LOCUS       sequence-290038-        7993 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmThor-DmCup_417-1133.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmThor-DmCup_417-1133_T
FEATURES             Location/Qualifiers
     source          1..7993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5278..5319
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5329..5346
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5369..5386)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5419..5553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5443..5462)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5497..5516
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6175..6763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6255..6274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6934..7725,7726..7794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7557..7576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7795..7899)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7967..7985
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgtccgct tcacccaccg cccgtcaagc
     2761 catcacccag gccctgccca tgatcaccag gaaggttgtc atctcggatc cgatccagat
     2821 gcccgaggtg tactcctcga cgcccggcgg aaccctctac tccaccactc ctggaggcac
     2881 caaacttatc tacgagcggg ctttcatgaa gaatctccgt ggctccccat tgagccaaac
     2941 tccgccgtcc aacgtgccca gttgcttgct gaggggcact ccgcgtactc ccttccgcaa
     3001 gtgcgtgccc gtccccacgg aactgatcaa gcagaccaag tcgctgaaga tcgaggacca
     3061 ggaacagttc caactggatc tgttaattaa tatgcctgcc ttcttcaaaa acaaaaataa
     3121 gccgaaccta atcagtgacg agtccatcat ccagagccaa ccgccccagc cgcaaacgga
     3181 gttccaagat ccggccattg ttaaccaaag acgcattggc agcggtcgct taaaccattc
     3241 caagtggggt tacaacgacg aagactacca ctcgtaccac aatggcaagt cgcagcatat
     3301 ggaggaggtc aactcgaaga acagcaaaaa catgacggtg ctgcagttct tcgacaacgg
     3361 cgagatcagc agtcagcctc aaagacggcc gaacactcct gtgatgggga tgtctatcaa
     3421 tcgatccgag aacgacaccc tgcactcgaa cgagtccagt gaagatttga gtcgcgccaa
     3481 cgagaactat gtaaagcgcg taatgtctgg attcctggtt gtatcgaagc ccaagtcacg
     3541 agatgtcgag gatagacatc accggcggta caggaaccag aacgaggagc ctgagtggtt
     3601 tagctgtgga cccacgtcta gactggacac tatagagttg tgtgggttcg atgaggacga
     3661 ggagaaaatg ctaaaggagg gcaacaaaaa ccacggatta ggcgaaacag agcgagaaac
     3721 gtcaaaacaa aagatggacc ataagtacaa atggacgcat gcggagccga tgggtcgaag
     3781 caaatacatg cccaaacacg acactaacaa taatcataac gtggagaaca tgaataatgt
     3841 tatggctacc gagcatcagc aacaaaagga agagaaacgc ccaggcagtg gccgttcgtt
     3901 tcagtttgat aagttcaatc aaagccaaca gaattacgag agtagcagtt atgttaatca
     3961 ccaacagccg ccgcaaacgc agcctcagca aatgcagcag caatcgaaca caaacacgaa
     4021 caactctaaa ttcatgtcat tctttgccaa tgaggggaac tcctcgtctt cttcgctgaa
     4081 cgagtttttc aagcaggcca ttaaccaggg tcatggcaac aatccggagc agcccaaatc
     4141 gttgggccac atcggacaga tgccttcggt ggatcagctg gaggccaaat ggcgtcgaaa
     4201 ctcactgaac aatgttggcg agactgccaa caagcagact gataacttcc agaagctaat
     4261 tgggtctctt tcttcggcta agccacagtc acaggcggtt ggctatgatg ccatctccaa
     4321 ctttataatg cagcaacagc agtaccagca gcagcaacag aagcagcacc tcatcataca
     4381 gcagcagcag caacagaccg cctttttggc gagcctgcag ctgaaggcga tcctcggccg
     4441 ggccgacacc caattgctac tgctccgtct gactaaaggg gaaatatcca agcacggatt
     4501 gctggtgcag ttggccaacc cacgcttgac ggacatggat cgcgaggcca tcactgctgt
     4561 attgcagttc accaacaccc agcagcagca gcagcagcac aagcagcagc tggacatgct
     4621 ctcaagcacg gtcatcgcca gccagttgca gaatctccac aacttggcca ttgtccagca
     4681 gactcttgca gccaggcagc agccgcagca taatccacag acgcaggcgc cgcaccaact
     4741 gtcgcaggag gacttgcaag cgcatgccaa tgtgataatg cgaaacgccg taatgaagcg
     4801 aaaaattgag gagcagacct cgaagcttat caatggcggt gccaagcatc aggcacagca
     4861 gcaatatcta aaccgtggcc agcaacgcca ggctcgacca gatgccaact cgaatgcctt
     4921 gctccatgcc ttgatctctg gcggtggcaa taaccacgcc tctggttatc ctatgaaagg
     4981 acagccgcag cagcatcatt ccaatttgag attcggtgat aaccaaaact tccaatcttt
     5041 tgagtccaac cagccgcact tcgccacgca gtacaagcag caataccaac agtctcagca
     5101 gcaacatccc catcagcagc cccaacagct gaattcgctt catcagaaca acgctggagc
     5161 tgtcaatagc ttcaataagg cccaaatgca agctcaatca gctatttcca tgctgcccaa
     5221 cagcggggat gagtttcatt aagcggccgc tcgagtctag agggcccgcg gttcgaaggt
     5281 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca tcatcaccat
     5341 caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc
     5401 tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt tggacaaacc
     5461 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5521 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5581 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5641 ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc cagtcagctc
     5701 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat
     5761 gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg
     5821 ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc
     5881 tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc
     5941 cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat
     6001 ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc
     6061 catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc
     6121 tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     6181 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     6241 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     6301 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     6361 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     6421 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     6481 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     6541 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     6601 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     6661 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     6721 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6781 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6841 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     7021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     7081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     7141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     7201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     7261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     7321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     7381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     7441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     7501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     7561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     7621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     7681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     7741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7921 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7981 gaggcccttt cgt
//
