LOCUS       sequence-289978-        8079 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1_4-1317.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pLNHA-C1-HsNot1iso1_4-1317_S
FEATURES             Location/Qualifiers
     source          1..8079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4867..4988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4904..4923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4958..4977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4995..5450)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5132..5153)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5344..5363
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5477..5581
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5583..5940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5583..5774
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5606..5626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5791..5926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5975..6769
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5975..6769
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6029..6048)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6639..6658
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6957..6976)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7001..7048
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7377..7965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7866..7885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctgact cgctctcgct ggccttgtct caaatcagct acctggtgga
      841 caatttaacc aagaaaaatt accgagccag ccagcaggaa atacagcata ttgtgaatcg
      901 gcacggtcct gaggcagaca ggcatttatt acgctgccta ttttcgcatg tggatttcag
      961 tggcgatggt aaaagcagtg gcaaagattt ccatcagact cagtttctga ttcaggagtg
     1021 tgcgttgctg attacaaagc caaattttat ctcgacgctg tcctatgcca ttgataatcc
     1081 attgcactat cagaagagtt taaagcctgc accccactta tttgcccagc tgagtaaagt
     1141 gctcaaatta agcaaagtac aagaggtaat ttttggcctt gccctgttga attcttccag
     1201 ctcagatctt agaggtttcg ctgcccagtt tatcaaacag aagcttccag atcttctgcg
     1261 ttcttacatt gacgcagacg tcagtggaaa tcaagaaggt ggcttccaag atatagcaat
     1321 agaggtccta cacctcctcc tctcccatct cctctttggg cagaagggag cctttggagt
     1381 tggacaagaa cagatagacg cttttcttaa gacgctgcgc agagattttc cccaagaacg
     1441 ctgtcccgtg gtgctcgcac cacttttata ccctgaaaaa cgggacattc taatggacag
     1501 gatcctgcct gattccggag gggtagctaa aaccatgatg gagagctctt tggctgattt
     1561 catgcaagaa gtaggctatg gcttttgtgc aagtattgaa gaatgtcgca atataatcgt
     1621 gcagtttggt gttcgggagg tcacagctgc ccaggttgca agggttttgg gaatgatggc
     1681 tcgaactcat tcaggattaa cagatggcat tccattacag agtatttctg ctccgggcag
     1741 tgggatctgg agtgatggga aagataaaag tgatggagca caggcacaca catggaatgt
     1801 agaagtcttg attgacgttc ttaaagaact gaatccaagt ttgaatttca aggaagtaac
     1861 ttatgaactg gaccatcctg gatttcaaat tcgtgacagt aaaggacttc ataatgtggt
     1921 ttatggcatt cagaggggtt tgggtatgga agtgttccca gtagacctca tatatagacc
     1981 ttggaaacat gctgaaggcc agctctcctt cattcaacat tcccttataa atccagagat
     2041 cttctgtttt gctgactatc cctgtcatac tgttgccact gatattctga aagcaccacc
     2101 agaggatgac aatcgagaaa ttgccacatg gaagagcttg gatttgattg aatctctgct
     2161 gaggcttgca gaggttgggc agtatgagca agtcaaacag ctcttcagct tccctatcaa
     2221 acactgtcca gacatgctgg tattggcctt actacaaatt aacacctctt ggcatacctt
     2281 gcgccatgaa cttatctcca ctctgatgcc aattttcctt ggaaaccatc ctaactcagc
     2341 tattattttg cactatgcat ggcatgggca gggacagtct ccctcaattc gccaacttat
     2401 catgcatgca atggcagaat ggtacatgag aggggagcag tatgatcagg ccaaattgtc
     2461 tcgaatactt gatgtggccc aggacttgaa ggccttgtca atgctgctaa atggtactcc
     2521 atttgccttt gttattgacc ttgctgcact tgcttcacgt cgtgaatacc tcaaacttga
     2581 taagtggctc acagataaaa ttcgagagca tggggagcct tttatccagg cgtgtatgac
     2641 ttttttaaag agacggtgtc cttctatttt gggcggactt gccccagaaa aagaccagcc
     2701 caaaagtgct caacttcctc cagaaacttt ggcgacaatg ttggcctgtc tgcaagcttg
     2761 tgcagggagt gtttctcagg agctatcaga aactatcctc accatggtag ccaattgcag
     2821 taatgttatg aataaggcca gacaaccacc acctggagtt atgccaaaag gacgtcctcc
     2881 tagtgctagc agcttagatg ccatttctcc tgttcagatt gaccctcttg ctggaatgac
     2941 atctcttagt ataggtggtt cagctgcccc tcacacccag agtatgcagg gttttcctcc
     3001 aaatttgggt tctgcattca gtacccctca gtcaccagca aaagcatttc cacccctttc
     3061 aacccccaat cagaccactg cattcagtgg tattggagga ctttcatcac agcttccagt
     3121 aggtggtctt ggcacaggca gcctgactgg tataggaact ggtgctcttg gactccctgc
     3181 agtgaataac gacccttttg tacagaggaa actgggcacc tctggactga atcagcctac
