LOCUS       sequence-289931-       10395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-del1844-2133-V1251R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Boland A, Chen Y, Raisch T, Jonas S, Kuzuoglu-Ozturk D, Wohlbold L, 
            Weichenrieder O, Izaurralde E
  TITLE     Structure and assembly of the NOT module of the human CCR4-NOT 
            complex.
  JOURNAL   Nat Struct Mol Biol. 2013 Nov;20(11):1289-97. doi: 
            10.1038/nsmb.2681. Epub 2013 Oct 13.
  PUBMED    24121232
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2681";
            journalName: "Nat Struct Mol Biol"; date: "2013-11"; volume: "20"; 
            issue: "11"; pages: "1289-97"
COMMENT     Sequence Label: pLNHA-C1-HsNot1iso1-del1844-2133-V1251R_S
FEATURES             Location/Qualifiers
     source          1..10395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    7183..7304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7220..7239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7274..7293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7311..7766)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7448..7469)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7660..7679
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7793..7897
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7899..8256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7899..8090
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7922..7942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8107..8242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8291..9085
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8291..9085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8345..8364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8955..8974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9273..9292)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9317..9364
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9693..10281
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10182..10201
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctatga atcttgactc gctctcgctg gccttgtctc aaatcagcta
      841 cctggtggac aatttaacca agaaaaatta ccgagccagc cagcaggaaa tacagcatat
      901 tgtgaatcgg cacggtcctg aggcagacag gcatttatta cgctgcctat tttcgcatgt
      961 ggatttcagt ggcgatggta aaagcagtgg caaagatttc catcagactc agtttctgat
     1021 tcaggagtgt gcgttgctga ttacaaagcc aaattttatc tcgacgctgt cctatgccat
     1081 tgataatcca ttgcactatc agaagagttt aaagcctgca ccccacttat ttgcccagct
     1141 gagtaaagtg ctcaaattaa gcaaagtaca agaggtaatt tttggccttg ccctgttgaa
     1201 ttcttccagc tcagatctta gaggtttcgc tgcccagttt atcaaacaga agcttccaga
     1261 tcttctgcgt tcttacattg acgcagacgt cagtggaaat caagaaggtg gcttccaaga
     1321 tatagcaata gaggtcctac acctcctcct ctcccatctc ctctttgggc agaagggagc
     1381 ctttggagtt ggacaagaac agatagacgc ttttcttaag acgctgcgca gagattttcc
     1441 ccaagaacgc tgtcccgtgg tgctcgcacc acttttatac cctgaaaaac gggacattct
     1501 aatggacagg atcctgcctg attccggagg ggtagctaaa accatgatgg agagctcttt
     1561 ggctgatttc atgcaagaag taggctatgg cttttgtgca agtattgaag aatgtcgcaa
     1621 tataatcgtg cagtttggtg ttcgggaggt cacagctgcc caggttgcaa gggttttggg
     1681 aatgatggct cgaactcatt caggattaac agatggcatt ccattacaga gtatttctgc
     1741 tccgggcagt gggatctgga gtgatgggaa agataaaagt gatggagcac aggcacacac
     1801 atggaatgta gaagtcttga ttgacgttct taaagaactg aatccaagtt tgaatttcaa
     1861 ggaagtaact tatgaactgg accatcctgg atttcaaatt cgtgacagta aaggacttca
     1921 taatgtggtt tatggcattc agaggggttt gggtatggaa gtgttcccag tagacctcat
     1981 atatagacct tggaaacatg ctgaaggcca gctctccttc attcaacatt cccttataaa
     2041 tccagagatc ttctgttttg ctgactatcc ctgtcatact gttgccactg atattctgaa
     2101 agcaccacca gaggatgaca atcgagaaat tgccacatgg aagagcttgg atttgattga
     2161 atctctgctg aggcttgcag aggttgggca gtatgagcaa gtcaaacagc tcttcagctt
     2221 ccctatcaaa cactgtccag acatgctggt attggcctta ctacaaatta acacctcttg
     2281 gcataccttg cgccatgaac ttatctccac tctgatgcca attttccttg gaaaccatcc
     2341 taactcagct attattttgc actatgcatg gcatgggcag ggacagtctc cctcaattcg
     2401 ccaacttatc atgcatgcaa tggcagaatg gtacatgaga ggggagcagt atgatcaggc
     2461 caaattgtct cgaatacttg atgtggccca ggacttgaag gccttgtcaa tgctgctaaa
     2521 tggtactcca tttgcctttg ttattgacct tgctgcactt gcttcacgtc gtgaatacct
     2581 caaacttgat aagtggctca cagataaaat tcgagagcat ggggagcctt ttatccaggc
