LOCUS       sequence-290306-       13545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRN...
FEATURES             Location/Qualifiers
     source          1..13545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             10830..10871
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10881..10898
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10921..10938)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10971..11105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10995..11014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11049..11068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11727..12315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11807..11826)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12486..13277,13278..13346))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13109..13128
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13347..13451)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13519..13537
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttaattaaga tgaacgtaga gagccaactg aagacaccgc caacgcacat
     3361 tcgaaatctg gtcaagcacg tgaacaagcg aaattttaat gagagcagcg agcaaataaa
     3421 tcagttcgtt aaagagcacg gcctggaagc ggatcgcagc agcctgcgcc acctgttctc
     3481 cgtgatcaac ttcagcgatc tcgtgccatc agttacggtc cagctgcagg cgaagctcct
     3541 gggcatccag ttggagcgtc agcttcacag ttcatcgttt gtgtccaaca tctgctacgc
     3601 ttttgaccag ttcttcgcca gcaaccagaa gtccctgaag cctgttgcgg tagcggatct
     3661 cgttggccag gtcgcccgac tgaccggcat caacaaggtt tgtgagtgcg tctttgcctt
     3721 ggccttgacc cactcctcgt acacagaact caaacattct gccaggaaca acctaaaagg
     3781 tagcctcagc gaacttatag actcttactt gggtaataaa ggcaccggcc cagccgacag
     3841 cggtctccgg gagatctcct tcgatctact gcagtatttg ctgtgctgcc ttagcgagta
     3901 cgtacaaccg caggtggaag cacagttttt gatcaagctt cgtgaggagt ttccgcgcca
     3961 ggcggtaccg ctggttctag cgccgttcct ttacggctcg acgacggcga cgattgcggg
     4021 agcaggtgcc agcgaaacgg atgcggaggc cgaagcgacg actaatagca acagctccag
     4081 tttcgaagca gatgctttga acgaggtggg aatcgaggat atttatgacc atttaagcga
     4141 gataatattc accaaccagg gcaaaaataa tattatggac acatcttgga ttaatctaat
     4201 ccttgaaatc ggttatgaat ttacatcgag cgttgaagag tgtaagaatc atttgtgttc
     4261 ccgcgagcga gaacgcgctg agctccagtc caaagatgtc gccaagatcg tgggtcttat
     4321 gtgccgacgg cactcctccc tgctcgattg taatgtgaat ctaccgacgc cggcgaactt
     4381 ctggcctggt cagggacagg gcggtggcag caacagcagt ggctcttcgc agacacaaat
     4441 cactccgcag caacagaacc cgggcagcag taacaacaac gatggcagcg atggcaattc
     4501 gtccagtgat aagaaggaca agaaggagac gacggaggcc acgcaaactt ggaagccaga
     4561 tgtctttgtg caggcgctca aggaggtggt gcctcagctc aactggaagg acgtgtgcat
     4621 ggaacttgat catcccgagt tcgtgctgaa ggatcgcatc ggtttggagc ttttgctcac
     4681 catccttcgg ctggccactg gctctaacat atttccacat ccagaatgca tttaccgtca
     4741 ctgggcaaac acggaaggtc agctgtcgct tatcgcgaca atgctgaaga acccggatct
     4801 cttttccttc gccgactttg tatttagcca gccagcgttg gatgtgctta agacagctcc
     4861 ggatgcggac aataaggaga tctcgtcctg gaagtcgctt cacctggtgg aggtgctgct
     4921 ctccattgcg gacaagggat actatacgca ggtccatgag ctattcaagt tcccagcgca
     4981 gaactgtccc gatgtgctat ttttggcctt attgaacaca agtccgccta tgacgccact
     5041 gcgtcaggat ctgtttaacc aactgatacc aacgttcctt ggcaaccatc ccaactcgaa
     5101 cgtgatcttg gccagcgcct ggagctcgaa cagcttccaa cttcgttcca atatcatgaa
     5161 tgctatgtcc gagtggtatc tccgaggcaa tgagttcgat caggtgaaac tgtcgcgcat
     5221 cctggatctt gcccaggatt taaaagcgct atcagcgctg ctcaacgccc gttcattttt
     5281 attcattatc gacctggctt gccttgcctc tcggcgcgag tacctgaagc tggaaaagtg
     5341 gcttaccgat aaaattcgcg aacatggaga gccttttatg caggctataa tcaaggttct
     5401 gcaccgccgc tgcccgcagg ttatcaacgc caaagtgcca gaggatcagc tgcctcccaa
     5461 gcaggcgcag cttttgcccg agacagttac aaccatgatc aactgtctgc aaacgtgcat
     5521 caataactgc atgcagccgg agatggtcga agtcatcatg caaatgaccg cgaacgtggc
     5581 gattatggct aacatggcgc gtgcccaaca gcagcagcag ccaggattgg ttccgccgcc
     5641 tccaccaaca attctgcgcg gccatcgtgg tatggatctg cctggaggca tagtccctcc
