LOCUS       sequence-289877-       10590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1-1709-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 10590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1-1709-dsRNAres_R
FEATURES             Location/Qualifiers
     source          1..10590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7875..7916
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7926..7943
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7966..7983)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8016..8150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8040..8059)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8094..8113
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8772..9360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8852..8871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9531..10322,10323..10391))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10154..10173
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10392..10496)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10564..10582
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta agatgaacgt agagagccaa ctgaagacac cgccaacgca
     2761 cattcgaaat ctggtcaagc acgtgaacaa gcgaaatttt aatgagagca gcgagcaaat
     2821 aaatcagttc gttaaagagc acggcctgga agcggatcgc agcagcctgc gccacctgtt
     2881 ctccgtgatc aacttcagcg atctcgtgcc atcagttacg gtccagctgc aggcgaagct
     2941 cctgggcatc cagttggagc gtcagcttca cagttcatcg tttgtgtcca acatctgcta
     3001 cgcttttgac cagttcttcg ccagcaacca gaagtccctg aagcctgttg cggtagcgga
     3061 tctcgttggc caggtcgccc gactgaccgg catcaacaag gtttgtgagt gcgtctttgc
     3121 cttggccttg acccactcct cgtacacaga actcaaacat tctgccagga acaacctaaa
     3181 aggtagcctc agcgaactta tagactctta cttgggtaat aaaggcaccg gcccagccga
     3241 cagcggtctc cgggagatct ccttcgatct actgcagtat ttgctgtgct gccttagcga
     3301 gtacgtacaa ccgcaggtgg aagcacagtt tttgatcaag cttcgtgagg agtttccgcg
     3361 ccaggcggta ccgctggttc tagcgccgtt cctttacggc tcgacgacgg cgacgattgc
     3421 gggagcaggt gccagcgaaa cggatgcgga ggccgaagcg acgactaata gcaacagctc
     3481 cagtttcgaa gcagatgctt tgaacgaggt gggaatcgag gatatttatg accatttaag
     3541 cgagataata ttcaccaacc agggcaaaaa taatattatg gacacatctt ggattaatct
     3601 aatccttgaa atcggttatg aatttacatc gagcgttgaa gagtgtaaga atcatttgtg
     3661 ttcccgcgag cgagaacgcg ctgagctcca gtccaaagat gtcgccaaga tcgtgggtct
     3721 tatgtgccga cggcactcct ccctgctcga ttgtaatgtg aatctaccga cgccggcgaa
     3781 cttctggcct ggtcagggac agggcggtgg cagcaacagc agtggctctt cgcagacaca
     3841 aatcactccg cagcaacaga acccgggcag cagtaacaac aacgatggca gcgatggcaa
     3901 ttcgtccagt gataagaagg acaagaagga gacgacggag gccacgcaaa cttggaagcc
     3961 agatgtcttt gtgcaggcgc tcaaggaggt ggtgcctcag ctcaactgga aggacgtgtg
     4021 catggaactt gatcatcccg agttcgtgct gaaggatcgc atcggtttgg agcttttgct
     4081 caccatcctt cggctggcca ctggctctaa catatttcca catccagaat gcatttaccg
     4141 tcactgggca aacacggaag gtcagctgtc gcttatcgcg acaatgctga agaacccgga
     4201 tctcttttcc ttcgccgact ttgtatttag ccagccagcg ttggatgtgc ttaagacagc
     4261 tccggatgcg gacaataagg agatctcgtc ctggaagtcg cttcacctgg tggaggtgct
     4321 gctctccatt gcggacaagg gatactatac gcaggtccat gagctattca agttcccagc
     4381 gcagaactgt cccgatgtgc tatttttggc cttattgaac acaagtccgc ctatgacgcc
     4441 actgcgtcag gatctgttta accaactgat accaacgttc cttggcaacc atcccaactc
     4501 gaacgtgatc ttggccagcg cctggagctc gaacagcttc caacttcgtt ccaatatcat
     4561 gaatgctatg tccgagtggt atctccgagg caatgagttc gatcaggtga aactgtcgcg
     4621 catcctggat cttgcccagg atttaaaagc gctatcagcg ctgctcaacg cccgttcatt
     4681 tttattcatt atcgacctgg cttgccttgc ctctcggcgc gagtacctga agctggaaaa
     4741 gtggcttacc gataaaattc gcgaacatgg agagcctttt atgcaggcta taatcaaggt
     4801 tctgcaccgc cgctgcccgc aggttatcaa cgccaaagtg ccagaggatc agctgcctcc
     4861 caagcaggcg cagcttttgc ccgagacagt tacaaccatg atcaactgtc tgcaaacgtg
     4921 catcaataac tgcatgcagc cggagatggt cgaagtcatc atgcaaatga ccgcgaacgt
     4981 ggcgattatg gctaacatgg cgcgtgccca acagcagcag cagccaggat tggttccgcc
     5041 gcctccacca acaattctgc gcggccatcg tggtatggat ctgcctggag gcatagtccc
     5101 tccgccaccg cagcaaccgt tctccggaaa cctcaacgcg cagatgtttg gaccaggaat
     5161 ggatccgcta acgaatatgt ccaacaactt agccggcctc aacctcagcg gcccgaacgg
     5221 agcatttaat tttggaaaca tgctaacttc tccatcgcgt ctgatgactc cgggagccaa
     5281 tccctatccg ctgaacctca tgcagatgcc gcaggctccg ccaccaccca acgtaggaaa
     5341 cctaggccgc atgctgccag ggggtccaca acagcagact ccaacgccga ctccaacggc
     5401 ccccaatcca aacaaccctg tgatggccga tctccagata cctgtgtcaa aggaagttga
     5461 agacgaggtc aactcatact ttcagcgcat ctacaaccac cagccgaatc caacgctgtc
     5521 catcgatgaa gtgctggaca ttttgcaaag atttaaagag tcaagtaacc ggcgcgagca
     5581 ggaagtcttt ttgtgcatgc tgcgcaatct gttcgaggaa tatcgctttt tctgtcaata
     5641 cccagagaaa gagctacaga ttactgcgca gctatttggt ggcattattg atcgtaacct
     5701 ggtgcccact tttgttgcac tgggcctttc tctgcgctgt gtcttggatg ccctccgcaa
     5761 gcccgatgga tctaagcttt actatttcgg tgtgacggca ctggacagat tcagaactcg
     5821 attgcacacc tacaacaaat actgcgagca tatccgctcc attcctcact tctctgactt
     5881 cccgccgcac cttattcagt atgtggaata cggaatgcac ggccaggagc cgccgccgca
