LOCUS       sequence-290030-        8243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup_1-417-CG32016-E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8243)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 8243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup_1-417-CG32016-E_T
FEATURES             Location/Qualifiers
     source          1..8243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5528..5569
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5579..5596
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5619..5636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5669..5803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5693..5712)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5747..5766
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6425..7013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6505..6524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7184..7975,7976..8044))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7807..7826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8045..8149)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8217..8235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggaccct atgaaagagg aggcgatcga
     2761 attgaatggc catgctatgc aaatggccga agctgagcaa gaaaatggcg ctggcgccct
     2821 gaaaattgcc accaatgcgg gtgcaaccga tcgtccagcg catcagcagc ttccgctgcc
     2881 agtcgaagat cagcaggatg aggtgctgac ccccgctgag aagggcaagt tcgagtaccc
     2941 gccccctcca ccgccaccaa ctccagtcca ggctccgcta gccaccaagg ccaccgctct
     3001 gaatgccagt caagagcatg acgacgacga ggccaactcc gagaagtggg aggatccgtg
     3061 cgctccgccc ccgcctcctc cgctgccgac tagcgctttc ctggccaccg gactgggcta
     3121 cctcaagctg cccgccttca agctgaagga cgcactggag aaggccatca ccaagttgga
     3181 ggccaacaag cggacgctaa aggccagccc tgagtcttct cgttccatca aaaacaagaa
     3241 tgtggtggcc ctggagatgt tacccaggcg ctcaaaccca gaaacgatcg gcgatggatc
     3301 catgttggca tccacatcca cggctgttat gctgcagacc aaaaagcccg ccgttattgt
     3361 ggagatggag aggcgttgta agatcatcaa tttactggcc aaacagaatc aaatcctcga
     3421 atccatttcg ggggaggcca ttccgatgca tggaccctcg aagcatctgc acgaggacga
     3481 gggtctgacc ctgcaagttc tctcggcccg agcatccaca ccgtacacac agccctccag
     3541 catgttgtcc tgcacggccg ttagttgtga cctggagcac gattcgccgc ggaagcaagt
     3601 ggccagcaag gaggccgtgc cggagcagca gtctagtcag gtgcagcaaa agcgtccacc
     3661 cagcacaggc attcacaagc caggaagtct gcgagcaccc aaggctgttc gtcctactac
     3721 tgctccggtg gtcagcagca agccggtcaa gagctatacc cgctcccgcc tgatggacat
     3781 tcgcaacggg atgttcaatg ccctgatgca caggtccaag gagagctttg tgatgcctcg
     3841 catcgccacc tgcgacgaca ttgagttgga gggaaggctt cgccgcatga atatttggcg
     3901 cacctcggac ggcacaagat tccggacccg ctccaccaca gctaacttaa acatgaacaa
     3961 caacaacaac aatgagtgca tgttaattaa tatgataaaa gtggatatga ggactgattt
     4021 aataaatcca atagcagggt cattatgtca gacagtgcaa atggaaaaac ctattgctag
     4081 agacactgag gcattcaaaa aacttttgca acagttgggt tctcaagcca ggcagcatca
     4141 tccatgcaac gacgattgtc gtacaataaa cctctcaaat atagcaaatc atgtacacct
     4201 tgagtcaaaa ttacatcaaa aaataaatga tggtcatttg cagcaaccag agttaagtgt
     4261 taacgtgcca accatgccaa ctagtagtca tgtatttttg caaaagcgtt tggaaatcca
     4321 acatttaatt caaagacttc gttgtggaga cgtttcacat gattttttgg aaaaagagtt
     4381 ggataatccc agcaccccag cagccacaaa ggatgtcatt gccacagtgt tgagtgaata
     4441 ttctcattct aaaagaaatc cggtggtcac aggcgatccc aatattttta cccaacaatc
     4501 gtttcttcag cctcaatcag tccatcagca ctactctcaa gaattgcatt cgcaaaatac
     4561 tgctaaccat acgattaatc agctaatttc ccacggtaac tctccaactc cgttggcatt
     4621 tactcctact tcagttttaa gaaaaatgac agccgataag gatacgcaat caccaagcac
     4681 ttattgtcag aacccacaat atcatgtaca ccaacaaaat gccaagcaag tcggtactcg
     4741 tgagaacgta cttgagccac agctgaccgc aacaatggct gtacagcccc gaatgatatt
     4801 aggcggtgga aactttgcca ttggccaaaa caatcaacat ttgtctccca acatgtcaca
     4861 aagtcgtaat caacaagtgt taaagtggac ttctggcaat atgcaaatgg tccacggaaa
     4921 aacatttggc cgacatattc tgaaaggagg tcttaattca atgccgcatt ccaattctgc
     4981 gcttcccttt actgcccata aaattgaaat gcaacccatt catcaaccac atctacagca
     5041 gcagcaacat agatttaaag cagttcaatc tgtggaatca aatctgaata ccgaaagtgt
     5101 tcaccaaaac attacatctc ctgttggatg gcaccaactt tacatgcaac atcagcaaca
     5161 acatcatcac acacgacagc agcttagtca aagagtgatt tatggagaaa tgcaccgtca
     5221 atcaaatcca caaatgtctc cgccagtccc aggtttttct gacagctccg attccggtaa
     5281 tgtgatcaaa gctaacagcc tcacatctcc tagctaccag agagatgaga ggatttcatc
     5341 accaaccaat caattggcgc aatggttttc ccctgagctt cttgcaaaag cctctgctgg
     5401 aaaactgcca cttttaaatg tgaaccaagc actcagcttg gaagaatttg aacgaagcat
     5461 tcaacattcc tcaggcgtcg tgcataacta aggcggccgc tcgagtctag agggcccgcg
     5521 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     5581 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     5641 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     5701 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5761 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5821 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     5881 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     5941 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     6001 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     6061 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     6121 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     6181 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     6241 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     6301 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     6361 cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6421 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6481 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6541 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6601 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6661 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6721 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6781 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6841 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6901 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6961 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7021 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7081 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7141 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7201 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7261 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7321 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7381 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7441 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     7501 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7561 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7621 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7681 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7741 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7801 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7861 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7921 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7981 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8041 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8101 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8161 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     8221 ggcgtatcac gaggcccttt cgt
//
