LOCUS       sequence-289783-        6945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6C_1207-1690-CIM2mut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsTNRC6C_1207-1690-CIM2mut_Q
FEATURES             Location/Qualifiers
     source          1..6945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4230..4271
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4281..4298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4321..4338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4371..4505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4395..4414)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4449..4468
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5127..5715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5207..5226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5886..6677,6678..6746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6509..6528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6747..6851)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6919..6937
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat ccagcacagt ggcggccgcg cccaggccct
     2761 gctcgtgaag cagccaccac cgccaccgcc cccgccgcac ctgtctctgc acccctctgc
     2821 aggcaaatcg gccatggaca gcttcccctc gcacccacag actcccggcc tacctgacct
     2881 gcagaccaaa gagcagcagt cttcacccaa cacctttgct ccttaccctc tcgctggact
     2941 gaacccaaac atgaatgtca acagcatgga catgaccggt ggcttgtcgg tgaaggaccc
     3001 atcccagtcc cagtcacgcc tcccccagtg gacgcacccc aactccatgg ataacttgcc
     3061 cagtgccgct tcccccctgg agcagaaccc tagcaagcat ggtgctatcc ctggaggtct
     3121 aagcattggg cctccaggta agtcctccat tgatgactcc tatggccggt acgatttaat
     3181 ccagaacagt gagtcaccag ccagtcctcc cgtagctgtt ccccatagct ggtcacgtgc
     3241 caaatctgac agtgataaaa tctcaaatgg ctctagcatc aactggcccc cagaattcca
     3301 tccgggagtt ccatggaaag gactgcagaa tattgaccct gagaatgacc ctgacgtcac
     3361 tcctggcagt gtccccactg ggcctaccat caacaccacc atccaggatg tcaaccgcta
     3421 cctcctcaag agtggaggta aactgtcaga catcaaatcg acgtggtcct ctggccctac
     3481 ctcccacacg caagcctctc tgtctcatga actatggaag gtgcccagaa acagtactgc
     3541 acccacgagg ccacctccag ggttaaccaa tcccaagccc tcctccacct ggggtgccag
     3601 ccccctcggc tggaccagct cctactcctc gggttctgcc tggagcaccg acacctcagg
     3661 aagaaccagc agctggctcg ttcttcgaaa cctcactccc cagattgatg gttctacact
     3721 gcggacattg tgtttgcaac atgggcctct tatcacattc cacctgaatc tgactcaagg
     3781 caatgctgtg gtccggtaca gctccaagga ggaggctgcc aaggcccaga agtctctgca
     3841 catgtgcgtc ctgggaaaca ctaccatcct ggccgagttc gctggtgaag aagaagtgaa
     3901 tcgcttctta gcccaaggcc aggcgctgcc acccacttcc agctggcagt ccagcagcgc
     3961 gtccagccag ccgcggctca gcgcagcggg cagctcccat ggcctggtac gcagcgacgc
     4021 tggccactgg aacgccccgt gcctgggtgg caaggggagc agtgagctgg cggcggccgg
     4081 ggtgccccag tactccagca gcgcggcggc cccgcccagc gccgacgaca gcagggtgat
     4141 aggcagcccc acgccgctaa ccaccctgct gcctggggac ctgctcagcg gggagtccct
     4201 gtagtaatct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
     4261 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     4321 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca
     4381 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4441 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4501 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4561 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg
     4621 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     4681 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     4741 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     4801 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     4861 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     4921 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     4981 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     5041 catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac
     5101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     5341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6181 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6481 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     6541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6841 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6901 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
