LOCUS       sequence-289879-        9534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1_1149-2505-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R
FEATURES             Location/Qualifiers
     source          1..9534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6819..6860
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6870..6887
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6910..6927)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6960..7094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6984..7003)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7038..7057
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7716..8304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7796..7815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8475..9266,9267..9335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9098..9117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9336..9440)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9508..9526
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta aggtgccacc agagcctgtg caggacaaaa ctgcctttat
     2761 tttcaacaac ttgagccagc tgaacatacc gcagaagtgc gatgagataa aggagattat
     2821 gaccaaggag tattggccgt ggctagcgca atatctggtc ctcaaacgcg catcaatgga
     2881 gttcaacttc cacacactat attacaactt tttggatgcc cttaaaaacg gcgagatcaa
     2941 ccgattcgta accaaagaga ccctacgcaa catcaaggta ctcttgcgct ccgataaggg
     3001 agttattaat ttctccgatc gaagtctgtt gaagaacctc ggacactggt tgggcatgat
     3061 gaccttaggt cgcaatcgcc ccatcctgca gttggatctg gatcttaaat cccttttggc
     3121 tgaggcttac cacaagggcc agcaggaact gctttttgtg gttcctttcg tagccaagat
     3181 ccttgagtca tccgccaagt ctcgcatctt ccgatcgccc aatccatgga caatgggtat
     3241 catgtacgtg cttgctgagc ttcaccagga gccagacctt aagctgaacc tgaagtttga
     3301 aatcgaagta ctctgcaaaa cacttaatct ggagttggcc aagttgcgac aagtgatcta
     3361 cctgaaggat cccaatcgaa ctcaccggat tgaagagcag atgtcgcaac caaaaccgaa
     3421 acaacttgag ccagtggctt ctgcgccagc tctaccgcgt gaacagcaat ccccggcaca
     3481 acctccgcca ccaccacaac agcagcaacc accgcagcag caggtacctc caccgccatc
     3541 ctctgcggac gtggacgccc aaaatgcggc cgctatgatg atggcagcgg gcggagctaa
     3601 tagtactcct ggatcggtat cttcgcccaa tctacccacc gattccagcc aggtggcact
     3661 accgccacca gagccgcgct attcatacgt ggacgtaaac gtgagcaact tccagcttat
     3721 tggtcagcag ttggtactgc cacccaatac tccgttcctg cacgccaatc ccggaatcaa
     3781 acacatggtc gtcaacgccg tggagcgcac gataaccgac tggttgcagc ctattgtgga
     3841 ccgaagcatc cgcattgcct gtgccaccac cgagcagatc atccgcaagg acttcgccct
     3901 ggatgctgac gagaaccgaa tgcgcactgc cgctcatcag atggtacgaa accttgccgc
     3961 cggcatggcc atgattaccg gtaaggatga gatagcgcgt gctattagcc agaatctgca
     4021 caaggccttg ctgtcgggtc taaatgggat gcccagcatg gctgagatcc aagctgctgc
     4081 tatgcagtta gccagcgaaa atgttgagct agtttgcgct ttcattcaga aaacatctgc
     4141 tgaaaaggca gccgcagaga tcgataggcg tctaagcacc gatttcgaga ccagaaaaat
     4201 tgctcgtgaa gagggcaacc gctttgtgga cgcccagatc ctcacctatc aacaggagcg
     4261 tcttccagaa gcagtgcgca tcaaggtggg cgcggctcca gctacacttt atgctgtgta
     4321 ctcggagttc gctagaagca tacctggctt ccagcagatg agcgatcgcg atattgccct
     4381 gtttgtgccc aagccgacgg atttgtctca gccaaatgtg tttgctaatg atgatagcat
     4441 ggtatacggg gaactggcta gcaaaatgga ggccttcatg aacacggcaa tcggagtgcc
     4501 gacactccag atccaggcta gcaagatgca catgctactc aacgccctga tagcgacgcg
     4561 tcgactgcgt gaccaagagt ctgcctttaa tctgctgacc cgcgcggtcg agggtttgac
     4621 cgaaggattg gtcaatatgc atgaaaacat ggagcaaatg aagatgtacc aaaacatcca
     4681 tctccgtatc cttggcttgc tgaacaacag tttcggtgct ccaaacacag agcgagcggt
     4741 aacaaagtgt ttcttcgata tccgagagga ggtgcgatat aacgtggagg cagctcgcgc
     4801 tttgattacg tcgcactttg tcaatctgaa tcagtttgac ggaatgctac gcgactgcat
     4861 ggacaacgga aacaattatg tggctatatc gttcggtatc gcactgctgg agcgcctcat
     4921 catggacgat cgagtgatta atattgtttc ggacaacgaa ttcatggcaa ctgttgagct
