LOCUS       sequence-289818-       11871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-4xmut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Petit AP, Wohlbold L, Bawankar P, Huntzinger E, Schmidt S, 
            Izaurralde E, Weichenrieder O
  TITLE     The structural basis for the interaction between the CAF1 nuclease 
            and the NOT1 scaffold of the human CCR4-NOT deadenylase complex.
  JOURNAL   Nucleic Acids Res. 2012 Nov;40(21):11058-72. doi: 
            10.1093/nar/gks883. Epub 2012 Sep 12.
  PUBMED    22977175
REFERENCE   2  (bases 1 to 11871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks883"; journalName: "Nucleic Acids Res"; date: 
            "2012-11"; volume: "40"; issue: "21"; pages: "11058-72"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1-4x mut_R
FEATURES             Location/Qualifiers
     source          1..11871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8659..8780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8696..8715)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8750..8769
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8787..9242)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8924..8945)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9136..9155
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9269..9373
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9375..9732
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9375..9566
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9398..9418)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9583..9718
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9767..10561
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9767..10561
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9821..9840)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10431..10450
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10749..10768)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10793..10840
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11169..11757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11658..11677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcattc aacattccct
     2641 tataaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagacgga cttgtctcag gtgtggccag aggcaaacca
     3901 gcactttagt aaagagatag atgatgaagc aaacagctat ttccagcgaa tatataatca
     3961 tccaccacat ccaaccatgt ctgttgatga ggtattagaa atgctgcaga gatttaaaga
     4021 ctctactata aagagggaac gagaagtatt taactgtatg ctaaggaact tgtttgaaga
     4081 atatcgtttt tttccccagt atcctgataa agagttacat ataacagcct gcctatttgg
     4141 tggtataatt gagaaaggac tggtcactta catggcacta ggtctggctc tacgatatgt
     4201 tcttgaagcc ttacgcaagc cttttggatc caaaatgtat tatttcggga ttgctgcact
     4261 agatagattt aaaaacagat tgaaggacta tccccagtat tgtcagcatt tggcttctat
     4321 cagtcacttt atgcaatttc cacatcattt acaggagtat attgagtatg gacagcagtc
     4381 tagagatcct cctgtgaaaa tgcaaggctc tatcacaacc cctggaagta ttgcactggc
     4441 tcaggcccag gctcaggccc aggttccagc aaaagctcct cttgctggtc aagttagcac
     4501 tatggtaacc acctcaacaa ctaccactgt tgctaaaacg gttacggtca ccaggccaac
     4561 tggagtcagc tttaagaaag atgtgccacc ttctattaat actacaaata tagatacgtt
     4621 gcttgtggcc acagatcaaa ctgagagaat tgtggagccc ccagaaaata tccaggagaa
     4681 aattgctttt attttcaata atctctcaca gtcaaatatg acacaaaagg ttgaagagct
     4741 aaaggaaacg gtgaaagaag aatttatgcc ttgggtttca cagtatctgg ttatgaagag
     4801 agtcagtatt gagccaaact ttcatagcct gtattcaaac ttccttgaca cgctgaagaa
     4861 tcctgaattt aacaagatgg ttctgaatga gacctacaga aacattaaag tgctcctgac
     4921 ctctgataaa gctgcagcca atttctcaga tcgttctttg ctgaagaact tgggacattg
     4981 gctaggaatg atcacattag ctaaaaacgc acccatctta gccactgact tggatgtggc
     5041 atcattgctg ctagaggctt atgttaaagg acaacaagaa ttgctctatg tagtgccctt
     5101 tgttgccaaa gtcttagaat ctagcattcg tagtgtgcgt tttaggccac caaacccttg
     5161 gacaatggca attatgaatg tattagctga gctacatcag gagcatgact taaagttaaa
     5221 cttgaagttt gaaatcgagg ttctctgcaa gaaccttgca ttagacatca atgagctaaa
     5281 acctggaaac ctcctaaagg ataaagatcg cctgaagaat ttagatgagc aactctctgc
     5341 tccaaagaaa gatgtcaagc agccagaaga actccctccc atcacaacca caacaacttc
     5401 tactacacca gctaccaaca ccacttgtac agccacggtt ccaccacagc cacagtacag
     5461 ctaccacgac atcaatgtct attcccttgc gggcttggca ccacacatta ctctaaatcc
     5521 aacaattccc ttgtttcagg cccatccaca gttgaagcag tgtgtgcgtc aggcaattga
     5581 acgggctgtc caggagctgg tccatcctgt ggtggatcga tcaattaaga ttgccatgac
     5641 tacttgtgag caaatagtca ggaaggattt tgccctggat tcggaggaat ctcgaatgcg
     5701 aatagcagct catcacatga tgcgtaactt gacagctgga atggctatga ttacatgcag
     5761 ggaacctttg ctcatgagca tatctaccaa cttaaaaaac agttttgcct cagcccttcg
     5821 tactgcttcc ccacaacaaa gagaaatgat ggatcaggca gctgctcaat tagctcagga
     5881 caattgtgag ttggcttgct gttttattca gaagactgca gtagaaaaag caggccctga
     5941 gatggacaag agattagcaa ctgaatttga gctgagaaaa catgctaggc aagaaggacg
     6001 cagatactgt gatcctgttg ttttaacata tcaagctgaa cggatgccag agcaaatcag
     6061 gctgaaagtt ggtggtgtgg acccaaagca gttggctgtt tacgaagagt ttgcacgcaa
     6121 tgttcctggc ttcttaccta caaatgactt aagtcagccc acgggatttt tagcccagcc
     6181 catgaagcaa gcttgggcaa cagatgatgt agctcagatt tatgataagt gtattacaga
     6241 actggagcaa catctacatg ccatcccacc aactttggcc atgaaccctc aagctcaggc
     6301 tcttcgaagt ctcttggagg ttgtagtttt atctcgaaac tctcgggatg ccatagctgc
     6361 tcttggattg ctccaaaagg ctgtagaggg cttactagat gccacaagtg gtgctgatgc
     6421 tgaccttctg ctgcgctaca gggaatgcca cctcttggtc ctaaaagctc tgcaggatgg
     6481 ccgggcatat gggtctccat ggtgcaacaa acagatcaca aggtgcctaa ttgaatgtcg
     6541 agatgaatat aaatataatg tggaggctgt ggagctgcta attcgcaatc atttggttaa
