LOCUS       sequence-28975-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, 
            Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K
  TITLE     Tipin-replication protein A interaction mediates Chk1 
            phosphorylation by ATR in response to genotoxic stress.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16562-71. doi: 
            10.1074/jbc.M110.110304. Epub 2010 Mar 15.
  PUBMED    20233725
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.110304"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16562-71"
COMMENT     Sequence Label: pcDNA3-Flag-Tipin-E185A
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             115..138
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gggcnttagc gagctctctg gctactagag aacccactgc ttactggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctt ggtaccgagc tcggatccag gatggactac
      121 aaggacgacg atgacaagct agaaccacag gagaatggcg tgatcgacct accagattat
      181 gagcatgtag aagatgaaac ttttcctcct ttcccacctc cagcctctcc agagagacaa
      241 gatggtgaag gaactgagcc tgatgaagag tcaggaaatg gagcacctgt tcctgtacct
      301 ccaaagagaa cagttaaaag aaatataccc aagctggatg ctcagagatt aatttcagag
      361 agaggacttc cagccttaag gcatgtattt gataaggcaa aattcaaagg taaaggtcat
      421 gaggctgaag acttgaagat gctaatcaga cacatggagc actgggcaca taggctattc
      481 cctaaactgc agtttgagga ttttattgac agagttgaat acctgggaag taaaaaggaa
      541 gttcagacct gtttaaaacg aattcgactt gatctcccta ttttacatga agattttgtt
      601 agcaataatg atgaagttgc ggagaataat gaacatgatg tcacttctac tgaattagat
      661 ccctttctga caaacttatc tgaaagtgcg atgtttgctt ctgagttaag tagaagccta
      721 acagaagagc accaccaaga attgagagaa taac
//