     4981 gctaggtagg ctgacccaac accgtcaccg ctaccccgag tgtatcgtca acgccatcga
     5041 caccctgtgg tccggcaatt tcaatagctc ctccgattat tcccccttta atggaaacga
     5101 ccgctatctg agcggcgcta gccactatat tcatagcgga atgcaccatg tccgctcgtg
     5161 tgacaccgac gacccccccg gactccagga aaaaaccgaa ttcttgttga aagactgggt
     5221 ggccttgtat acccagcaaa atcaacaatc gacccgtgac gcccgtaatt ttggcgcctt
     5281 cgtgcaaaaa atgaatacct acggcatttt gaagaccgat gatctgatta cgcgcttctt
     5341 tcgtcaagct acccatatct gtaccgacgt cgtctatcgt atgtttgctg aacccagcct
     5401 tcccattaat caagctaaga ataaaatttt tcaatggatt gacgcctttg tgcacttgat
     5461 cgccatgctg gtccgccatt cgggagaggc cggcaacccc acgacgaaaa ttaatttgct
     5521 caataaagtc ttgggaatcg tcttgggcac cttgatcaaa gatcacgaaa tgcgcggagt
     5581 gtcctttcaa caagtgggat atcaccgttt ttttatgatg ttgtttatgg aattgtgtac
     5641 cgctgacgtc atcctggaaa gcttgatgca tagcatcgtc agcgcttttg cttacacata
     5701 tcacttgttg aatcccagcg tggctccggg attctgcttt gcttggttgg agctcatttc
     5761 gcatcgcgtc ttcctgggcc gcatcctggt ccaaattccc gggcaaaagg gctggccact
     5821 ttacgctcaa ttgctgcagg acctttttaa atatcttgcg cccttcttac gcaacactga
     5881 gctcggcaaa ccagtccagc ttttgtacaa ggggacgcta cgtgttctgc tcgttctgct
     5941 tcacgacttc ccggagttct tgtgcgacta tcattttggg ttctgtgaca ccatcccgcc
     6001 caattgcgtc cagatgcgta acatcatcct gtcggcgttt ccgcgcaaca tgcgcttgcc
     6061 cgatcccttc acgcccaacc taaaagtgga catgctgtcg gacagtagta atgcgcctaa
     6121 ggtgctcagt agctacatta tgaacatcca gcccgcgaac tttaagaagg atttggactc
     6181 gtacctcaag gcccgggcgc cagttacttt tctatcggaa ttacgtggtc acttgcaggt
     6241 gacaagcgag ccaggaactc gttacaacat ggcgctgatg aatgctctgg tcatgtatgt
     6301 gggaactcag gcgattgctt tgatcagaaa caaaaacttt gtgcccaaca cctcgaacat
     6361 agcacacagc gcccacatgg atatcttcca gaatcttgct gttgacttgg atacggaggg
     6421 tcgctatctg ttcttgaatg cgattgcgaa ccagttgcgc tatcccaaca gccacacgca
     6481 ctacttcagc tgtgcggtgc tgcatctgtt tgccgaggcc aactcggagg caattcagga
     6541 gcagatcact cgcgtacttc tggaacgatt gatcgtgaac cgcccgcatc cttggggact
     6601 gttgatcact tttattgagc tgatcaaaaa tcccatctac aagttctggg accatgactt
     6661 tgtgcactgt gcgccagaga tcaccaagct ctttgaatcg gtggcccgtt cctgtctggc
     6721 caagtcgaac gtcacccagc agttgaacat gcccgtcgtc gatggcgagg gccaggaggt
     6781 agccaccatc aactgatcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     6841 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     6901 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     6961 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     7021 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     7081 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     7141 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     7201 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     7261 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     7321 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     7381 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     7441 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     7501 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     7561 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     7621 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag
     7681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     7921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     8161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8341 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8401 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8761 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     8821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9061 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     9121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     9481 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
