LOCUS       sequence-289720-       10397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmeIF4Ga-del205-234-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10397)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 10397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4Ga-del205-234-dsRNAres_Q
FEATURES             Location/Qualifiers
     source          1..10397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7682..7723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7733..7750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7773..7790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7823..7957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7847..7866)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7901..7920
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8579..9167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8659..8678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9338..10129,10130..10198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9961..9980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10199..10303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10371..10389
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgca tgcagcaagc
     2761 aatccctaca ctacctactc agtcggacat cgacaaggct atgcaaccac actcggctca
     2821 gaacatgtac gtgtctacag gtaataataa ctcaggtaac acgcggtcaa acccgcagag
     2881 cggtgggatt ttccgtgggc ctccatctac accaaacgct ccaaggggag caagcggagg
     2941 ggctacgcga cacgtgcacg ttcagccaat gtattcgcag ccattgcatc aaaatatggt
     3001 tctccagcag tatacgcagt acaatcctcg acagcagacc ttcccggcat cacacttaca
     3061 gtatgccccg gctccgatgc cctattatca atatcagtac gtgcccacat tgcaacagca
     3121 acctcctcac acacggagcg ccgtgactgt gaacactaat gtgaacgtag ggaataacct
     3181 ccaacccgtt caatcaggcc caaacggccc attgccagtc ccaggcgcca gttcttctca
     3241 gatacagctt ttgacgagta cggtacaacc tggtgctagt acagtgatgg gagtgggagg
     3301 accaggcagc acgatgggac aggtaggagt cccccccatg gtcggtgtgg gagtgatgac
     3361 taaaaatatt ttagacgatt ttgataagac aaaatcaaac acagacaatg aattttccga
     3421 tcaggtaacc tccacaaaca caccagcaac tgtcctttca gaaggtccaa tacgtattcc
     3481 acagcaagaa agtgttggtt taaataattt gaccagtaca tcatcacaag gatcagaatc
     3541 acggacaaac gcgccttata tccccattga aacaacacca ataagtcgaa cagatgttgg
     3601 gccgactcca atcgtatccg cgatgactga tgctccttcg gttgaaatat taccaacgcc
     3661 ccaaagaggg agaagcagaa aaattcctat agtgtctcct aaaaatgtat ctgagtccat
     3721 agttgctcct atcactgatg aaacggatga tgcagggtca acaccgatat ccagagccac
     3781 tgaagaatct atgccaccta atcagaccca tccaaattta ctcatatccg attcatcaaa
     3841 acataagcaa gcagtatcaa atagtgagat ttcaaaagac gctcctactg gtttaaagga
     3901 aatgcatgtt gctgagctgg agtccagtgt agcctccgaa aatcatccag ctggaatact
     3961 agatgtcaat gtcaacaaga gttcacaatc attggatttt tctgcagatc ccgaaattga
     4021 tagtattggc gcaatatcac ctacgagtcc aaacgcagtt tctcctattt tacacgaagt
     4081 tttagataat actgagttgt caaaaaaatt agagaattct acgacagaaa gatttaaaga
     4141 tagccaaagc gtagagaagc caactcatca agaactaagt ttaagaaatg ccacagacga
     4201 gactgaaata tccgcaatgg cattacaaga tgtgaatagt ttggataata accaaattga
     4261 aaaaacatac ccttcaaagc cgaaactcaa tgtagatgtt tcagaagaca tttcctccag
     4321 agagtcagca ataaagtcaa cttctacaaa aaatacagga gtagatgtag gtctccaatc
     4381 agattctaag ccagagacta cccttaacga taaacaggac tctactgacc taaaagtgaa
     4441 agtgtctgcg aaaatttcat ctatcataaa ctataatgag ggtcaatggt caccaaacaa
     4501 tccaagcgga aaaaaacaat acgaccgaga acagttatta cagttacgcg aggttaaggc
     4561 ctcacgtata caaccagagg taaaaaatgt gtctattctt cctcaaccaa acttaatgcc
     4621 atcatttatt cgaaataaca ataataacaa gcgagtacaa tcgatggttg gtattattgg
     4681 caatcgtagc aatgaatcgg cgggaaatta tattggtaaa cagatgtcga tgtctggcgt
     4741 acaaagtggc ggcggtagaa gcagtatgaa gggcatgata cacgtaaatt tatcattaaa
     4801 tcaagatgta aagttaagtg aaaatgaaaa tgcatggcgc cctcgggtct taaataaatc
     4861 tgatggtgat agcgacgcga aatctgcact cgaaaaagat gaattggtac gtcgtgttcg
     4921 gggtatttta aataagctta ccccagaaag gtttgacaca cttgttgagg agattataaa
     4981 attgaaaatt gatacacccg acaaagtgga cgaagtaatt gtgttggtat ttgaaaaggc
     5041 cattgatgag ccaaactttt ctgtgtctta cgccagacta tgtcaacgac ttgctgcgga
     5101 agtgaaggta atagacgagc gcatggaaag cgaaaccaaa tctaactcgg cacactttcg
     5161 aaatgcattg ttagataaaa ctgaacagga attcacccaa aacgtttcac aaagcaccgc
     5221 aaaggaaaaa aaattacagc caattgtgga taaaattaag aaatgtactg atgccaatga
     5281 aaaagcggaa cttgaggcat ttttggaaga agaagaaaga aaaataagaa gacgatccgg
     5341 agggacagta cgatttattg gcgaactttt taagatatct atgttgactg gcaaaataat
     5401 atactcttgc atcgatacac ttcttaaccc tcattctgaa gacatgcttg agtgtttgtg
     5461 caagctgctt acaactgtgg gtgctaaatt tgaaaagacc ccagtaaatt ccaaagaccc
     5521 tagtcggtgt tattcgttgg aaaaatcgat caccaggatg caggccatcg catcgaaaac
     5581 tgacaaagat ggagccagag taagctcaag agtacgattt atgcttcaag acgttatcga
     5641 cttgcgtaag aataaatggc aaacttcgcg gaatgaagca cccaaaacta tgggacaaat
     5701 tgaaaaagag gcaaagaatg agcagctttc tgcacaatat tttggtacac tttccagtac
     5761 tacacccgga ggatcgcagg gcggctctgg taaacgagat gaccgtggaa actctcgata