     5701 gccaccgcag caaccgttct ccggaaacct caacgcgcag atgtttggac caggaatgga
     5761 tccgctaacg aatatgtcca acaacttagc cggcctcaac ctcagcggcc cgaacggagc
     5821 atttaatttt ggaaacatgc taacttctcc atcgcgtctg atgactccgg gagccaatcc
     5881 ctatccgctg aacctcatgc agatgccgca ggctccgcca ccacccaacg taggaaacct
     5941 aggccgcatg ctgccagggg gtccacaaca gcagactcca acgccgactc caacggcccc
     6001 caatccaaac aaccctgtga tggccgatct ccagatacct gtgtcaaagg aagttgaaga
     6061 cgaggtcaac tcatactttc agcgcatcta caaccaccag ccgaatccaa cgctgtccat
     6121 cgatgaagtg ctggacattt tgcaaagatt taaagagtca agtaaccggc gcgagcagga
     6181 agtctttttg tgcatgctgc gcaatctgtt cgaggaatat cgctttttct gtcaataccc
     6241 agagaaagag ctacagatta ctgcgcagct atttggtggc attattgatc gtaacctggt
     6301 gcccactttt gttgcactgg gcctttctct gcgctgtgtc ttggatgccc tccgcaagcc
     6361 cgatggatct aagctttact atttcggtgt gacggcactg gacagattca gaactcgatt
     6421 gcacacctac aacaaatact gcgagcatat ccgctccatt cctcacttct ctgacttccc
     6481 gccgcacctt attcagtatg tggaatacgg aatgcacggc caggagccgc cgccgcagaa
     6541 gttgattgga ctcagcaata cgattccttc ggcgatatct agcggaccag gaacggaacc
     6601 aatttacagg aatagctcca tgttaggtaa tatgcccgct gccaccccgg gatcgggacc
     6661 gaaatcaaac gctgctgtat cgcatgccac gagaatgaag tccatcgcca atgccaccaa
     6721 tatcgacaca cttttggtgg ccaaccagga agagaaagtg actgtgccac cagagcctgt
     6781 gcagggctca tctggcatac cgcagaagtg cgatgagata aaggagatta tgaccaagga
     6841 gtattggccg tggctagcgc aatatctggt cctcaaagcg gcatcaatgg agttcgccgc
     6901 ccacacacta tattacaact ttttggatgc ccttaaaaac ggcgagatca accgattcgt
     6961 aaccaaagag accctacgca acatcaaggt actcttgcgc tccgataagg gagttattaa
     7021 tttctccgat cgaagtctgt tgaagaacct cggacactgg ttgggcatga tgaccttagg
     7081 tcgcaatcgc cccatcctgc agttggatct ggatcttaaa tcccttttgg ctgaggctta
     7141 ccacaagggc cagcaggaac tgctttttgt ggttcctttc gtagccaaga tccttgagtc
     7201 atccgccaag tctcgcatct tccgatcgcc caatccatgg acaatgggta tcatgtacgt
     7261 gcttgctgag cttcaccagg agccagacct taagctgaac ctgaagtttg aaatcgaagt
     7321 actctgcaaa acacttaatc tggagttggc caagttgcga caagtgatct acctgaagga
     7381 tcccaatcga actcaccgga ttgaagagca gatgtcgcaa ccaaaaccga aacaacttga
     7441 gccagtggct tctgcgccag ctctaccgcg tgaacagcaa tccccggcac aacctccgcc
     7501 accaccacaa cagcagcaac caccgcagca gcaggtacct ccaccgccat cctctgcgga
     7561 cgtggacgcc caaaatgcgg ccgctatgat gatggcagcg ggcggagcta atagtactcc
     7621 tggatcggta tcttcgccca atctacccac cgattccagc caggtggcac taccgccacc
     7681 agagccgcgc tattcatacg tggacgtaaa cgtgagcaac ttccagctta ttggtcagca
     7741 gttggtactg ccacccaata ctccgttcct gcacgccaat cccggaatca aacacatggt
     7801 cgtcaacgcc gtggagcgca cgataaccga ctggttgcag cctattgtgg accgaagcat
     7861 ccgcattgcc tgtgccacca ccgagcagat catccgcaag gacttcgccc tggatgctga
     7921 cgagaaccga atgcgcactg ccgctcatca gatggtacga aaccttgccg ccggcatggc
     7981 catgattacc ggtaaggatg agatagcgcg tgctattagc cagaatctgc acaaggcctt
     8041 gctgtcgggt ctaaatggga tgcccagcat ggctgagatc caagctgctg ctatgcagtt
     8101 agccagcgaa aatgttgagc tagtttgcgc tttcattcag aaaacatctg ctgaaaaggc
     8161 agccgcagag atcgataggc gtctaagcac cgatttcgag accagaaaaa ttgctcgtga
     8221 agagggcaac cgctttgtgg acgcccagat cctcacctat caacaggagc gtcttccaga
     8281 agcagtgcgc atcaaggtgg gcgcggctcc agctacactt tatgctgtgt actcggagtt
     8341 cgctagaagc atacctggct tccagcagat gagcgatcgc gatattgccc tgtttgtgcc
     8401 caagccgacg gatttgtctc agccaaatgt gtttgctaat gatgatagca tggtatacgg
     8461 ggaactggct agcaaaatgg aggccttcat gaacacggca atcggagtgc cgacactcca
     8521 gatccaggct agcaagatgc acatgctact caacgccctg atagcgacgc gtcgactgcg
     8581 tgaccaagag tctgccttta atctgctgac ccgcgcggtc gagggtttga ccgaaggatt
     8641 ggtcaatatg catgaaaaca tggagcaaat gaagatgtac caaaacatcc atctccgtat
     8701 ccttggcttg ctgaacaaca gtttcggtgc tccaaacaca gagcgagcgg taacaaagtg
     8761 tttcttcgat atccgagagg aggtgcgata taacgtggag gcagctcgcg ctttgattac
     8821 gtcgcacttt gtcaatctga atcagtttga cggaatgcta cgcgactgca tggacaacgg