     5941 gaagttgatt ggactcagca atacgattcc ttcggcgata tctagcggac caggaacgga
     6001 accaatttac aggaatagct ccatgttagg taatatgccc gctgccaccc cgggatcggg
     6061 accgaaatca aacgctgctg tatcgcatgc cacgagaatg aagtccatcg ccaatgccac
     6121 caatatcgac acacttttgg tggccaacca ggaagagaaa gtgactgtgc caccagagcc
     6181 tgtgcaggac aaaactgcct ttattttcaa caacttgagc cagctgaaca taccgcagaa
     6241 gtgcgatgag ataaaggaga ttatgaccaa ggagtattgg ccgtggctag cgcaatatct
     6301 ggtcctcaaa cgcgcatcaa tggagttcaa cttccacaca ctatattaca actttttgga
     6361 tgcccttaaa aacggcgaga tcaaccgatt cgtaaccaaa gagaccctac gcaacatcaa
     6421 ggtactcttg cgctccgata agggagttat taatttctcc gatcgaagtc tgttgaagaa
     6481 cctcggacac tggttgggca tgatgacctt aggtcgcaat cgccccatcc tgcagttgga
     6541 tctggatctt aaatcccttt tggctgaggc ttaccacaag ggccagcagg aactgctttt
     6601 tgtggttcct ttcgtagcca agatccttga gtcatccgcc aagtctcgca tcttccgatc
     6661 gcccaatcca tggacaatgg gtatcatgta cgtgcttgct gagcttcacc aggagccaga
     6721 ccttaagctg aacctgaagt ttgaaatcga agtactctgc aaaacactta atctggagtt
     6781 ggccaagttg cgacaagtga tctacctgaa ggatcccaat cgaactcacc ggattgaaga
     6841 gcagatgtcg caaccaaaac cgaaacaact tgagccagtg gcttctgcgc cagctctacc
     6901 gcgtgaacag caatccccgg cacaacctcc gccaccacca caacagcagc aaccaccgca
     6961 gcagcaggta cctccaccgc catcctctgc ggacgtggac gcccaaaatg cggccgctat
     7021 gatgatggca gcgggcggag ctaatagtac tcctggatcg gtatcttcgc ccaatctacc
     7081 caccgattcc agccaggtgg cactaccgcc accagagccg cgctattcat acgtggacgt
     7141 aaacgtgagc aacttccagc ttattggtca gcagttggta ctgccaccca atactccgtt
     7201 cctgcacgcc aatcccggaa tcaaacacat ggtcgtcaac gccgtggagc gcacgataac
     7261 cgactggttg cagcctattg tggaccgaag catccgcatt gcctgtgcca ccaccgagca
     7321 gatcatccgc aaggacttcg ccctggatgc tgacgagaac cgaatgcgca ctgccgctca
     7381 tcagatggta cgaaaccttg ccgccggcat ggccatgatt accggtaagg atgagatagc
     7441 gcgtgctatt agccagaatc tgcacaaggc cttgctgtcg ggtctaaatg ggatgcccag
     7501 catggctgag atccaagctg ctgctatgca gttagccagc gaaaatgttg agctagtttg
     7561 cgctttcatt cagaaaacat ctgctgaaaa ggcagccgca gagatcgata ggcgtctaag
     7621 caccgatttc gagaccagaa aaattgctcg tgaagagggc aaccgctttg tggacgccca
     7681 gatcctcacc tatcaacagg agcgtcttcc agaagcagtg cgcatcaagg tgggcgcggc
     7741 tccagctaca ctttatgctg tgtactcgga gttcgctaga agcatacctg gcttccagca
     7801 gatgagcgat cgcgatattg ccctgtttgt gcccaagccg acggatttgt gatctagagg
     7861 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     7921 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     7981 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     8041 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     8101 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     8161 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     8221 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     8281 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     8341 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     8401 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     8461 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     8521 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     8581 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     8641 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     8701 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     8761 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8821 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8881 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8941 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     9001 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9061 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9121 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9181 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9241 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9301 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9361 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9421 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9481 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9541 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9601 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9661 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9721 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9781 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9841 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9901 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9961 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10021 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10081 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10141 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10201 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10261 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10321 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10381 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10441 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10501 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10561 aaaaataggc gtatcacgag gccctttcgt
//
