LOCUS       sequence-289856-        8764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-del205-490-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, 
            Izaurralde E
  TITLE     The interactions of GW182 proteins with PABP and deadenylases are 
            required for both translational repression and degradation of miRNA 
            targets.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. 
            Epub 2012 Nov 21.
  PUBMED    23172285
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks1078"; journalName: "Nucleic Acids Res"; date: 
            "2013-01"; volume: "41"; issue: "2"; pages: "978-94"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-del205-490-dsRNAres_R
FEATURES             Location/Qualifiers
     source          1..8764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6049..6090
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6100..6117
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6140..6157)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6190..6324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6214..6233)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6268..6287
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6946..7534)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7026..7045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7705..8496,8497..8565))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8328..8347
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8566..8670)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8738..8756
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatgc gtgaagccct tttttcccaa gatggctggg gctgtcagca
     2761 tgttaaccag gatactaatt gggaagttcc cagttcgcca gaaccagcca ataaggatgc
     2821 acccggtcca ccaatgtgga agccaagcat taacaatggt actgatcttt gggagtccaa
     2881 tttgagaaac ggaggtcagc cggccgcaca gcaagttcca aagccgtcgt ggggtcatac
     2941 accatcctct aacttaggtg gaacatgggg tgaggacgac gatggcgccg atagtagtag
     3001 tgtgtggact ggaggagctg ttagcaacgc gggatccgga gctgcagtgg gagtaaacca
     3061 agccggagtt aatgtcggtc caggcggtgt tgtttcgtct ggcggacctc agtggggaca
     3121 aggtgtcgtt ggcgtcggac ttggatcaac tggcggaaat ggttccagta acattacagg
     3181 ctcttcgggc gttgccacgg gaagcagtgg caattcaagt aatgccggaa atggatgggg
     3241 cgatccacgc gagattcgtc cactgggcgt cggaggatcg atggacatcc gtaacgtcga
     3301 gcaccgtgga ggaaatggat caggcgcccc gccaagcaat agtgcttcat caggttggag
     3361 cactgctgcg ggcgtggtgg atggggttga tctaggatct gagtggaaca cgcacggggg
     3421 gattattgga aaatctcagc aacaacaaaa actagcggga cttaacgtgg gaatggtgaa
     3481 cgtaattaac gcggagatca ttaagcaaag caagcaatac aggatccttg tcgagaacgg
     3541 ctttaaaaag gaagatgtag agcgggcatt agtgattgct aatatgaaca tcgaagaggc
     3601 agccgatatg ctccgtgcca actcatccct atcaatggat ggttggcgtc gacatgatga
     3661 gtcccttgga tcttatgccg accacaatag ttcaacaagc agcggtggat ttgctggtcg
     3721 ttacccggtc aacagtggac aaccttcaat gtcctttcct cataataacc ttatgaataa
     3781 catgggaggt accgctgtta ctggaggtaa caacaataca aacatgacag ctttacaggt
     3841 gcaaaagtat ttaaatcaag ggcaacatgg tgtcgctgtt ggaccgcaag ccgttggtaa
     3901 ttcttcagca gtatctgtcg gatttggtca gaacacgtct aacgcagcag tggcaggagc
     3961 agcctctgca aatatagcag caaatacaaa caaccaaccg tctggtcagc aaattcgcat
     4021 gctaggccag caaattcagt tggccattca tagtggtttc atatctagtc agatattgac
     4081 tcaaccgcta actcaaacaa cccttaacct tttaaaccaa cttcttagca atattaagca
     4141 tctccaggct gcacagcaat cccttacccg cgggggaaat gtcaatccaa tggcagtgaa
     4201 tgtggctata tctaaataca agcagcaaat ccagaattta cagaaccaga taagtgcaca
     4261 acaggctgtg tatgtaaaac agcaaaatat gcaaccaact tcacaacaac aacagcccca
     4321 acaacagcaa cttccttctg ttcatctaag taactcaggc aacgactatt taagaggtca
     4381 cgatgcaata aataatttgc aaagcaactt ttctgagctc aatattaata agccaagtgg
     4441 atatcaagga gcgtccaatc aacaatcccg attaaatcag tggaagcttc cagtattaga
     4501 taaggagatc aactctgaca gtacggaatt ttctcgtgcc ccaggtgcaa cgaaacaaaa
     4561 tttgacggcc aacacaagca acataaactc tttgggtctt caaaacgata gtacatggtc
     4621 aactggacgc agtattggtg acggttggcc tgatccctca tctgataacg agaataaaga
     4681 ctggtctgtt gctcagccaa cttcagcagc aactgcttac actgatctgg tccaagagtt
     4741 tgagccaggc aagccatgga agggttcaca gatcaaaagc atagaagatg atcccagcat
     4801 tacaccagga agcgttgcta gatctccatt gtctattaat tcgacgccaa aagatgctga
     4861 catatttgcc aataccggta aaaattcacc gactgattta ccgccactaa gtttatcgtc
     4921 gtctacatgg agttttaatc caaaccaaaa ttatccgagt cacagttggt ctgacaatag
     4981 tcaacaatgt accgccactt cggagctttg gacaagcccg ctaaataaat catcgtctcg
     5041 aggtcccccg ccaggattga ctgccaattc aaataaatct gcaaatagta atgcgtcaac
     5101 gccaacaact attaccggag gtgcgaatgg atggttacag cctcgaagtg gcggtgttca
     5161 aaccacaaac actaattgga caggtggtaa caccacttgg ggctccagtt ggttgctttt
     5221 gaaaaatcta acagcacaga ttgatggtcc tactttgcgt acactgtgta tgcagcatgg
     5281 gccccttgtc agctttcacc cgtatttgaa ccaaggaatt gccttatgta aatatactac
     5341 tcgtgaggag gcgaacaagg cgcaaatggc gttaaacaac tgtgtcctcg ccaacaccac
     5401 aatatttgct gaatctccca gcgagaacga ggtgcaaagc attatgcagc acttaccaca
     5461 aactccttcc tctacaagct ctagtggaac tagtggtggc aacgtcggag gcgtcggcac
     5521 ttcagccaat aatgcaaaca gtggttctgc agcttgtctg tccggaaaca atagcggcaa
     5581 cggaaacggc agcgcgagcg gcgccggcag cggcaacaat ggcaacagta gctgcaacaa
     5641 cagtgccgcc ggggggggca gcagcagcaa caacacgatt accactgtag caaattcgaa
     5701 tcttgttggt tctagtggct ctgtctcaaa ttcctctggc gttactgcta actctagtac
     5761 tgtttctgta gttagttgta cagcgagtgg gaattccata aatggggcag gtactgcaaa
     5821 cagttctggt tcaaagagta gtgcaaacaa tttagctagc ggccagtcta gcgcttctaa
     5881 cttaactaat agcaccaatt caacatggcg acaaactagc caaaaccaag ctcttcaaag
     5941 tcaaagcagg ccatcaggca gagaagctga ctttgattat atatctctcg tttattccat
     6001 tgttgatgat taagcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     6061 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
     6121 gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc
     6181 tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     6361 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     6421 gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     6481 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     6541 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     6601 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     6661 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     6721 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     6781 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     6841 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     6901 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6961 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7021 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7081 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7141 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7201 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7261 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7321 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7381 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7441 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7501 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7561 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7621 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7681 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7741 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7801 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7861 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7921 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7981 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     8041 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8101 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8161 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8221 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8281 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     8341 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8401 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8461 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8521 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8581 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8641 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8701 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8761 tcgt
//
