LOCUS       sequence-290032-        9344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCYFIP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCYFIP1_T
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2679..2705
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(3889..3900)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             6629..6670
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6680..6697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6720..6737)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6770..6904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6794..6813)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6848..6867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7526..8114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7606..7625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8285..9076,9077..9145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8908..8927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9146..9250)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9318..9336
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccac atatggctta cccatacgat gttccagatt
     2701 acgctcctcc aagcttaatg acggagaaga ttacgctagc cgacgcgctg tccaacgtgg
     2761 aggtgttgga cgagctatcc ctgccggacg agcagccctg catcgaggcg cagccctgct
     2821 cgattatcta caaggcaaac ttcgatacga atttcgagga tcgcaatgga tttgtcacgg
     2881 gtatcgccaa gtacatcgag gaggccacca cccatgccaa cttgaatgtg ctcctggacg
     2941 aggggcagaa acacgcagta atgctctaca cctggcgctg ctgctcgcgc gccattccgc
     3001 agcccaagtc caatgagcag ccgaaccgcg tggagatcta cgagaagacg gtcgaggtgc
     3061 tagccccgga ggtgaacaag ctgctcaact tcatgtactt ccaacgcaag gccatcgagg
     3121 ccttctccgg cgaggtgaag cgcttgtgcc acgtcgagaa gcgcaaggac ttcgtgtccg
     3181 aggcgtacct attgacgcta ggcaagttta ttaacatgtt tgccgtgctg gacgagctga
     3241 agaacatgaa gtccagcgtg aagaacgatt actccaccta caggcgagct gcgcagttcc
     3301 tcaaagtgat gtcggactcg cacactttgc aggagtcgca gaatctgtcc atgttcctgg
     3361 ccacgcagaa caagattcgc gacacggtca aggacacgct ggagaagatc gtgggctacg
     3421 aggatctgct ctcagatgtg gtcaacattt gcgtgcatat gttcgagaca aagatgtact
     3481 tgacccctga ggagaagcac atgctggtaa aagttatggg ctttggcctg ttccttatgg
     3541 acagcgatgc gtgcaatatc aacaaacttg atcaaaagaa gaagatacgg ctggatcgca
     3601 tcgatcgcat ctttaagaac ctggaagtgg tgcccctctt cggcgacatg cagattgctc
     3661 ccttcaacta cataaagcgc agcaagcact tcgattccag caagtggccg ctgtccagct
     3721 ctaacgccat cagcccacag gcggatctaa tggtgcattt gccgcagata cgcgaggatc
     3781 acgtcaagta catttcggag ctggcgcgat acacaaacga ggtgaccacc accgtcaagg
     3841 agaatccctc tgatgccgag aaccggatta cggccgacct ggctctgcgc ggccttcaat
     3901 tgctgtccga gtggaccagt gtggtcaccg agttgtactc ctggaagctc ctgcatccga
     3961 ctgatcatca ccagaacaag gagtgccccg tggaggctga ggagtacgag cgggccaccc
     4021 gctacaatta cacctcggaa gagaagttcg cgctgatcga ggtcatcgcc atgataaagg
     4081 gactgcaggt gctaatggcg cgcattgaaa ccgtattgtg cgaggctatc cggcgtaaca
     4141 tctattccga gctgcaggac ttcgtgcagc tttcgcttcg tgaaccgctg cgaaaggccg
     4201 taaaaaataa gaaggatctt atccggagca tcatcatgtc ggtgcgagag acatcggcgg
     4261 actggcaaaa gggctatgag cccaccgacg atccagtggc caagggcaag aaggatcccg
     4321 acggcggctt ccgcatccag gtgcctcgcc tcaatgtggg accttcctcc actcagctat
     4381 acatggtgcg caccatgctg gagtctctga ttgccgacaa aagcggcggc aagcgcactt
     4441 tgcgcaagga catcgacgga aactgtttgc tgcagatcga cacgttccac aagacatcgt
     4501 tctactggag ctacttgctc aacttcagcg acacgcttca gaagtgctgc gatctctcgc
     4561 agctgtggta tcgcgagttc tacttggaga tgaccatggg tcgtaaggtt aacaagtgct
     4621 tggtgcggca tcagcacaac gaggagtgca aggatctgat caccatggag aagcgcattc
