LOCUS       sequence-290057-        8372 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of Dm4E-T-del321-356.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-Dm4E-T-del312-356_T
FEATURES             Location/Qualifiers
     source          1..8372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5657..5698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5708..5725
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5748..5765)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5798..5932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5822..5841)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5876..5895
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6554..7142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6634..6653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7313..8104,8105..8173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7936..7955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8174..8278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8346..8364
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatggatac atcaaagatt agtgccaggt actcgaaagt
     2761 agacctgtta gctctaagat atgaaggtaa aagtcgacag cgcccgcaat gttcaacacg
     2821 acttgaattg cagacgctag gtttttggaa aattaatctg aacacagctg cgttaaccgt
     2881 gtccagcgcc tattctaatc aaaacaagaa tcgactttct ccagaagcag ataactcgag
     2941 cttaatttgt tccaatagta gctcaataag ttcacgtcgc gctatgagaa atcgagaacg
     3001 ggcaaacaac tattaccagc gcttcgtacc taccgattct cttctaataa gtggggagga
     3061 taaagataag gatgcgcttt ctcatggaca gccctataag tcgaatatca tcgatcaccg
     3121 tagcatatca tcttcacatt taatgccggc atttgcaaag aggcgttttg tcatatcgaa
     3181 aggcagcaac agcgaggaaa gcaacgaggg gataaacact tgtgcaagca agggcaaggc
     3241 agccagttcg cctagccgta agggtagcga gttggatact gctgagacat gtttgaattt
     3301 tgttcagccg gatcacgacc aatgcatgtc atcttcacct acattttcaa catcccgcca
     3361 agaaaggcgg attggtagcg gtcggctgtt gccaagaagt gataattggg attacaaaaa
     3421 cgaaaagacc gtagaagcca gcattgagaa tgaaaaggaa acatctccaa atggatctgg
     3481 aagtacaagc agtcttaatc agcacaatca aagccaacat cgttctcgaa cattcagtgg
     3541 aaggttagtt gaacgtgttc ccgaagtcac agatcgtcgt tttcaatatg actccaaaaa
     3601 gtcttttgat cgtcagggaa ttaataatcg acgcatttct ggtaaggagc ctttccgatc
     3661 cgtaactgaa gataaaaata atcaaattca acaacttgat aaaaatttag acgcgcaagc
     3721 aagcaaagat gaagcttcga tgagaaacag caatgatagt ttaaatttcc gcgaagtcat
     3781 tccaagtgat gaaaagaagc acactgacga aaatgtagtg acttctattc aaaactcaac
     3841 agatctcggc catcccaata aaaacaaacc aattcagatg cagcccagtc agaatcctga
     3901 aagtgaattt aattttgatg cctttcttaa tatgcatccc ttggataatt ccgttttgag
     3961 taatgatgaa actggaaaaa gtgactcaaa aggaacatcc cgttttagcc gatggtttcg
     4021 acaaaaagaa gctgcaaata ataatgagtt tccagggttt cgggaatcgc atgctcaaga
     4081 aaaacgtggt attccaagtg ttaaggatct ggaagctcaa atgataaaag tggatatgag
     4141 gactgattta ataaatccaa tagcagggtc attatgtcag acagtgcaaa tggaaaaacc
     4201 tattgctaga gacactgagg cattcaaaaa acttttgcaa cagttgggtt ctcaagccag
     4261 gcagcatcat ccatgcaacg acgattgtcg tacaataaac ctctcaaata tagcaaatca
     4321 tgtacacctt gagtcaaaat tacatcaaaa aataaatgat ggtcatttgc agcaaccaga
     4381 gttaagtgtt aacgtgccaa ccatgccaac tagtagtcat gtatttttgc aaaagcgttt
     4441 ggaaatccaa catttaattc aaagacttca ttgtggagac gtttcacatg attttttgga
     4501 aaaagaattg gataatccca gcaccccagc agccacaaag gatgtcattg ccacagtgtt
     4561 gaatgaatat tctcattcta aaagaaatcc ggtggtcaca ggcgatccca atatttttac
     4621 ccaacaatcg tttcttcagc ctcaatcagt ccatcagcac tactctcaag aattgcattc
     4681 gcaaaatact gctaaccata cgattaatca gctaatttcc cacggtaact ctccaactcc
     4741 gttggcattt actcctactt cagttttaag aaaaatgaca gccgataagg atacgcaatc
     4801 accaagcact tattgtcaga acccacaata tcatgtacac caacaaaatg ccaagcaagt
     4861 cggtactcgt gagaacgtac ttgagccaca gctgaccgca acaatggctg tacagccccg
     4921 aatgatatta ggcggtggaa actttgccat tggccaaaac aatcaacatt tgtctcccaa
     4981 catgtcacaa agtcgtaatc aacaagtgtt aaagtggact tctggcaata tgcaaatggt
     5041 ccacggaaaa acatttggcc gacctattct gaaaggaggt cttaattcaa tgccgcattc
     5101 caattctgca cttcctttta ctgcccataa aattgaaatg caacccattc atcaaccaca
     5161 tctacagcag cagcaacata gatttaaagc agttcaatct gtggaatcaa atctgaatac
     5221 cgaaagtgtt caccaaaaca ttacatctcc tgttggatgg caccaacttt acatgcaaca
     5281 tcagcaacaa catcatcaca cacgacagca gcttagtcaa agagtgattt atggagaaat
     5341 gcaccgtcaa tcaaatccac aaatgtctcc gccagtccca ggtttttctg acagctccga
     5401 ttccggtaat gtgatcaaag caaacagcct cacatctcct agctaccaga gagatgagag
     5461 gatttcatca ccaaccaatc aattggcgca atggttttcc cctgagcttc ttgcaaaagc
     5521 ctctgctgga aaactgccac ttttaaatgt gaaccaagca ctcagtttgg aagaatttga
     5581 acgaagcatt caacattcct caggcgtcgt gcataactaa ggcggccgct cgagtctaga
     5641 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     5701 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     5761 ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat
     5821 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5881 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5941 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     6001 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag
     6061 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     6121 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     6181 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     6241 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     6301 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     6361 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     6421 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     6481 ttcaaggatc gctcgcggct cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg
     6541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6721 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     6781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6961 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7621 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7921 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8281 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8341 ataaaaatag gcgtatcacg aggccctttc gt
//
