LOCUS       sequence-289907-        9847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsSmg6-sh(10)RNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9847)
  AUTHORS   Jonas S, Weichenrieder O, Izaurralde E
  TITLE     An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 
            heterodimer is required for efficient nonsense-mediated mRNA decay.
  JOURNAL   Genes Dev. 2013 Jan 15;27(2):211-25. doi: 10.1101/gad.206672.112.
  PUBMED    23348841
REFERENCE   2  (bases 1 to 9847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.206672"; journalName: "Genes Dev"; date: "2013-01-15- 
            15"; volume: "27"; issue: "2"; pages: "211-25"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsSmg6-sh(t10)RNAres_S
FEATURES             Location/Qualifiers
     source          1..9847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1098..1107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1104..1169
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1194..1220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    5551..5672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5562..5581)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5616..5635
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5858..6313
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5945..5964)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6155..6176
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6430..6787
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6430..6621
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6453..6473)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6638..6773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6838..7632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6892..6911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7502..7521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7696..7744
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7785..7804)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7904..7926
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7964..7982)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8050..8154
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8155..8223,8224..9015)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8373..8392)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9186..9774
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc aattgcggcc accatggacg cacaaacacg acgacgtgag cgtcgcgctg
     1141 agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc tccacatatg gcttacccat
     1201 acgatgttcc agattacgct cctccactcg agaattcacg cgtgatggcg gaagggctgg
     1261 agcgtgtgcg gatctccgcg tcggagctgc gcgggatcct ggctactctg gccccgcagg
     1321 ccgggagcag agaaaacatg aaggaattaa aggaggccag gccgcgcaaa gataacaggc
     1381 gtccagatct ggaaatctat aagcctggcc tttctcggct aaggaacaag cccaaaatca
     1441 aggaaccccc tgggagtgag gaattcaaag atgaaattgt taatgaccga gattgctctg
     1501 ctgttgaaaa tggtacacag cccgttaaag atgtctgcaa ggaactgaac aaccaagagc
     1561 agaatggtcc tatagaccca gaaaataatc ggggacaaga atcctttcct aggactgctg
     1621 gacaagagga tcgtagtcta aaaattatca aaagaacaaa gaaacccgac ctgcagatct
     1681 atcagcctgg acgacgtttg cagactgtta gcaaagaatc cgccagtcgg gtggaggagg
     1741 aagaagtcct caaccaggta gaacaactga gagtagagga agatgagtgt aggggaaatg
     1801 ttgcgaagga ggaagttgcg aataaaccag acagggccga gatagaaaag agcccaggtg
     1861 gtgggagagt aggggctgca aaaggagaaa aaggaaagag gatgggaaaa ggggaggggg
     1921 tgagggaaac ccacgacgac ccggcccgcg ggaggccggg ctccgcaaag cgctactccc
     1981 gctcagacaa acgaaggaat cgctaccgca cgcgcagcac cagctcagct ggcagcaaca
     2041 acagcgctga gggagctggc ctgacggata atggatgtcg ccgccgccga caggatagga
     2101 ccaaggagag gccaccactg aagaagcaag tgtctgtgtc ctcaaccgat tccttagacg
     2161 aggacagaat tgatgagcct gatggattag gacccaggag aagttcagaa aggaagagac
     2221 atttagaaag aaactggtct ggccgtgggg agggtgagca gaaaaccagt gctaaagaat
     2281 atcgaggcac tcttcgtgtc actttcgatg cagaagccat gaacaaagag tctcccatgg
     2341 tgaggtcagc cagggatgat atggatagag gaaagcctga caaaggcttg agcagtgggg
     2401 gcaaaggctc tgagaagcag gagtccaaaa acccgaaaca agaacttcgg ggtcgtggtc
     2461 gtggcattct gattttgcct gcccatacca ccctatctgt caattcagca ggttctccag
     2521 agtccgcgcc tttgggacct cggcttttgt ttggatctgg tagtaaggga tctcggagtt
     2581 ggggccgtgg aggcaccaca cgccgattgt gggacccaaa caatcctgat cagaaacctg
     2641 ctctaaagac tcagacgccc cagctacatt tcttggacac tgatgatgaa gtcagcccta
     2701 catcttgggg tgactcacgc caggctcagg catcttacta taagtttcaa aactctgaca
     2761 acccctatta ttacccccgg acaccaggcc ctgcctccca gtatccctat acgggctata
     2821 accctctaca gtacccagtg ggccctacga atggtgtgta cccagggcct tactacccag
     2881 gctacccgac tccgtcagga cagtatgtgt gtagccctct acctaccagc accatgagtc
     2941 ccgaggaggt agagcagcac atgaggaacc tgcagcaaca ggagctgcac aggcttctcc
     3001 gggtggctga caaccaggaa ctgcagctca gcaacctgct ctccagggac cgcatcagtc
     3061 cggagggcct ggagaagatg gcgcaactca gagctgaact gctgcagcta tatgagcgct
     3121 gtattctatt agatattgag ttctctgata atcagaatgt ggatcagatc ctgtggaaga
     3181 atgctttcta tcaggtgatt gagaagttca ggcaacttgt caaggatccg aatgttgaga
     3241 acccagaaca gattcggaac agacttttgg agctcttgga tgagggtagt gacttctttg
     3301 atagtttgct tcagaaactc caagtgacct ataaattcaa actggaagac tacatggatg
     3361 gtcttgccat tcgcagcaag ccattacgca agacagtaaa atatgccttg atcagtgccc
     3421 agcgatgcat gatatgccaa ggagatattg ctaggtaccg ggagcaagcc agtgatacag
     3481 cgaattatgg gaaagcacgc agttggtacc tgaaggccca gcacattgct cccaagaatg
     3541 ggcgccccta taaccagttg gctttgctgg cagtgtatac gaggaggaag cttgacgctg
     3601 tctattacta tatgcgcagt ttagctgcca gcaaccctat cctgactgcc aaggagagtc
     3661 tcatgagctt gtttgaagag accaagcgga aggcagaaca gatggaaaag aagcaacatg
     3721 aggaatttga cctgagccct gaccagtggc ggaaaggaaa gaagtctact ttccggcatg
     3781 ttggagatga caccactcgc ctggagatct ggattcatcc atcccatcca cggtcttccc
     3841 agggcactga gtctgggaag gattctgagc aagagaatgg gctgggcagc ctgagtccca