     6601 tatgcagcag tatgatcttc acctagcgca gtcaatggag aatggcttaa actacatggc
     6661 tgtggcattt gctatgcagt tagtaaaaat cctgctggtg gatgaaagga gtgttgctca
     6721 tgttactgag gcagatctgt tccacaccat tgaaaccctc atgaggatta atgctcattc
     6781 cagaggcaat gctccagaag gattgcccca gctgatggaa gtagtgcgat ccaactatga
     6841 agcaatgatt gatcgtgctc atggaggccc aaactttatg atgcattctg ggatctctca
     6901 agcctcagag tatgatgacc ctccaggcct gagggagaag gcagagtatc ttctgaggga
     6961 atgggtgaat ctctaccatt cagcagcagc tggccgcgac agtaccaaag ctttctctgc
     7021 atttgttgga cagatgcacc agcaaggaat actgaagacc gatgatctca taacaaggtt
     7081 ctttcgtctg tgtactgaaa tgtgtgttga aatcagttac cgtgctcagg ctgagcagca
     7141 gcacaatcct gctgccaatc ccaccatgat ccgagccaag tgctatcaca acctggatgc
     7201 ctttgttcga ctcattgcac tgctcgtgaa acactcaggg gaggccacca acactgtcac
     7261 aaagattaat ctgctgaaca aggtccttgg tatagtagtg ggagttctcc ttcaggatca
     7321 tgatgttcgt cagagtgaat ttcagcaact tccctaccat cgaattttta tcatgcttct
     7381 cttggaactc aatgcacctg agcatgtgtt ggaaaccatt aatttccaga cacttacagc
     7441 tttctgcaat acattccaca tcttgaggcc taccaaagct cctggctttg tatatgcctg
     7501 gcttgaactg atttcccatc ggatatttat tgcaagaatg ctggcacata cgccacagca
     7561 gaaggggtgg cctatgtatg cacagctact gattgattta ttcaaatatt tagcgccttt
     7621 ccttagaaat gtggaactca ccaaacctat gcaaatcctc tacaagggca ctttaagagt
     7681 gctgctggtt cttttgcatg atttcccaga gttcctttgt gattaccatt atgggttctg
     7741 tgatgtgatc ccacctaatt gtatccagtt aagaaatttg atcctgagtg cctttccaag
     7801 aaacatgagg ctccccgacc cattcactcc taatctaaag gtggacatgt tgagtgaaat
     7861 taacattgct ccccggattc tcaccaattt cactggagta atgccacctc agttcaaaaa
     7921 ggatttggat tcctatctta aaactcgatc accagtcact ttcctgtctg atctgcgcag
     7981 caacctacag gtatccaatg aacctgggaa tcgctacaac ctccagctca tcaatgcact
     8041 ggtgctctat gtcgggactc aggccattgc gcacatccac aacaagggca gcacaccttc
     8101 aatgagcacc atcactcact cagcacacat ggatatcttc cagaatttgg ctgtggactt
     8161 ggacactgag ggtcgctatc tctttttgaa tgcaattgca aatcagctcc ggtacccaaa
     8221 tagccacact cactacttca gttgcaccat gctgtacctt tttgcagagg ccaatacgga
     8281 agccatccaa gaacagatca caagagttct cttggaacgg ttgattgtaa ataggccaca
     8341 tccttggggt cttcttatta ccttcattga gctgattaaa aacccagcgt ttaagttctg
     8401 gaaccatgaa tttgtacact gtgccccaga aatcgaaaag ttattccagt cggtcgcaca
     8461 gtgctgcatg ggacagaagc aggcccagca agtaatggaa gggacaggtg ccagttagcc
     8521 gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     8581 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     8641 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     8701 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     8761 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
     8821 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     8881 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     8941 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     9001 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     9061 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     9121 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     9181 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     9241 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9301 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9361 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     9421 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     9481 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9541 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     9781 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     9841 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     9901 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
     9961 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
    10021 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
    10081 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
    10141 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
    10201 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
    10261 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
    10321 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
    10381 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
    10441 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
    10501 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
    10561 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
    10621 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
    10681 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
    10741 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
    10801 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
    10861 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
    10921 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
    10981 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
    11041 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    11101 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    11161 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    11221 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11281 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    11341 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11401 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11461 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11521 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11581 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11641 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11701 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11761 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11821 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
