LOCUS       sequence-28986-e         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, 
            Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kacmaz K
  TITLE     Tipin-replication protein A interaction mediates Chk1 
            phosphorylation by ATR in response to genotoxic stress.
  JOURNAL   J Biol Chem. 2010 May 28;285(22):16562-71. doi: 
            10.1074/jbc.M110.110304. Epub 2010 Mar 15.
  PUBMED    20233725
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.110304"; journalName: "J Biol Chem"; date: 
            "2010-05-28- 28"; volume: "285"; issue: "22"; pages: "16562-71"
COMMENT     Sequence Label: pFastBac-His-Tipin-L195A
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             107..124
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             146..166
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttnctgtttc gtacagtttt gtataaaaaa acctataaat attccggatt attcataccg
       61 tcccaccatc gggcgcggat ctcggtccga aaccatgtcg tactaccatc accatcacca
      121 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatccgg
      181 aattcaaagg cctacgtcga cgagctcact agtcgcggcc gctttcgaat ctagactaga
      241 accacaggag aatggcgtga tcgacctacc agattatgag catgtagaag atgaaacttt
      301 tcctcctttc ccacctccag cctctccaga gagacaagat ggtgaaggaa ctgagcctga
      361 tgaagagtca ggaaatggag cacctgttcc tgtacctcca aagagaacag ttaaaagaaa
      421 tatacccaag ctggatgctc agagattaat ttcagagaga ggacttccag ccttaaggca
      481 tgtatttgat aaggcaaaat tcaaaggtaa aggtcatgag gctgaagact tgaagatgct
      541 aatcagacac atggagcact gggcacatag gctattccct aaactgcagt ttgaggattt
      601 tattgacaga gttgaatacc tgggaagtaa aaaggaagtt cagacctgtt taaaacgaat
      661 tcgacttgat ctccctattt tacatgaaga ttttgttagc aataatgatg aagttgcgga
      721 gaatatgaac atga
//
