccggtgcc
     6301 ggtgccggag cagattacca actggcggga acgcgtcagt acgccaacaa acaaaccgca
     6361 gcctgcgcca aataattggc gcataaatcg gtcttcatca cgtcaacagg gcggtagtat
     6421 ttttgttgct ccgcccacaa aaactcctga tgcagctgca agcactgctt ccactgcatt
     6481 caccgcagcc tcgtccgcaa cgctgactcc tctggatcag accttggcgc tcatgtcggt
     6541 tttgggcgtg ggtgaggacc agagctcgcc gccactccag gaggcggtgc agcagcaacg
     6601 tccgccacta cttcaacagc aaagagctcc gtttcccgga caaatgccca atctacccaa
     6661 gccagcgctc ttctggcagc aagaggcgca gaagcaggag gcgctgcagc aggaggcgca
     6721 gcagcaggag gcgcagaaga agcagcagca ggcgcatgcg caaatggagc ctgaacgtat
     6781 caatagccag catttctaca gaagcggcca gacaggagcg gcgttgaatc agccgccatt
     6841 gggtgctcca tcaaaaaggc agtggcatga gtgggtgcac ccgcgcatgc agcacgccaa
     6901 cgcattccac gctggcgtca acaatggata tcagatgcgt cccaaaaaga atatagctgc
     6961 gcagagcacc tttaacaaca acgtgcacat gcacctccag caaccatatt acccgaatca
     7021 gcagcagcag cagcagcagc agcagccact ccagctgacg gagatcaaca atgcgccacc
     7081 gcgatactca acgatccaag attttgtgcc catacaggcg taccgtccca agaaattaaa
     7141 tcgcgtccaa ccagccggtc gtcaggacgt cgatgccacc aagaatccta gtcgttcgcc
     7201 tgtgttgcag caaccgacga acgagactat agacacaaag gccagttcta gtcttccggt
     7261 acaatcagca ggcgaacagg tgattggact gatgcaaacg cttgaaatta agccggctgc
     7321 gtcgcagtcc gaatctgatg gggtctcaac aggcagtgcg aacgcgccca cagcaacgac
     7381 atcatcccag gcggtcaacc gaaggaagca tcgagtgcct cgcatcggag ccaagttcga
     7441 cttggaatac atactgcccg actccccgca tccaacttct agagggccct tcgaaggtaa
     7501 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca
     7561 ccattgagtt taaacccgct gatcagcctc gactgtgcct tctaaggcct gagctcgctg
     7621 atcagcctcg atcgaggatc cagacatgat aagatacatt gatgagtttg gacaaaccac
     7681 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     7741 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     7801 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     7861 tatggctgat tatgatcagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     7921 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     7981 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     8041 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     8101 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     8161 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     8221 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     8281 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     8341 ttaccagcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8401 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8461 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8521 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8581 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8641 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8701 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8761 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8821 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8881 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8941 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9001 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9061 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9121 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9181 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9241 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9301 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9361 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9421 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     9481 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9541 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9601 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9661 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9721 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     9781 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9841 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9901 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9961 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10021 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10081 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10141 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    10201 ggccctttcg t
//
