LOCUS       sequence-290233-        8884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsEDC4-del974-1265.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsEDC4-del974-1265_V
FEATURES             Location/Qualifiers
     source          1..8884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1185..1211
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4588..4709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4599..4618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4653..4672
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4895..5350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4982..5001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5192..5213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5467..5824
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5467..5658
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5490..5510)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5675..5810
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5875..6669
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5929..5948)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6539..6558
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6733..6781
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(6822..6841)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6941..6963
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7001..7019)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7087..7191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7192..7260,7261..8052)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7410..7429)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8223..8811
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8712..8731
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg agaattcaat ggcttaccca tacgatgttc
     1201 cagattacgc tatggcctcc tgcgcgagca tcgacatcga ggacgccacg cagcacctgc
     1261 gggacatcct caagctggac cggcccgcgg gcggccccag tgcagagagc ccacggccat
     1321 ccagtgccta caatggggac ctcaatggac ttctggtccc agacccgctc tgctcaggtg
     1381 atagtacctc agcaaacaag actggtcttc ggaccatgcc acccattaac ctgcaagaga
     1441 agcaggtcat ctgtctctca ggagatgata gctccacctg cattgggatt ttggccaagg
     1501 aggtggagat tgtggctagc agtgactcta gcatttcaag caaggcccgg ggaagcaaca
     1561 aggtgaaaat tcagcctgtc gccaagtatg actgggaaca gaagtactac tatggcaacc
     1621 tgattgctgt gtctaactcc ttcttggcct atgccattcg ggctgccaac aatggctctg
     1681 ccatggtgcg ggtgatcagc gtcagcactt cggagcggac cttgctcaag ggcttcacag
     1741 gcagtgtggc tgatctggct ttcgcgcacc tcaactctcc acagctggcc tgcctggatg
     1801 aggcaggcaa cctgttcgtg tggcgcttgg ctctggttaa tggcaaaatt caagaagaga
     1861 tcttggtcca tattcggcag ccagagggca cgccactgaa ccactttcgc aggatcatct
     1921 ggtgcccctt catccctgag gagagcgaag actgctgtga ggagagcagc ccaacagtgg
     1981 ccctgctgca tgaagaccgg gctgaggtgt gggacctgga catgctccgc tccagccaca
     2041 gtacctggcc tgtggatgtt agccagacca agcagggctt cattgtggta aaaggtcata
     2101 gcacgtgcct cagtgaagga gccctctctc ctgatgggac tgtgctggct actgcgagcc
     2161 acgatggcta tgtcaagttc tggcagatct acattgaggg gcaagatgag ccaaggtgtc
     2221 tgcacgagtg gaaacctcat gatgggcggc ccctctcctg cctcctgttc tgtgacaacc
     2281 ataagaaaca agaccctgat gtccctttct ggaggttcct tattactggt gctgaccaga
     2341 accgagagtt aaagatgtgg tgtacagtat cctggacctg cctgcagact attcgcttct
     2401 ccccagatat cttcagctca gtgagtgtgc cccctagcct caaggtttgc ttggacctct
     2461 cagcagaata cctgattctc agcgatgtgc aacggaaggt cctctatgtg atggagctgc
     2521 tgcaaaacca ggaggagggc cacgcctgct tcagctccat ctcggagttc ctgctcaccc
     2581 accctgtgct gagctttggt atccaggttg tgagtcgctg ccggctacgg cacactgagg
     2641 tgctgcctgc cgaagaggaa aatgacagcc tgggtgctga tggtacccat ggagccggtg
     2701 ccatggagtc tgcggccggt gtgctcatca agctcttttg tgtgcatact aaggcactgc
     2761 aagatgtgca gatccgcttc cagccacagc tgaaccctga tgtggtggcc ccactgccca
     2821 cccacactgc ccacgaggac ttcacatttg gagagtctcg gcccgaactg ggctctgagg
     2881 gcctggggtc agccgctcac ggctcccagc ctgacctccg acgaatcgtg gagctgcctg
     2941 cacctgccga cttcctcagt ctgagcagtg agaccaagcc caagttgatg acacctgacg
     3001 ccttcatgac acctagcgcc tccttgcagc agatcactgc ctctcccagc agcagcagca
     3061 gcggtagcag cagcagcagc agcagtagca gcagctccct tacagctgtg tctgccatga
     3121 gcagcacctc agctgtggac ccctccttga ccaggccacc tgaggagctg accttgagcc
     3181 ccaagctgca gctggatggc agcctgacaa tgagcagcag tggcagcctt caggcaagcc
     3241 cgcgtggcct cctgcctggc ctgctcccag ccccagctga caaactgact cccaaggggc
     3301 cgggccaggt gcctactgcc acctctgcac tgtccctgga gctgcaggaa gtggagcccc
     3361 tggggctacc ccaagcctcc cctagccgca ctcgttcccc tgatgtcatc tcctcagctt
     3421 ccactgccct gtcccaggac atccctgaga ttgcatctga ggccctgtcc cgtggttttg
     3481 gctcctctgc accagagggc cttgagccag acagtatggc ttcagccgcc tcggcactgc
     3541 acctgctgtc cccacggccc cggccagggc ccgagctcgg cccccagctc gggcttgatg
     3601 gaggccctgg ggatggagat cggcataata ccccctccct cctggaggca gccttgaccc
     3661 aggaggcctc gactcctgac agtcaggttt ggcccacagc acctgacatt actcgtgaga
     3721 cctgcagcac cctggcagaa agccccagga atggccttca ggaaaagcac aagagcctgg