     2641 gtgtatgact tttttaaaga gacggtgtcc ttctattttg ggcggacttg ccccagaaaa
     2701 agaccagccc aaaagtgctc aacttcctcc agaaactttg gcgacaatgt tggcctgtct
     2761 gcaagcttgt gcagggagtg tttctcagga gctatcagaa actatcctca ccatggtagc
     2821 caattgcagt aatgttatga ataaggccag acaaccacca cctggagtta tgccaaaagg
     2881 acgtcctcct agtgctagca gcttagatgc catttctcct gttcagattg accctcttgc
     2941 tggaatgaca tctcttagta taggtggttc agctgcccct cacacccaga gtatgcaggg
     3001 ttttcctcca aatttgggtt ctgcattcag tacccctcag tcaccagcaa aagcatttcc
     3061 acccctttca acccccaatc agaccactgc attcagtggt attggaggac tttcatcaca
     3121 gcttccagta ggtggtcttg gcacaggcag cctgactggt ataggaactg gtgctcttgg
     3181 actccctgca gtgaataacg acccttttgt acagaggaaa ctgggcacct ctggactgaa
     3241 tcagcctaca ttccagcaga cggacttgtc tcaggtgtgg ccagaggcaa accagcactt
     3301 tagtaaagag atagatgatg aagcaaacag ctatttccag cgaatatata atcatccacc
     3361 acatccaacc atgtctgttg atgaggtatt agaaatgctg cagagattta aagactctac
     3421 tataaagagg gaacgagaag tatttaactg tatgctaagg aacttgtttg aagaatatcg
     3481 tttttttccc cagtatcctg ataaagagtt acatataaca gcctgcctat ttggtggtat
     3541 aattgagaaa ggactggtca cttacatggc actaggtctg gctctacgat atgttcttga
     3601 agccttacgc aagccttttg gatccaaaat gtattatttc gggattgctg cactagatag
     3661 atttaaaaac agattgaagg actatcccca gtattgtcag catttggctt ctatcagtca
     3721 ctttatgcaa tttccacatc atttacagga gtatattgag tatggacagc agtctagaga
     3781 tcctcctgtg aaaatgcaag gctctatcac aacccctgga agtattgcac tggctcaggc
     3841 ccaggctcag gcccaggttc cagcaaaagc tcctcttgct ggtcaagtta gcactatggt
     3901 aaccacctca acaactacca ctgttgctaa aacggttacg gtcaccaggc caactggagt
     3961 cagctttaag aaagatgtgc caccttctat taatactaca aatatagata cgttgcttgt
     4021 ggccacagat caaactgaga gaattgtgga gcccccagaa aatatccagg agaaaattgc
     4081 ttttattttc aataatctct cacagtcaaa tatgacacaa aaggttgaag agctaaagga
     4141 aacggtgaaa gaagaattta tgccttgggt ttcacagtat ctggttatga agagagtcag
     4201 tattgagcca aactttcata gcctgtattc aaacttcctt gacacgctga agaatcctga
     4261 atttaacaag atggttctga atgagaccta cagaaacatt aaagtgctcc tgacctctga
     4321 taaagctgca gccaatttct cagatcgttc tttgctgaag aacttgggac attggctagg
     4381 aatgatcaca ttagctaaaa acaaacccat cttacacact gacttggatg tgaaatcatt
     4441 gctgctagag gcttatgtta aaggacaaca agaattgctc tatgtagtgc cctttgttgc
     4501 caaagtctta gaatctagca ttcgtagtgt gcgttttagg ccaccaaacc cttggacaat
     4561 ggcaattatg aatgtattag ctgagctaca tcaggagcat gacttaaagt taaacttgaa
     4621 gtttgaaatc gaggttctct gcaagaacct tgcattagac atcaatgagc taaaacctgg
     4681 aaacctccta aaggataaag atcgcctgaa gaatttagat gagcaactct ctgctccaaa
     4741 gaaagatgtc aagcagccag aagaactccc tcccatcaca accacaacaa cttctactac
     4801 accagctacc aacaccactt gtacagccac ggttccacca cagccacagt acagctacca
     4861 cgacatcaat gtctattccc ttgcgggctt ggcaccacac attactctaa atccaacaat
     4921 tcccttgttt caggcccatc cacagttgaa gcagtgtgtg cgtcaggcaa ttgaacgggc
     4981 tgtccaggag ctggtccatc ctgtggtgga tcgatcaatt aagattgcca tgactacttg
     5041 tgagcaaata gtcaggaagg attttgccct ggattcggag gaatctcgaa tgcgaatagc
     5101 agctcatcac atgatgcgta acttgacagc tggaatggct atgattacat gcagggaacc
     5161 tttgctcatg agcatatcta ccaacttaaa aaacagtttt gcctcagccc ttcgtactgc
     5221 ttccccacaa caaagagaaa tgatggatca ggcagctgct caattagctc aggacaattg
     5281 tgagttggct tgctgtttta ttcagaagac tgcagtagaa aaagcaggcc ctgagatgga
     5341 caagagatta gcaactgaat ttgagctgag aaaacatgct aggcaagaag gacgcagata
     5401 ctgtgatcct gttgttttaa catatcaagc tgaacggatg ccagagcaaa tcaggctgaa
     5461 agttggtggt gtggacccaa agcagttggc tgtttacgaa gagtttgcac gcaatgttcc
     5521 tggcttctta cctacaaatg acttaagtca gcccacggga tttttagccc agcccatgaa
     5581 gcaagcttgg gcaacagatg atgtagctca gatttatgat aagtgtatta cagaactgga
     5641 gcaacatcta catgccatcc caccaacttt ggccatgaac cctcaagctc aggctcttcg
     5701 aagtctcttg gaggttgtag ttttatctcg aaactctcgg gatgccatag ctgctcttgg
     5761 attgctccaa aaggctgtag agggcttact agatgccaca agtggtgctg atgctgacct
     5821 tctgctgcgc tacagggaat gccacctctt ggtcctaaaa gctctgcagg atggccgggc
     5881 atatgggtct ccatggtgca acaaacagat cacaaggtgc ctaattgaat gtcgagatga
     5941 atataaatat aatgtggagg ctgtggagct gctaattcgc aatcatttgg ttaatatgca
     6001 gcagtatgat cttcacctag cgcagtcaat ggagaatggc ttaaactaca tggctgtggc
     6061 atttgctatg cagttagtaa aaatcctgct ggtggatgaa aggagtgttg ctcatgttac
     6121 tgaggcagat ctgttccaca ccattgaaac cctcatgagg attaatgctc attccagagg
