LOCUS       sequence-290039-        8775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmThor-DmeIF4Ga_687-1666.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmThor-DmeIF4Ga_687-1666_T
FEATURES             Location/Qualifiers
     source          1..8775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6060..6101
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6111..6128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6151..6168)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6201..6335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6225..6244)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6279..6298
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6957..7545)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7037..7056)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7716..8507,8508..8576))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8339..8358
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8577..8681)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8749..8767
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catgtccgct tcacccaccg cccgtcaagc
     2761 catcacccag gccctgccca tgatcaccag gaaggttgtc atctcggatc cgatccagat
     2821 gcccgaggtg tactcctcga cgcccggcgg aaccctctac tccaccactc ctggaggcac
     2881 caaacttatc tacgagcggg ctttcatgaa gaatctccgt ggctccccat tgagccaaac
     2941 tccgccgtcc aacgtgccca gttgcttgct gaggggcact ccgcgtactc ccttccgcaa
     3001 gtgcgtgccc gtccccacgg aactgatcaa gcagaccaag tcgctgaaga tcgaggacca
     3061 ggaacagttc caactggatc tgttaattaa tatgaaacag atgtcgatgt ctggcgtaca
     3121 aagtggcggc ggtagaagca gtatgaaggg catgatacac gtaaatttat cattaaatca
     3181 agatgtaaag ttaagtgaaa atgaaaatgc atggcgccct cgggtcttaa ataaatctga
     3241 tggtgatagc gacgcgaaat ctgcactcga aaaagatgaa ttggtacgtc gtgttcgggg
     3301 tattttaaat aagcttaccc cagaaaggtt tgacacactt gttgaggaga ttataaaatt
     3361 gaaaattgat acacccgaca aagtggacga agtaattgtg ttggtatttg aaaaggccat
     3421 tgatgagcca aacttttctg tgtcttacgc cagactatgt caacgacttg ctgcggaagt
     3481 gaaggtaata gacgagcgca tggaaagcga aaccaaatct aactcggcac actttcgaaa
     3541 tgcattgtta gataaaactg aacaggaatt cacccaaaac gtttcacaaa gcaccgcaaa
     3601 ggaaaaaaaa ttacagccaa ttgtggataa aattaagaaa tgtactgatg ccaatgaaaa
     3661 agcggaactt gaggcatttt tggaagaaga agaaagaaaa ataagaagac gatccggagg
     3721 gacagtacga tttattggcg aactttttaa gatatctatg ttgactggca aaataatata
     3781 ctcttgcatc gatacacttc ttaaccctca ttctgaagac atgcttgagt gtttgtgcaa
     3841 gctgcttaca actgtgggtg ctaaatttga aaagacccca gtaaattcca aagaccctag
     3901 tcggtgttat tcgttggaaa aatcgatcac caggatgcag gccatcgcat cgaaaactga
     3961 caaagatgga gccagagtaa gctcaagagt acgatttatg cttcaagacg ttatcgactt
     4021 gcgtaagaat aaatggcaaa cttcgcggaa tgaagcaccc aaaactatgg gacaaattga
     4081 aaaagaggca aagaatgagc agctttctgc acaatatttt ggtacacttt ccagtactac
     4141 acccggagga tcgcagggcg gctctggtaa acgagatgac cgtggaaact ctcgatacgg
     4201 tgaatctcgt tcaagtagcg cgtatggtgg cagccacagt caacgtggtg ataatggcaa
     4261 ccttcgacac cagcagcaga acaacgtcgg gggaaacgtc tctggaggtg ctggacactc
     4321 caatggaaac aatgatgaaa atacttggca tgtgcaaacc agcaaaggta gccgctctct
     4381 agcggttgac agcaataaac tggaaggcct gtctaaactt tctgaccaaa atctggaaac
     4441 aaagaagatg ggtggattaa cgcaattcat ttggattagt agtgacacga caagactatc
     4501 gtcggcgccg acaccaaccc catccaatcc tttcgctgtg ctctcatccc taattgataa
     4561 aaatagtaat gaaagggatc gcgatcgcag tggacccaga aacaaaggat catacaacaa
     4621 aggttctatg gagcgggatc gttatgatag aggtatgcat tcgagaactg gatcatctca
     4681 gggatcacga gaaaactcat cctcccgcgg tggtcagcag ggtcgaactt tattgagttc
     4741 atcagttcaa aaatctacta gtcattcaaa atacacccaa caggcaccac ctacacggca
     4801 tactgttaaa gcccagtcat cagtgggcag ctctaacgtt aacacaggtc cactctaccg
     4861 tggaagtgag cagcaaacat cagcaacctt ctcgcagacc acccgttctg ttgctcctgt
     4921 agcagtgttc atcgaagcta gcgaaacaga tttaaaactc atcaaatctg ttgtttcaga
     4981 aattgtagac ctatcagctg catctaaaga agtcacgccc ggtgcagttt cgtgcataaa
     5041 acgagtacca gagaaactac ggtgcagttt catttattat attttaacag attatttgca
     5101 tttagcaaat gtaggtaaac agtacaggcg gtatttatct attgccgtct cccaacttat
     5161 tcagcagaat tacatttctg ccgatcattt gagactggct tacaacgaat ttaccgtata
     5221 tgcaaacgat ttgattgttg atattccgga gctttggcta tacattcttc aatttgccgg
     5281 gccactaatt gtaaagaaaa tattgactat atcagatttg tggaacaaca atttaaaaga
     5341 aaatagtcca tcaaacgtcg ctaaaaagtt ccttaaaaca tacttgatat attgcacaca
     5401 agaagttgga ccaaattttg ctcggaatat gtggatcaag tttaacctaa aatggtcaga
     5461 ttttatgcct gaaagtgaag ttgccgactt tattaaattt aacagactgg aatatgtgga
     5521 aaacgaatca aagtcgccag tgattgatca tagagagact ccagaaaagc acgttaaaaa
     5581 tgtgatagac catattgagc acttactaaa ggaaggaaca acggcggact gcattattga
     5641 ttatagtaac ggaaatataa tggtggttga taagttgttt atcagaggat tgacggaaac
     5701 attgagtaat ttctcgattc actacaagga caacagttac aagcttgagt ccgaaacctt
     5761 tcaaaagttt tgcataccgg ttttgcagag atacattgat tctaatgaag accaccaact
     5821 tgaatgttta tatactttgc agcttttagt tcatggattg gagcatccaa gagggttgct
     5881 gagtgaacta attggcgagc tatatgatgc ctttgtaata cagaaggaat cgctttgtaa
     5941 gtggcgagat tcaaaggatc agtctgctgg caagggcgtt gctgtaaaaa gtcttaatcc
     6001 tttttttaac tcgttgttaa acgatgatgc caacagttct agagggcccg cggttcgaag
     6061 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     6121 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc
     6181 gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa
     6241 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     6301 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     6361 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     6421 gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc
     6481 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     6541 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     6601 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     6661 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     6721 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     6781 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     6841 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     6901 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6961 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7021 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7081 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7141 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7201 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7261 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7321 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7381 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7441 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7501 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7561 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7621 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7681 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7741 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7801 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7861 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7921 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7981 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     8041 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8101 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8161 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8221 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8281 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     8341 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8401 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8461 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8521 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8581 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8641 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8701 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8761 acgaggccct ttcgt
//
