LOCUS       sequence-289985-        9478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1_4-1580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1_4-1580_S
FEATURES             Location/Qualifiers
     source          1..9478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6266..6387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6303..6322)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6357..6376
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6394..6849)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6531..6552)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6743..6762
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6876..6980
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6982..7339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6982..7173
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7005..7025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7190..7325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7374..8168
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7374..8168
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7428..7447)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8038..8057
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8356..8375)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8400..8447
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8776..9364
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9265..9284
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctgactcgct ctcgctggcc ttgtctcaaa tcagctacct
     1441 ggtggacaat ttaaccaaga aaaattaccg agccagccag caggaaatac agcatattgt
     1501 gaatcggcac ggtcctgagg cagacaggca tttattacgc tgcctatttt cgcatgtgga
     1561 tttcagtggc gatggtaaaa gcagtggcaa agatttccat cagactcagt ttctgattca
     1621 ggagtgtgcg ttgctgatta caaagccaaa ttttatctcg acgctgtcct atgccattga
     1681 taatccattg cactatcaga agagtttaaa gcctgcaccc cacttatttg cccagctgag
     1741 taaagtgctc aaattaagca aagtacaaga ggtaattttt ggccttgccc tgttgaattc
     1801 ttccagctca gatcttagag gtttcgctgc ccagtttatc aaacagaagc ttccagatct
     1861 tctgcgttct tacattgacg cagacgtcag tggaaatcaa gaaggtggct tccaagatat
     1921 agcaatagag gtcctacacc tcctcctctc ccatctcctc tttgggcaga agggagcctt
     1981 tggagttgga caagaacaga tagacgcttt tcttaagacg ctgcgcagag attttcccca
     2041 agaacgctgt cccgtggtgc tcgcaccact tttataccct gaaaaacggg acattctaat
     2101 ggacaggatc ctgcctgatt ccggaggggt agctaaaacc atgatggaga gctctttggc
     2161 tgatttcatg caagaagtag gctatggctt ttgtgcaagt attgaagaat gtcgcaatat
     2221 aatcgtgcag tttggtgttc gggaggtcac agctgcccag gttgcaaggg ttttgggaat
     2281 gatggctcga actcattcag gattaacaga tggcattcca ttacagagta tttctgctcc
     2341 gggcagtggg atctggagtg atgggaaaga taaaagtgat ggagcacagg cacacacatg
     2401 gaatgtagaa gtcttgattg acgttcttaa agaactgaat ccaagtttga atttcaagga
     2461 agtaacttat gaactggacc atcctggatt tcaaattcgt gacagtaaag gacttcataa
     2521 tgtggtttat ggcattcaga ggggtttggg tatggaagtg ttcccagtag acctcatata
     2581 tagaccttgg aaacatgctg aaggccagct ctccttcatt caacattccc ttataaatcc
     2641 agagatcttc tgttttgctg actatccctg tcatactgtt gccactgata ttctgaaagc
     2701 accaccagag gatgacaatc gagaaattgc cacatggaag agcttggatt tgattgaatc
     2761 tctgctgagg cttgcagagg ttgggcagta tgagcaagtc aaacagctct tcagcttccc
     2821 tatcaaacac tgtccagaca tgctggtatt ggccttacta caaattaaca cctcttggca
     2881 taccttgcgc catgaactta tctccactct gatgccaatt ttccttggaa accatcctaa
     2941 ctcagctatt attttgcact atgcatggca tgggcaggga cagtctccct caattcgcca
     3001 acttatcatg catgcaatgg cagaatggta catgagaggg gagcagtatg atcaggccaa
     3061 attgtctcga atacttgatg tggcccagga cttgaaggcc ttgtcaatgc tgctaaatgg
     3121 tactccattt gcctttgtta ttgaccttgc tgcacttgct tcacgtcgtg aatacctcaa
     3181 acttgataag tggctcacag ataaaattcg agagcatggg gagcctttta tccaggcgtg
     3241 tatgactttt ttaaagagac ggtgtccttc tattttgggc ggacttgccc cagaaaaaga
     3301 ccagcccaaa agtgctcaac ttcctccaga aactttggcg acaatgttgg cctgtctgca
     3361 agcttgtgca gggagtgttt ctcaggagct atcagaaact atcctcacca tggtagccaa
     3421 ttgcagtaat gttatgaata aggccagaca accaccacct ggagttatgc caaaaggacg
     3481 tcctcctagt gctagcagct tagatgccat ttctcctgtt cagattgacc ctcttgctgg
     3541 aatgacatct cttagtatag gtggttcagc tgcccctcac acccagagta tgcagggttt
     3601 tcctccaaat ttgggttctg cattcagtac ccctcagtca ccagcaaaag catttccacc
     3661 cctttcaacc cccaatcaga ccactgcatt cagtggtatt ggaggacttt catcacagct
     3721 tccagtaggt ggtcttggca caggcagcct gactggtata ggaactggtg ctcttggact
     3781 ccctgcagtg aataacgacc cttttgtaca gaggaaactg ggcacctctg gactgaatca
     3841 gcctacattc cagcagacgg acttgtctca ggtgtggcca gaggcaaacc agcactttag
     3901 taaagagata gatgatgaag caaacagcta tttccagcga atatataatc atccaccaca
     3961 tccaaccatg tctgttgatg aggtattaga aatgctgcag agatttaaag actctactat
     4021 aaagagggaa cgagaagtat ttaactgtat gctaaggaac ttgtttgaag aatatcgttt
     4081 ttttccccag tatcctgata aagagttaca tataacagcc tgcctatttg gtggtataat
     4141 tgagaaagga ctggtcactt acatggcact aggtctggct ctacgatatg ttcttgaagc
     4201 cttacgcaag ccttttggat ccaaaatgta ttatttcggg attgctgcac tagatagatt
     4261 taaaaacaga ttgaaggact atccccagta ttgtcagcat ttggcttcta tcagtcactt
     4321 tatgcaattt ccacatcatt tacaggagta tattgagtat ggacagcagt ctagagatcc
     4381 tcctgtgaaa atgcaaggct ctatcacaac ccctggaagt attgcactgg ctcaggccca