     4681 agtttccgat tgaaatgtcc atgccctgga tcctaaccga tcacattctg caaacgaagg
     4741 agccctcaat gatggaattt gtcttgtatc ccctggactt gtacaacgac tcggcgtact
     4801 acgcactcac tgtcttccga aagcagttcc tgtacgacga agtggaggcc gaagtcaatc
     4861 tgtgcttcga tcaatttgtc tacaagctga gcgagcagat ctttgcccat tacaagcaat
     4921 tggcgggcag catcttcttg gacaagcgat tccgtttgga gtgcgaggta ctcggtttca
     4981 acttccagtc gtatccccgc aataatcgtt atgagacctt gttaaaacaa cgacatgtcc
     5041 aacttttggg ccgttctatt gacttgaaca aactgatcac acaacgcata aacgccaaca
     5101 tgcacaagag cattgagttg gccatcagtc gtttcgaggg caacgatata accggcattg
     5161 tggaactcga aggcctgcta gaggcgaatc gaatttgcca caagttgcta agcaagtact
     5221 tggccctgga taactttgac ggcatggtaa aggaggctaa ccacaatgta ctggcgcctt
     5281 acggcagaat aacactgcac gtctttgtgg aattgaacta cgactttctg gtcaattact
     5341 gctataatgc agccacgaat cgtttcattc gaaccaaagt gaatctttcg tcgtcacagg
     5401 ctattcagcg tgagaagccg ccacaaatgt cccattacta tctgtggggt tccaagcagc
     5461 tgaatgctgc ctattccaca cagtatggcc agtacactgg ctttgtaggt tcaccgcatt
     5521 tccatgccat gtgccgcctg ctgggctatc agggcatagc agtcgtcatg gacattatac
     5581 tgaaggacat tgttaagcca ctgatccagg gctcgctgct gcagttcacc aagacgctga
     5641 tgattgccat gcccaagtca tgcaaactac cccgttgcga atatggttcg cctggagtgc
     5701 tcagctacta tcaggctcat cttacagaca tcgtgcaata tccggacgcg aagacagagc
     5761 tatttcagtc tttccgggag tttggcaaca gcattatctt ttgcctgctg atcgaacagg
     5821 cgctctcgca ggaggaggtg tgcgatctac tgcacgccgc cctcttccag aacatctttc
     5881 ccagaccttt ttgcaaagag aatgaaaaac cggaggctaa gcaaaagcgt cttgaagcac
     5941 agtttgccaa tctgcagata gtttcaaacg tggaaaagat tggcactgca aagcaagcaa
     6001 tgatagcacg agagggagat ttgctgactc gtgagcgtct ttgctgtggc ctcagcattt
     6061 ttgaagttat tctcaaccgc gtgaagagct atttggacga ccctgtgtgg tgtggtcctc
     6121 caccggccaa cgggattatc cacgtggatg agtgctcgga gttccatcgc ctttggtcgg
     6181 ctctgcagtt cgtttattgc attccagtca ggggaacgga gtacaccatc gaggagctct
     6241 tcggcgaagg tctgaactgg gctggctgcg tcatgatcgt actgctggga cagcagcgtc
     6301 gcttcgaggc tctggacttt tgctaccaca ttctgcgggt acagcgcgtc gatggcaagg
     6361 atgaggacgt aaagggcatt caattgaagc gaatggtgga tcggattcgt cggttccaag
     6421 tattaaactc acaaatcttc tccattctca acaagtatct aaagggcggc gatggcgagg
     6481 gctcaaatgt ggagcacgtg cggtgttttc caccgcctca gcatccctcg gtcatatcgt
     6541 cctcgtcgca ctaccaggat ccccagaagc tgcggcagag cataaacaat tgagcggccg
     6601 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     6661 cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
     6721 ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat
     6781 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6841 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6901 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6961 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga
     7021 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     7081 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     7141 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     7201 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     7261 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     7321 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     7381 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     7441 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag gccaggaacc
     7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8581 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8881 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9241 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9301 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgt
//