     3781 ccttccaccg accaccatat cacctgctgc agcaacgtga cagccaggat gccagtgctg
     3841 agcaaagtga ccatgatgat gaggtggcca gccttgcctc tgcttcagga ggctttggca
     3901 ccaaagttcc tgctccacgg ctgcctgcca aggactggaa gaccaaggga tcccctcgaa
     3961 cctcacccaa gctcaagagg aaaagcaaga aggatgatgg ggatgcagcc atgggatccc
     4021 ggctcacaga gcaccaggtg gcagagcccc ctgaggactg gccagcacta atttggcaac
     4081 agcagagaga gctggcagag ctgcggcaca gccaggaaga gctgctgcag gcccatatcc
     4141 tgcagctgct gcagcagggc cacctcaatc aggccttcca gcaggcgctg acagctgctg
     4201 acctgaacct ggtgctgtat gtgtgtgaaa ctgtggaccc agcccaggtt tttgggcagc
     4261 caccctgccc gctctcccag cctgtgctcc tttccctcat ccagcagctg gcatctgacc
     4321 ttggcactcg aactgacctc aagctcagct acctggaaga ggccgtgatg cacctggacc
     4381 acagtgaccc catcactcgg gaccacatgg gctccgttat ggcccaggtg cgccaaaagc
     4441 tttttcagtt cctgcaggct gagccacaca actcacttgg caaagcagct cggcgtctca
     4501 gcctcatgct gcatggcctc gtgaccccca gcctccctta ggcggccgct tccctttagt
     4561 gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     4621 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     4681 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     4741 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     4801 aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca ccgatcgccc
     4861 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     4921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5101 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5221 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5341 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     5401 tacgcggatc tgcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
     5461 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5641 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5701 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5761 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5821 caaaaagctt gattcttctg acacaacagt ctcgaactta aggctagagc caccatgatt
     5881 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     5941 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6001 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6061 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6121 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6181 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6241 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6301 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6361 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6421 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6481 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6541 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6601 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6661 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6721 cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     6781 gaatcgatag cgataaggat ccgcgtatgg tgcactctca gtacaatctg ctctgatgcc
     6841 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6901 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6961 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     7021 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     7081 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7141 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7201 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7261 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7321 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7381 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7441 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7501 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7561 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     7621 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     7681 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     7741 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     7801 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7861 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7921 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7981 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8041 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     8101 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     8161 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8221 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8281 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8341 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     8401 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8461 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8521 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8581 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8641 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8701 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     8761 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     8821 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag
     8881 atct
//