     5821 cggtgaatct cgttcaagta gcgcgtatgg tggcagccac agtcaacgtg gtgataatgg
     5881 caaccttcga caccagcagc agaacaacgt cgggggaaac gtctctggag gtgctggaca
     5941 ctccaatgga aacaatgatg aaaatacttg gcatgtgcaa accagcaaag gtagccgctc
     6001 tctagcggtt gacagcaata aactggaagg cctgtctaaa ctttctgacc aaaatctgga
     6061 aacaaagaag atgggtggat taacgcaatt catttggatt agtagtgaca cgacaagact
     6121 atcgtcggcg ccgacaccaa ccccatccaa tcctttcgct gtgctctcat ccctaattga
     6181 taaaaatagt aatgaaaggg atcgcgatcg cagtggaccc agaaacaaag gatcatacaa
     6241 caaaggttct atggagcggg atcgttatga tagaggtatg cattcgagaa ctggatcatc
     6301 tcagggatca cgagaaaact catcctcccg cggtggtcag cagggtcgaa ctttattgag
     6361 ttcatcagtt caaaaatcta ctagtcattc aaaatacacc caacaggcac cacctacacg
     6421 gcatactgtt aaagcccagt catcagtggg cagctctaac gttaacacag gtccactcta
     6481 ccgtggaagt gagcagcaaa catcagcaac cttctcgcag accacccgtt ctgttgctcc
     6541 tgtagcagtg ttcatcgaag ctagcgaaac agatttaaaa ctcatcaaat ctgttgtttc
     6601 agaaattgta gacctatcag ctgcatctaa agaagtcacg cccggtgcag tttcgtgcat
     6661 aaaacgagta ccagagaaac tacggtgcag tttcatttat tatattttaa cagattattt
     6721 gcatttagca aatgtaggta aacagtacag gcggtattta tctattgccg tctcccaact
     6781 tattcagcag aattacattt ctgccgatca tttgagactg gcttacaacg aatttaccgt
     6841 atatgcaaac gatttgattg ttgatattcc ggagctttgg ctatacattc ttcaatttgc
     6901 cgggccacta attgtaaaga aaatattgac tatatcagat ttgtggaaca acaatttaaa
     6961 agaaaatagt ccatcaaacg tcgctaaaaa gttccttaaa acatacttga tatattgcac
     7021 acaagaagtt ggaccaaatt ttgctcggaa tatgtggatc aagtttaacc taaaatggtc
     7081 agattttatg cctgaaagtg aagttgccga ctttattaaa tttaacagac tggaatatgt
     7141 ggaaaacgaa tcaaagtcgc cagtgattga tcatagagag actccagaaa agcacgttaa
     7201 aaatgtgata gaccatattg agcacttact aaaggaagga acaacggcgg actgcattat
     7261 tgattatagt aacggaaata taatggtggt tgataagttg tttatcagag gattgacgga
     7321 aacattgagt aatttctcga ttcactacaa ggacaacagt tacaagcttg agtccgaaac
     7381 ctttcaaaag ttttgcatac cggttttgca gagatacatt gattctaatg aagaccacca
     7441 acttgaatgt ttatatactt tgcagctttt agttcatgga ttggagcatc caagagggtt
     7501 gctgagtgaa ctaattggcg agctatatga tgcctttgta atacagaagg aatcgctttg
     7561 taagtggcga gattcaaagg atcagtctgc tggcaagggc gttgctgtaa aaagtcttaa
     7621 tccttttttt aactcgttgt taaacgatga tgccaaccct ctagagggcc cgcggttcga
     7681 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca
     7741 ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta aggcctgagc
     7801 tcgctgatca gcctcgatcg aggatccaga catgataaga tacattgatg agtttggaca
     7861 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7921 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7981 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     8041 atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc aacccagtca
     8101 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     8161 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     8221 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     8281 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     8341 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     8401 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     8461 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     8521 cggctcttac cagccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8581 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8641 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8701 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8761 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     8821 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     8881 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     8941 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9001 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9061 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9121 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9181 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9241 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9301 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9361 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9421 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9481 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9541 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9601 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     9661 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9721 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9781 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     9841 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     9901 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     9961 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10021 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10081 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10141 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10201 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10261 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10321 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10381 tcacgaggcc ctttcgt
//