     6181 caatgctcca gaaggattgc cccagctgat ggaagtagtg cgatccaact atgaagcaat
     6241 gattgatcgt gctcatggag gcccaaactt tatgatgcat tctgggatct ctcaagcctc
     6301 agagtatgat agtgcctttc caagaaacat gaggctcccc gacccattca ctcctaatct
     6361 aaaggtggac atgttgagtg aaattaacat tgctccccgg attctcacca atttcactgg
     6421 agtaatgcca cctcagttca aaaaggattt ggattcctat cttaaaactc gatcaccagt
     6481 cactttcctg tctgatctgc gcagcaacct acaggtatcc aatgaacctg ggaatcgcta
     6541 caacctccag ctcatcaatg cactggtgct ctatgtcggg actcaggcca ttgcgcacat
     6601 ccacaacaag ggcagcacac cttcaatgag caccatcact cactcagcac acatggatat
     6661 cttccagaat ttggctgtgg acttggacac tgagggtcgc tatctctttt tgaatgcaat
     6721 tgcaaatcag ctccggtacc caaatagcca cactcactac ttcagttgca ccatgctgta
     6781 cctttttgca gaggccaata cggaagccat ccaagaacag atcacaagag ttctcttgga
     6841 acggttgatt gtaaataggc cacatccttg gggtcttctt attaccttca ttgagctgat
     6901 taaaaaccca gcgtttaagt tctggaacca tgaatttgta cactgtgccc cagaaatcga
     6961 aaagttattc cagtcggtcg cacagtgctg catgggacag aagcaggccc agcaagtaat
     7021 ggaagggaca ggtgccagtt agccgcgggc ccgggatcca ccggatctag ataactgatc
     7081 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     7141 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     7201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     7321 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7381 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     7441 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     7501 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     7561 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     7621 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     7681 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     7741 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     7801 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7861 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     7921 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7981 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     8041 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8101 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8161 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8221 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     8281 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     8341 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     8401 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     8461 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     8521 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     8581 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     8641 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     8701 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     8761 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     8821 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     8881 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8941 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     9001 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     9061 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     9121 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     9181 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     9241 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     9301 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     9361 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     9421 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     9481 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     9541 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9601 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9661 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9721 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9781 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     9841 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9901 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9961 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10021 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10081 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10141 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10201 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10261 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10321 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10381 attaccgcca tgcat
//