     3241 attccagcag acggacttgt ctcaggtgtg gccagaggca aaccagcact ttagtaaaga
     3301 gatagatgat gaagcaaaca gctatttcca gcgaatatat aatcatccac cacatccaac
     3361 catgtctgtt gatgaggtat tagaaatgct gcagagattt aaagactcta ctataaagag
     3421 ggaacgagaa gtatttaact gtatgctaag gaacttgttt gaagaatatc gtttttttcc
     3481 ccagtatcct gataaagagt tacatataac agcctgccta tttggtggta taattgagaa
     3541 aggactggtc acttacatgg cactaggtct ggctctacga tatgttcttg aagccttacg
     3601 caagcctttt ggatccaaaa tgtattattt cgggattgct gcactagata gatttaaaaa
     3661 cagattgaag gactatcccc agtattgtca gcatttggct tctatcagtc actttatgca
     3721 atttccacat catttacagg agtatattga gtatggacag cagtctagag atcctcctgt
     3781 gaaaatgcaa ggctctatca caacccctgg aagtattgca ctggctcagg cccaggctca
     3841 ggcccaggtt ccagcaaaag ctcctcttgc tggtcaagtt agcactatgg taaccacctc
     3901 aacaactacc actgttgcta aaacggttac ggtcaccagg ccaactggag tcagctttaa
     3961 gaaagatgtg ccaccttcta ttaatactac aaatatagat acgttgcttg tggccacaga
     4021 tcaaactgag agaattgtgg agcccccaga aaatatccag gagaaaattg cttttatttt
     4081 caataatctc tcacagtcaa atatgacaca aaaggttgaa gagctaaagg aaacggtgaa
     4141 agaagaattt atgccttggg tttcacagta tctggttatg aagagagtca gtattgagcc
     4201 aaactttcat agcctgtatt caaacttcct tgacacgctg aagaatcctg aatttaacaa
     4261 gatggttctg aatgagacct acagaaacat taaagtgctc ctgacctctg ataaagctgc
     4321 agccaatttc tcagatcgtt ctttgctgaa gaacttggga cattggctag gaatgatcac
     4381 attagctaaa aacaaaccca tcttacacac tgacttggat gtgaaatcat tgctgctaga
     4441 ggcttatgtt aaaggacaac aagaattgct ctatgtagtg ccctttgttg ccaaagtctt
     4501 agaatctagc attcgtagtg tggtttttag gccaccaaac ccttggacaa tggcaattat
     4561 gaatgtatta gctgagctac atcaggagca tgacttaaag ttaaacttga agtttgaaat
     4621 cgaggttctc tgcaagaacc ttgcattaga catcaatgag ctaaaacctg gaaacctcct
     4681 aaaggataaa gatcgcctga agaatttaga tgagcaactc tcttaaccgc gggcccggga
     4741 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4801 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4861 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4921 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4981 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     5041 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     5101 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     5161 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     5221 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     5281 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     5341 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     5401 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac
     5461 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5521 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5581 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5701 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5761 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5821 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     5881 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5941 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6001 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6061 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6121 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
     6181 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6241 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6301 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6361 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6421 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6481 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6541 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6601 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6661 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6721 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6781 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6841 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6901 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     6961 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     7021 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     7081 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     7141 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     7201 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     7261 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7501 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8041 tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
