LOCUS       sequence-290105-        8437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN3-L676EG683E-del460-465.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
  TITLE     Structure of the PAN3 pseudokinase reveals the basis for 
            interactions with the PAN2 deadenylase and the GW182 proteins.
  JOURNAL   Mol Cell. 2013 Aug 8;51(3):360-73. doi: 
            10.1016/j.molcel.2013.07.011.
  PUBMED    23932717
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.07"; journalName: "Mol Cell"; date: 
            "2013-08-8- 8"; volume: "51"; issue: "3"; pages: "360-73"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN3-L676EG683E-del460-465_U
FEATURES             Location/Qualifiers
     source          1..8437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5722..5763
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5773..5790
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5813..5830)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5863..5997
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5887..5906)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5941..5960
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6619..7207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6699..6718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7378..8169,8170..8238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8001..8020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8239..8343)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8411..8429
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattcta tgtgctcgac ttatggactc ttatcttttg agttttcaga
     3361 tacggctgct atggatccaa ttttcttctc gccaacgaat ggaataccgt cagagagtaa
     3421 actcgccact tatatgaatc gacagaatgt cgccacaccg agcggttatg ggctgaataa
     3481 cgggttctca ctacttaatt tggattcgcc cctaaataaa aagtcccagg tcacgccgca
     3541 gtcgcccgag ttcattccaa cgcgccttgg ctcgacgccc aacttctatg caccatatca
     3601 caacgcgccc atgcaattaa gtaatgggct caatggcgtc aatgggttaa cagccgccgc
     3661 agcagcagca gcagcagccg ccgccgccgc cgcagcagca gttccctcct ccacgtcatc
     3721 ggcgtcctcc gcctcggtga ccatcagcaa aacggccaac ggtggcgcct ccatgctcgc
     3781 cctgcaaaag accgcatcca tcgcctctgt aaccatcgca caacagcagc cacagcatcc
     3841 acagaaacag caacagcatc cgccgtcggt gggcggagga gctgtgtccg ccgcctccgc
     3901 ccccattgcc attagtggcg ctccaccgaa tcccgccgca gctccgttcg tcagcagcat
     3961 gtccgctcag acgccgctca aggggagggg cgccatgatg cgccaggaat caccaacggc
     4021 agccatgatt tcgggcgcag ggccaggtga gaagtcgcca ccgcacggga tgacgccgca
     4081 cggagcatcg cccataccat cagcgctgcc caccagcgtg caccaggaga acgtaggcgg
     4141 caccatatac ttctatccga ctgccaacgc acagaacagc cagcccgtcg tcaattccat
     4201 ggtggtggat ggaacgcatc cggctctcca tggcgtgagt gctgtggcgc cgatgagtgc
     4261 gggcgttccg gcggccatga tgtacacggg gcatgtgtat ccgggaccgt cctccaacgt
     4321 ggtcaccatg cagccaaaga cgctgctgga gtcggccttc tttatgcccg acgagatgcg
     4381 cgccgaagtc ctcgcccgca acgagatctc taacctgatc atggacgcag cggaggctgc
     4441 gcagcacgct ctaccgctgg aggtggagaa ctaccatgcc ctgtatccac tggaaccgcc
     4501 ggcgcagccg ttgcacgcca agctcacgtt ccccgccacc acttataggg ccacgcacaa
     4561 cacgacgggc tacaagtact gcctgcgaag aatacatggt ttccgcctgc agtcgaccaa
     4621 gtgcatgacg ctggtggaga tgtggaagaa gctgcagcac acgaatgtgg tacaactgcg
     4681 tgaggtgttc acgacgggta gtagtggttc tttagtatta gtgtacgact atcatcccgg
     4741 ctcacaaaca ttactggcca aatacttcac tccggcgcca gagaccaacg ggtacactga
     4801 tccattccaa ggcgaggccc gccccttcag tcataagagt aacatgcagc gaacgagcaa
     4861 tggaccgctg ctaccggagg ccaccatctg gtcgatcatc atgcagctga ccgccggctt
     4921 aaaagccatc caccatgccg gtcttgcctg caaggttctg gatcccacaa agattatagt
     4981 gacgggcaag cgtgtacggt ttagctcatg ctgcatttcg gatataacgc aattcgatcc
     5041 gaatgcatcc aatcccttgg cgctggttaa tatgcatcag caggacgatc tgaccgcttt
     5101 gggtcgctta gtattagctt tggcctgccg ctgtcttcaa tccgtgcaac gcgacaatgt
     5161 ccagtccagc atcgatatgg tcacgcgaaa ctactccacc gatctgcgaa actttattgt
     5221 ttacctcttc accaccaata atcgtcgctc cgtgaccgac ctgatgccga tgattggtgc
     5281 ccgcttctat acgcaactgg atgcactgca gagcaaaatt gatatgcagg aggacgagga
     5341 agccaaggag atggagaacg agcggttata tcgcattttg gtcaaactta atagcatcaa
     5401 cgagcgaccc gactttaatc tggactgcac ttggtctgag actggtgata gatacatgtt
     5461 gaaattattc cgtgattatt tgttccattc cgtcactgag gatggccggc cctggctaga
     5521 tcacgcacac attgtacaat gcctcaacaa gctggatgct ggctccatag agcgcgtgca
     5581 actgatgtcc cgcgacgagc aatcggtact catcgtctcc tatgctgaac tcaagaattg
     5641 tctggagaac gccttctccg aactgatgtc aagtgcggcc aactgagcgg ccgctcgagt
     5701 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     5761 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     5821 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     5881 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     5941 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     6001 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     6061 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt
     6121 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     6181 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     6241 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     6301 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     6361 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     6421 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     6481 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     6541 acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa
     6601 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6661 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6721 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6781 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     6841 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6901 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6961 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7021 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     7081 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7141 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7201 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7261 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7321 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7381 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7441 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7501 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7561 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7621 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7681 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7741 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7801 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7861 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7921 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7981 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8041 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8101 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8161 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8221 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8281 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8341 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8401 aacctataaa aataggcgta tcacgaggcc ctttcgt
//
