LOCUS       sequence-28981-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, 
            Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K
  TITLE     Tipin-replication protein A interaction mediates Chk1 
            phosphorylation by ATR in response to genotoxic stress.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16562-71. doi: 
            10.1074/jbc.M110.110304. Epub 2010 Mar 15.
  PUBMED    20233725
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.110304"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16562-71"
COMMENT     Sequence Label: pcDNA5/FRT/TO-Flag-Tipin-L195A
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             206..229
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggggcattag cgagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccgag ctcggatcca ggatggacta caaggacgac gatgacaagc tagaaccaca
      241 ggagaatggc gtgatcgacc taccagatta tgagcatgta gaagatgaaa cttttcctcc
      301 tttcccacct ccagcctctc cagagagaca agatggtgaa ggaactgagc ctgatgaaga
      361 gtcaggaaat ggagcacctg ttcctgtacc tccaaagaga acagttaaaa gaaatatacc
      421 caagctggat gctcagagat taatttcaga gagaggactt ccagccttaa ggcatgtatt
      481 tgataaggca aaattcaaag gtaaaggtca tgaggctgaa gacttgaaga tgctaatcag
      541 acacatggag cactgggcac ataggctatt ccctaaactg cagtttgagg attttattga
      601 cagagttgaa tacctgggaa gtaaaaagga agttcagacc tgtttaaaac gaattcgact
      661 tgatctccct attttacatg aagattttgt tagcaataat gatgaagttg cggagaataa
      721 tgaacat
//
