LOCUS       sequence-28984-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, 
            Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K
  TITLE     Tipin-replication protein A interaction mediates Chk1 
            phosphorylation by ATR in response to genotoxic stress.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16562-71. doi: 
            10.1074/jbc.M110.110304. Epub 2010 Mar 15.
  PUBMED    20233725
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.110304"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16562-71"
COMMENT     Sequence Label: pFastBac-His-Flag-Tipin-L195A
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             146..166
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             185..208
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttnctgtttc gtacagtttt gtataaaaaa acctataaat attccggatt attcataccg
       61 tcccaccatc gggcgcggat ctcggtccga aaccatgtcg tactaccatc accatcacca
      121 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatccag
      181 gatggactac aaggacgacg atgacaagct agaaccacag gagaatggcg tgattgacct
      241 accagattat gagcatgtag aagatgaaac ttttcctcct ttcccacctc cagcctctcc
      301 agagagacaa gatggtgaag gaactgagtc tgatgaagag tcaggaaatg gagcacctgt
      361 tcctgtacct ccaaagagaa cagttaaaag aaatataccc aagctggatg ctcagagatt
      421 aatttcagag agaggacttc cagccttaag gcatgtattt gataaggcaa aattcaaagg
      481 taaaggtcat gaggctgaag acttgaagat gctaatcaga cacatggagc actgggcaca
      541 taggctattc cctaaactgc agtttgagga ttttattgac agagttgaat acctgggaag
      601 taaaaaggaa gttcagacct gtttaaaacg aattcgactt gatctcccta ttttacatga
      661 agattttgtt agcaataatg atgaagttgc ggagaataat gaacatgatg tcacttctac
      721 tgattagatc ctttctgacn cngttc
//
