LOCUS       sequence-290194-        9610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of NcQde-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9610)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pETM-60-NdeI-NcQde-2_V
FEATURES             Location/Qualifiers
     source          1..9610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5071..6552
                     /codon_start=1
                     /gene="nusA"
                     /product="transcription elongation protein (N utilization 
                     substance protein A)"
                     /label=NusA
                     /note="highly soluble in E. coli"
                     /translation="MKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDVR
                     VQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDRI
                     TTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVILR
                     EDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIEI
                     KAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPAQ
                     FVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMTV
                     DDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLDE
                     PTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLEDL
                     AEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     CDS             6568..6585
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             6595..6615
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9454..9471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9524..9542)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9538..9585
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgaaagaaa ttttggctgt agttgaagcc
     5101 gtatccaatg aaaaggcgct acctcgcgag aagattttcg aagcattgga aagcgcgctg
     5161 gcgacagcaa caaagaaaaa atatgaacaa gagatcgacg tccgcgtaca gatcgatcgc
     5221 aaaagcggtg attttgacac tttccgtcgc tggttagttg ttgatgaagt cacccagccg
     5281 accaaggaaa tcacccttga agccgcacgt tatgaagatg aaagcctgaa cctgggcgat
     5341 tacgttgaag atcagattga gtctgttacc tttgaccgta tcactaccca gacggcaaaa
     5401 caggttatcg tgcagaaagt gcgtgaagcc gaacgtgcga tggtggttga tcagttccgt
     5461 gaacacgaag gtgaaatcat caccggcgtg gtgaaaaaag taaaccgcga caacatctct
     5521 ctggatctgg gcaacaacgc tgaagccgtg atcctgcgcg aagatatgct gccgcgtgaa
     5581 aacttccgcc ctggcgaccg cgttcgtggc gtgctctatt ccgttcgccc ggaagcgcgt
     5641 ggcgcgcaac tgttcgtcac tcgttccaag ccggaaatgc tgatcgaact gttccgtatt
     5701 gaagtgccag aaatcggcga agaagtgatt gaaattaaag cagcggctcg cgatccgggt
     5761 tctcgtgcga aaatcgcggt gaaaaccaac gataaacgta tcgatccggt aggtgcttgc
     5821 gtaggtatgc gtggcgcgcg tgttcaggcg gtgtctactg aactgggtgg cgagcgtatc
     5881 gatatcgtcc tgtgggatga taacccggcg cagttcgtga ttaacgcaat ggcaccggca
     5941 gacgttgctt ctatcgtggt ggatgaagat aaacacacta tggacatcgc cgttgaagcc
     6001 ggtaatctgg cgcaggcgat tggccgtaac ggtcagaacg tgcgtctggc ttcgcaactg
     6061 agcggttggg aactcaacgt gatgaccgtt gacgacctgc aagctaagca tcaggcggaa
     6121 gcgcacgcag cgatcgacac cttcaccaaa tatctcgaca tcgacgaaga cttcgcgact
     6181 gttctggtag aagaaggctt ctcgacgctg gaagaattgg cctatgtgcc gatgaaagag
     6241 ctgttggaaa tcgaaggcct tgatgagccg accgttgaag cactgcgcga gcgtgctaaa
     6301 aatgcactgg ccaccattgc acaggcccag gaagaaagcc tcggtgataa caaaccggct
     6361 gacgatctgc tgaaccttga aggggtagat cgtgatttgg cattcaaact ggccgcccgt
     6421 ggcgtttgta cgctggaaga tctcgccgaa cagggcattg atgatctggc tgatatcgaa
     6481 gggttgaccg acgaaaaagc cggagcactg attatggctg cccgtaatat ttgctggttc
     6541 ggtgacgaag cgactagtgg ttctggtcat caccatcacc atcactccgc gggtgagaat
     6601 ctttattttc agggcgcaca tatgagcaag ctttcgctca gcgagaagga gaaagccaac