     4441 ggctcaggcc caggttccag caaaagctcc tcttgctggt caagttagca ctatggtaac
     4501 cacctcaaca actaccactg ttgctaaaac ggttacggtc accaggccaa ctggagtcag
     4561 ctttaagaaa gatgtgccac cttctattaa tactacaaat atagatacgt tgcttgtggc
     4621 cacagatcaa actgagagaa ttgtggagcc cccagaaaat atccaggaga aaattgcttt
     4681 tattttcaat aatctctcac agtcaaatat gacacaaaag gttgaagagc taaaggaaac
     4741 ggtgaaagaa gaatttatgc cttgggtttc acagtatctg gttatgaaga gagtcagtat
     4801 tgagccaaac tttcatagcc tgtattcaaa cttccttgac acgctgaaga atcctgaatt
     4861 taacaagatg gttctgaatg agacctacag aaacattaaa gtgctcctga cctctgataa
     4921 agctgcagcc aatttctcag atcgttcttt gctgaagaac ttgggacatt ggctaggaat
     4981 gatcacatta gctaaaaaca aacccatctt acacactgac ttggatgtga aatcattgct
     5041 gctagaggct tatgttaaag gacaacaaga attgctctat gtagtgccct ttgttgccaa
     5101 agtcttagaa tctagcattc gtagtgtggt ttttaggcca ccaaaccctt ggacaatggc
     5161 aattatgaat gtattagctg agctacatca ggagcatgac ttaaagttaa acttgaagtt
     5221 tgaaatcgag gttctctgca agaaccttgc attagacatc aatgagctaa aacctggaaa
     5281 cctcctaaag gataaagatc gcctgaagaa tttagatgag caactctctg ctccaaagaa
     5341 agatgtcaag cagccagaag aactccctcc catcacaacc acaacaactt ctactacacc
     5401 agctaccaac accacttgta cagccacggt tccaccacag ccacagtaca gctaccacga
     5461 catcaatgtc tattcccttg cgggcttggc accacacatt actctaaatc caacaattcc
     5521 cttgtttcag gcccatccac agttgaagca gtgtgtgcgt caggcaattg aacgggctgt
     5581 ccaggagctg gtccatcctg tggtggatcg atcaattaag attgccatga ctacttgtga
     5641 gcaaatagtc aggaaggatt ttgccctgga ttcggaggaa tctcgaatgc gaatagcagc
     5701 tcatcacatg atgcgtaact tgacagctgg aatggctatg attacatgca gggaaccttt
     5761 gctcatgagc atatctacca acttaaaaaa cagttttgcc tcagcccttc gtactgcttc
     5821 cccacaacaa agagaaatga tggatcaggc agctgctcaa ttagctcagg acaattgtga
     5881 gttggcttgc tgttttattc agaagactgc agtagaaaaa gcaggccctg agatggacaa
     5941 gagattagca actgaatttg agctgagaaa acatgctagg caagaaggac gcagatactg
     6001 tgatcctgtt gttttaacat atcaagctga acggatgcca gagcaaatca ggctgaaagt
     6061 tggtggtgtg gacccaaagc agttggctgt ttacgaagag tttgcacgca atgttcctta
     6121 aggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
     6181 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     6241 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     6301 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     6361 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     6421 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6481 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6541 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6601 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6661 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6721 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6781 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6841 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6961 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     7021 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7081 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7141 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7261 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     7321 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     7381 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     7441 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     7501 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     7561 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     7621 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     7681 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     7741 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     7801 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     7861 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     7921 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     7981 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     8041 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     8101 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     8161 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     8221 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8281 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8341 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     8401 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     8461 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     8521 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     8581 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     8641 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8701 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8761 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8821 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8881 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     8941 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9001 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9061 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9121 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9181 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9241 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9301 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9361 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9421 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
