LOCUS       sequence-28976-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, 
            Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K
  TITLE     Tipin-replication protein A interaction mediates Chk1 
            phosphorylation by ATR in response to genotoxic stress.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16562-71. doi: 
            10.1074/jbc.M110.110304. Epub 2010 Mar 15.
  PUBMED    20233725
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.110304"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16562-71"
COMMENT     Sequence Label: pcDNA3-Flag-Tipin-E190A
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggagcatta gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc aggatggact
      121 acaaggacga cgatgacaag ctagaaccac aggagaatgg cgtgatcgac ctaccagatt
      181 atgagcatgt agaagatgaa acttttcctc ctttcccacc tccagcctct ccagagagac
      241 aagatggtga aggaactgag cctgatgaag agtcaggaaa tggagcacct gttcctgtac
      301 ctccaaagag aacagttaaa agaaatatac ccaagctgga tgctcagaga ttaatttcag
      361 agagaggact tccagcctta aggcatgtat ttgataaggc aaaattcaaa ggtaaaggtc
      421 atgaggctga agacttgaag atgctaatca gacacatgga gcactgggca cataggctat
      481 tccctaaact gcagtttgag gattttattg acagagttga atacctggga agtaaaaagg
      541 aagttcagac ctgtttaaaa cgaattcgac ttgatctccc tattttacat gaagattttg
      601 ttagcaataa tgatgaagtt gcggagaata atgaacatga tgtcacttct actgaattag
      661 atccctttct gacaaactta tctgaaagtg agatgtttgc ttctgcgtta agtagaagcc
      721 taacagaaga gcacca
//