     6661 aacttgccgg ttcgccctgg ccatggtacc atgggcgaga aggtgaagct ttgggccaac
     6721 tatttcaaaa tcaacatcaa atcaccagcc atttacaggt acaccatcaa agttgccgcc
     6781 accgaggaaa agctcggaaa ggaagctgag gtcgcatcca agaaagtgga ggtggtggtt
     6841 gggaaactgc tcaagcagat cgaagccaac gtgaaatccg tggcgattgc cagcgatttc
     6901 aaagtgcacc tggtgacgac caccaagctc aaagttcccg agaaccgcat ctttgaggtg
     6961 acgtggaccg agccgagttc caaccaaaac ctgcccagca agccccagac ttgggtggtc
     7021 aaggtggagg agagtgtcga aacctgcgat ttcggcaagg tgctgaacga gctcacgaca
     7081 cttgatccca agctcgacgg agactttccc aagtacaatg tggagctcga tgccctcaac
     7141 accattgtga ctcatcatgc ccgcgccgac gacaatgttg cggtggtggg aaggggaagg
     7201 ttttttgcca ttggtgatga cctcattgaa caagtgcggc cccatgactc ccctttggtc
     7261 atcttgcgag gatattttgc cagcgtccgt ccagctaccg gaagactttt actcaatacc
     7321 aacatcacgc atggtgtctt ccgtcctggg gtcaaacttg cacagctgtt tcaggaactt
     7381 ggacttgacg taatggacaa atgcaatgcc tggaacgaag taaccaaaaa tcagctcaac
     7441 gacaagatgc gcagagttca caaggtcctg gctaagggcc gtgtcgagtt gaatgcccca
     7501 ttccttattg atggaaagat tgtttataaa aaatgttacc gcacgctcaa tggcattgct
     7561 aaccgtggcg acgaaagggg gaagcaaaag gatggtaaag aagtccgata tccgcccttg
     7621 ttcgggattc cgggtgtcca ggttggcggc ccgacctctt gtcagttcta cttgcgtgcg
     7681 cgagagacaa aggatggcgc tgcccctcct ccgactcccg gcctgccgag caacgcgtac
     7741 atcacggtag cgaactatta taaacaacgg tacggaataa ccgccaatgc ttcgcttcct
     7801 ctggtcaacg ttggcaccaa ggaaaaggcg atttacgtct tggccgagtt ttgtacgctg
     7861 gtcaaaggcc gttccgtcaa ggctaagctg acagccaacg aggcggacaa catgattaag
     7921 tttgcttgca gagctccttc gctgaacgct cagtctatcg tgacgaaagg cagacagaca
     7981 cttggtcttg ataaaagcct gacgcttggc aagttcaagg tttcgatcga caaggagctg
     8041 atcaccgttg tcgggcgtga gctcaagcct ccgatgctta cgtacagcgg taacaagacg
     8101 gtagagccgc aggacggcgg gtggttgatg aagtttgtca aggtcgccag accttgccgc
     8161 aagattgaga agtggacata cttggaactg aagggttcca aggcaaacga aggggtgccg
     8221 caagctatga ccgcttttgc cgaattcttg aacagaacgg gcatcccgat taaccccagg
     8281 ttctcgccgg gcatgagcat gtcagttcca gggagcgaaa aagagttctt tgccaaagtg
     8341 aaggaactca tgagctcgca ccaatttgtg gtggttcttt tacccagaaa ggatgttgcg
     8401 atctacaata tggtgaagcg ggctgccgat atcacatttg gcgttcacac agtctgttgt
     8461 gtagccgaaa agttccttag cactaagggg cagctggggt attttgccaa cgtcggcctc
     8521 aaggtcaacc tcaagtttgg cggcaccaat cacaatatca agacgcccat tcctttgctc
     8581 gccaagggga agacgatggt ggtgggctat gatgtcaccc atccgaccaa tctagcggct
     8641 ggacaatcgc ctgcatcggc tcccagtatt gtcggcctgg tctcaaccat cgaccaacac
     8701 cttggacaat ggcctgcaat ggtttggaac aacccgcacg gccaggagtc catgacggaa
     8761 cagtttacgg acaagttcaa gacgcgtctg gaactatggc gcagcaatcc cgcaaacaac
     8821 cgcagtctcc ccgagaatat cctgattttc cgcgatggcg tctccgaggg acagttccag
     8881 atggtcatca aggacgagct atccctggtt cgcgccgcct gcaagctggt gtatccagct
     8941 ggcaagctac cgcgtattac gctgattgtc tctgtcaagc gccaccagac tcgcttcttc
     9001 ccaacggacc cgaagcatat tcacttcaag tccaagagcc ccaaggaggg tactgtggtt
     9061 gaccgcggcg tgaccaacgt ccgctattgg gacttctttt tgcaggcgca cgcgtcgctc
     9121 cagggcacgg cccgctcggc tcactacaca gttctggtgg atgagatttt cagggccgac
     9181 tatggaaaca aggcggccga cacgctggag cagctgacgc atgacatgtg ttatctcttt
     9241 ggacgagcca ccaaggctgt cagtatctgc ccgcctgcgt actatgccga cttggtgtgc
     9301 gaccgggcgc gtatccatca gaaggagctc tttgacgccc tcgatgaaaa cgatagcgtt
     9361 aagaccgatg atttcgcaag atggggtaac tccggggctg ttcatcccaa ccttaggaac
     9421 tccatgtact atatctaagc ggccgcactc gagcaccacc accaccacca ctgagatccg
     9481 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     9541 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
     9601 atatccggat
//