     8881 aaacaattat gtggctatat cgttcggtat cgcactgctg gagcgcctca tcatggacga
     8941 tcgagtgatt aatattgttt cggacaacga attcatggca actgttgagc tgctaggtag
     9001 gctgacccaa caccgtcacc gctaccccga gtgtatcgtc aacgccatcg acaccctgtg
     9061 gtccggcaat ttcaatagct cctccgatta ttcccccttt aatggaaacg accgctatct
     9121 gagcggcgct agccactata ttcatagcgg aatgcaccat gtccgctcgt gtgacaccga
     9181 cgaccccccc ggactccagg aaaaaaccga attcttgttg aaagactggg tggccttgta
     9241 tacccagcaa aatcaacaat cgacccgtga cgcccgtaat tttggcgcct tcgtgcaaaa
     9301 aatgaatacc tacggcattt tgaagaccga tgatctgatt acgcgcttct ttcgtcaagc
     9361 tacccatatc tgtaccgacg tcgtctatcg tatgtttgct gaacccagcc ttcccattaa
     9421 tcaagctaag aataaaattt ttcaatggat tgacgccttt gtgcacttga tcgccatgct
     9481 ggtccgccat tcgggagagg ccggcaaccc cacgacgaaa attaatttgc tcaataaagt
     9541 cttgggaatc gtcttgggca ccttgatcaa agatcacgaa atgcgcggag tgtcctttca
     9601 acaagtggga tatcaccgtt tttttatgat gttgtttatg gaattgtgta ccgctgacgt
     9661 catcctggaa agcttgatgc atagcatcgt cagcgctttt gcttacacat atcacttgtt
     9721 gaatcccagc gtggctccgg gattctgctt tgcttggttg gagctcattt cgcatcgcgt
     9781 cttcctgggc cgcatcctgg tccaaattcc cgggcaaaag ggctggccac tttacgctca
     9841 attgctgcag gaccttttta aatatcttgc gcccttctta cgcaacactg agctcggcaa
     9901 accagtccag cttttgtaca aggggacgct acgtgttctg ctcgttctgc ttcacgactt
     9961 cccggagttc ttgtgcgact atcattttgg gttctgtgac accatcccgc ccaattgcgt
    10021 ccagatgcgt aacatcatcc tgtcggcgtt tccgcgcaac atgcgcttgc ccgatccctt
    10081 cacgcccaac ctaaaagtgg acatgctgtc ggacagtagt aatgcgccta aggtgctcag
    10141 tagctacatt atgaacatcc agcccgcgaa ctttaagaag gatttggact cgtacctcaa
    10201 ggcccgggcg ccagttactt ttctatcgga attacgtggt cacttgcagg tgacaagcga
    10261 gccaggaact cgttacaaca tggcgctgat gaatgctctg gtcatgtatg tgggaactca
    10321 ggcgattgct ttgatcagaa acaaaaactt tgtgcccaac acctcgaaca tagcacacag
    10381 cgcccacatg gatatcttcc agaatcttgc tgttgacttg gatacggagg gtcgctatct
    10441 gttcttgaat gcgattgcga accagttgcg ctatcccaac agccacacgc actacttcag
    10501 ctgtgcggtg ctgcatctgt ttgccgaggc caactcggag gcaattcagg agcagatcac
    10561 tcgcgtactt ctggaacgat tgatcgtgaa ccgcccgcat ccttggggac tgttgatcac
    10621 ttttattgag ctgatcaaaa atcccatcta caagttctgg gaccatgact ttgtgcactg
    10681 tgcgccagag atcaccaagc tctttgaatc ggtggcccgt tcctgtctgg ccaagtcgaa
    10741 cgtcacccag cagttgaaca tgcccgtcgt cgatggcgag ggccaggagg tagccaccat
    10801 caactgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc
    10861 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
    10921 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca
    10981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
    11041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
    11101 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
    11161 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg
    11221 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
    11281 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
    11341 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
    11401 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
    11461 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
    11521 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
    11581 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
    11641 catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac
    11701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
    11941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    12061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    12121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    12181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12781 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    13021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    13081 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
    13141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    13501 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