     3901 gtgatctgaa caaaaggttc atcctcagtt ttctccatgc ccatgggaag ctgtttaccc
     3961 ggattgggat ggagacattc cctgcagtgg ctgagaaggt cctcaaggag ttccaggtgt
     4021 tactgcagca cagcccctct cccattggaa gtacccgcat gctgcagctt atgaccatca
     4081 atatgtttgc agtacacaac tcccagctga aagactgctt ctcggaggag tgccgctctg
     4141 tgatccagga acaagccgca gctctgggct tggccatgtt ttctctactg gtccgccgct
     4201 gcacctgctt acttaaggag tccgccaaag ctcagctgtc ctctcctgag gaccaggatg
     4261 accaagacga catcaaggtg tcttcctttg tcccggacct gaaggagctg ctccccagtg
     4321 tcaaagtctg gtcagattgg atgctcggct acccggacac ctggaatcct cctcccacat
     4381 ccctggatct gccctcgcat gttgctgtgg atgtatggtc gacgctggct gatttctgta
     4441 acatactgac tgcagtgaat cagtctgagg tgccactgta caaggacccg gatgatgacc
     4501 tcacccttct tatcctggaa gaggatcggc ttctctcggg ctttgtcccc ttgctggctg
     4561 cccctcagga cccctgctac gtggagaaaa cctcggataa ggttattgca gctgactgca
     4621 aaagggtcac agtgctgaag tattttctgg aagccctttg tggacaagaa gagcctctgc
     4681 tggcattcaa gggtggaaag tatgtgtcag tggcacccgt cccagacacc atgggaaagg
     4741 aaatgggaag ccaagaggga acacgactgg aagatgagga ggaggatgtg gtgattgaag
     4801 actttgagga agattcagag gctgaaggca gcggaggcga ggatgacatc agggagcttc
     4861 gggccaagaa gctggctctg gccaggaaga tagctgagca gcagcgtcgc caggaaaaga
     4921 tccaggctgt cctggaggac cacagtcaga tgaggcagat ggagctcgaa atcagacctt
     4981 tgttcctcgt accagacacc aacggcttca ttgaccacct ggccagtctg gcgcggctgc
     5041 tggagagcag gaagtacatc ctggtggtgc ccctcatcgt gatcaatgag ctggacggcc
     5101 tggccaaggg gcaggagaca gaccaccggg ctgggggcta cgcccgtgtg gtacaagaga
     5161 aggcccgcaa gtccatcgag ttcctcgagc agcgattcga gagtcgggac tcttgcctgc
     5221 gagccctgac cagccgtggc aatgaactcg aatccatcgc cttccgcagt gaggacatca
     5281 ctggccagct gggtaacaac gatgatctca tcctgtcctg ctgcctccac tactgcaaag
     5341 acaaggctaa ggacttcatg cccgccagca aagaggagcc aatccggcta ctgcgggagg
     5401 tggtgctgtt gacggatgac cggaacctgc gtgtgaaggc gctcacaagg aatgttcctg
     5461 tacgggacat cccagccttc ctcacgtggg cccaggtggg ctgagcggcc gcttcccttt
     5521 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
     5581 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5641 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5701 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     5761 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     5821 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     5881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6061 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6181 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6301 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     6361 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     6421 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     6481 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6541 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6601 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6781 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg
     6841 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     6901 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     6961 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     7021 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     7081 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     7141 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     7201 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     7261 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     7321 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     7381 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     7441 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     7501 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     7561 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     7621 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     7681 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     7741 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat
     7801 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7861 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7921 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7981 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8041 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8101 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8161 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8221 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8281 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8341 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8401 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8461 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8521 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8581 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8641 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8701 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8761 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8821 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8881 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8941 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9001 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9061 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9121 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9181 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9241 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9301 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9361 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9421 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9481 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9541 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9601 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9661 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9721 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9781 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catggctcga
     9841 cagatct
//
