LOCUS       sequence-289769-        5744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of DmNot10_407-635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5744)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pnYC-NpG-DmNot10_407-635_Q
FEATURES             Location/Qualifiers
     source          1..5744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2294..2313
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2402..2421
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2402..2420
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2421..2445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2460..2482
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2489..3142
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             3164..3187
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(3976..3994)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3990..4037
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4212..4217,4218..4234,4235..4240))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      4352..4897
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4798..4817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5014..5031
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        5423..5525
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(5526..5744,1..441)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFGMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5592..5611)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ggtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
       61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
      121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
      181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
      241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
      301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
      361 gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
      421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
      481 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
      541 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
      601 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
      661 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
      721 gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc
      781 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg
      841 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg
      901 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
      961 cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg
     1021 ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt
     1081 tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg
     1141 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     1201 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     1261 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
     1321 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     1381 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     1441 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     1501 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     1561 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
     1621 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     1681 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     1741 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     1801 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     1861 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     1921 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     1981 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     2041 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     2101 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     2161 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     2221 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     2281 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     2341 tgatgccggc cacgatgcgt ccggcgtaga ggatcagact aagatctcga tcccgcgaaa
     2401 ttaatacgac tcactatagg ggaattgtga gcggataaca attccccact agtaataatt
     2461 ttgtttaact ttaagaagga gatataccat ggcccctata ctaggttatt ggaaaattaa
     2521 gggccttgtg caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca
     2581 tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga
     2641 gtttcccaat cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat
     2701 catacgttat atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga
     2761 gatttcaatg cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata
     2821 tagtaaagac tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa
     2881 aatgttcgaa gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc
     2941 tgacttcatg ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga
     3001 tgcgttccca aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa
     3061 gtacttgaaa tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg
     3121 tggtggcgac catccgccaa aatcggatgg taccggcagc ggcctggaag ttctgttcca
     3181 ggggccccat atgctcgagc ttaagcaatt gggatccaca aagccatacg ctcctcaatc
     3241 ggcgggagtt ccagagccca cgctggaatt cgctgtcttg tgtctacgta gcgcgttaac
     3301 gcttacgcag cactacaaga cgaggtttca catggcggcg gtctcctcgg aggatgtgga
     3361 agcacccgaa cccaaggacc caactcagga gagttggcgt catccccagg acaataactt
     3421 ctgcaatccc tcgaagccag tcagcctaga gtctttggaa aacatgatgg ctgccatata
     3481 tgctgctcac agcttcgtct cgctgcgcct aggcgatcat gtgaccgcgc tcgagatgtc
     3541 ggaaaaactg ctggcctgtg aacgcttgtc cgatgcacac aaattgctag gtcacatgta
     3601 cgctggcgag gcgttgatgc ttatggacaa ggccagcgag gcacgcgacc atttggaccc
     3661 tacttttgtg ggcacactaa acgccttcga ttttgagacg cgtgactggc aactgaagtc
     3721 ggtggacgcc gctcagaacg ttgtgcggta taatctggcc gttgccatgg ctctacagaa
     3781 cgacctgcct caagctaaag cactcctggc caatttaact cattcgctgg ttgccaacaa
     3841 ggcgcttgct ttgcgacgct tcatggacct caaaatgggt cccgttcctg gcgggacgcc
     3901 gagttagtct agagctagcc ctaggagatc cggctgctaa caaagcccga aaggaagctg
     3961 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     4021 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg
     4081 cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac
     4141 gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga
     4201 taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tacaacttat
     4261 atcgtatggg gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatcaaga
     4321 aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct
     4381 cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta
     4441 ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt
     4501 cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg
     4561 gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg
     4621 gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     4681 actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg
     4741 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccaggggg
     4801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     4861 ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc
     4921 acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc
     4981 atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     5041 tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat
     5101 gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata cactccccta
     5161 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
     5221 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     5281 tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag
     5341 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     5401 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     5461 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     5521 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     5581 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     5641 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     5701 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attc
//
