LOCUS       sequence-290322-       12948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 12948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: 
            pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
FEATURES             Location/Qualifiers
     source          1..12948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10233..10274
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10284..10301
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10324..10341)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10374..10508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10398..10417)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10452..10471
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11130..11718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11210..11229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11889..12680,12681..12749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12512..12531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12750..12854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12922..12940
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttaatta agatgaacgt agagagccaa ctgaagacac cgccaacgca
     2761 cattcgaaat ctggtcaagc acgtgaacaa gcgaaatttt aatgagagca gcgagcaaat
     2821 aaatcagttc gttaaagagc acggcctgga agcggatcgc agcagcctgc gccacctgtt
     2881 ctccgtgatc aacttcagcg atctcgtgcc atcagttacg gtccagctgc aggcgaagct
     2941 cctgggcatc cagttggagc gtcagcttca cagttcatcg tttgtgtcca acatctgcta
     3001 cgcttttgac cagttcttcg ccagcaacca gaagtccctg aagcctgttg cggtagcgga
     3061 tctcgttggc caggtcgccc gactgaccgg catcaacaag gtttgtgagt gcgtctttgc
     3121 cttggccttg acccactcct cgtacacaga actcaaacat tctgccagga acaacctaaa
     3181 aggtagcctc agcgaactta tagactctta cttgggtaat aaaggcaccg gcccagccga
     3241 cagcggtctc cgggagatct ccttcgatct actgcagtat ttgctgtgct gccttagcga
     3301 gtacgtacaa ccgcaggtgg aagcacagtt tttgatcaag cttcgtgagg agtttccgcg
     3361 ccaggcggta ccgctggttc tagcgccgtt cctttacggc tcgacgacgg cgacgattgc
     3421 gggagcaggt gccagcgaaa cggatgcgga ggccgaagcg acgactaata gcaacagctc
     3481 cagtttcgaa gcagatgctt tgaacgaggt gggaatcgag gatatttatg accatttaag
     3541 cgagataata ttcaccaacc agggcaaaaa taatattatg gacacatctt ggattaatct
     3601 aatccttgaa atcggttatg aatttacatc gagcgttgaa gagtgtaaga atcatttgtg
     3661 ttcccgcgag cgagaacgcg ctgagctcca gtccaaagat gtcgccaaga tcgtgggtct
     3721 tatgtgccga cggcactcct ccctgctcga ttgtaatgtg aatctaccga cgccggcgaa
     3781 cttctggcct ggtcagggac agggcggtgg cagcaacagc agtggctctt cgcagacaca
     3841 aatcactccg cagcaacaga acccgggcag cagtaacaac aacgatggca gcgatggcaa
     3901 ttcgtccagt gataagaagg acaagaagga gacgacggag gccacgcaaa cttggaagcc
     3961 agatgtcttt gtgcaggcgc tcaaggaggt ggtgcctcag ctcaactgga aggacgtgtg
     4021 catggaactt gatcatcccg agttcgtgct gaaggatcgc atcggtttgg agcttttgct
     4081 caccatcctt cggctggcca ctggctctaa catatttcca catccagaat gcatttaccg
     4141 tcactgggca aacacggaag gtcagctgtc gcttatcgcg acaatgctga agaacccgga
     4201 tctcttttcc ttcgccgact ttgtatttag ccagccagcg ttggatgtgc ttaagacagc
     4261 tccggatgcg gacaataagg agatctcgtc ctggaagtcg cttcacctgg tggaggtgct
     4321 gctctccatt gcggacaagg gatactatac gcaggtccat gagctattca agttcccagc
     4381 gcagaactgt cccgatgtgc tatttttggc cttattgaac acaagtccgc ctatgacgcc
     4441 actgcgtcag gatctgttta accaactgat accaacgttc cttggcaacc atcccaactc
     4501 gaacgtgatc ttggccagcg cctggagctc gaacagcttc caacttcgtt ccaatatcat
     4561 gaatgctatg tccgagtggt atctccgagg caatgagttc gatcaggtga aactgtcgcg
     4621 catcctggat cttgcccagg atttaaaagc gctatcagcg ctgctcaacg cccgttcatt
     4681 tttattcatt atcgacctgg cttgccttgc ctctcggcgc gagtacctga agctggaaaa
     4741 gtggcttacc gataaaattc gcgaacatgg agagcctttt atgcaggcta taatcaaggt
     4801 tctgcaccgc cgctgcccgc aggttatcaa cgccaaagtg ccagaggatc agctgcctcc
     4861 caagcaggcg cagcttttgc ccgagacagt tacaaccatg atcaactgtc tgcaaacgtg
     4921 catcaataac tgcatgcagc cggagatggt cgaagtcatc atgcaaatga ccgcgaacgt
     4981 ggcgattatg gctaacatgg cgcgtgccca acagcagcag cagccaggat tggttccgcc
     5041 gcctccacca acaattctgc gcggccatcg tggtatggat ctgcctggag gcatagtccc
     5101 tccgccaccg cagcaaccgt tctccggaaa cctcaacgcg cagatgtttg gaccaggaat
     5161 ggatccgcta acgaatatgt ccaacaactt agccggcctc aacctcagcg gcccgaacgg
     5221 agcatttaat tttggaaaca tgctaacttc tccatcgcgt ctgatgactc cgggagccaa
     5281 tccctatccg ctgaacctca tgcagatgcc gcaggctccg ccaccaccca acgtaggaaa
     5341 cctaggccgc atgctgccag ggggtccaca acagcagact ccaacgccga ctccaacggc
     5401 ccccaatcca aacaaccctg tgatggccga tctccagata cctgtgtcaa aggaagttga
     5461 agacgaggtc aactcatact ttcagcgcat ctacaaccac cagccgaatc caacgctgtc
     5521 catcgatgaa gtgctggaca ttttgcaaag atttaaagag tcaagtaacc ggcgcgagca
     5581 ggaagtcttt ttgtgcatgc tgcgcaatct gttcgaggaa tatcgctttt tctgtcaata
     5641 cccagagaaa gagctacaga ttactgcgca gctatttggt ggcattattg atcgtaacct
     5701 ggtgcccact tttgttgcac tgggcctttc tctgcgctgt gtcttggatg ccctccgcaa
     5761 gcccgatgga tctaagcttt actatttcgg tgtgacggca ctggacagat tcagaactcg
     5821 attgcacacc tacaacaaat actgcgagca tatccgctcc attcctcact tctctgactt
     5881 cccgccgcac cttattcagt atgtggaata cggaatgcac ggccaggagc cgccgccgca
     5941 gaagttgatt ggactcagca atacgattcc ttcggcgata tctagcggac caggaacgga
     6001 accaatttac aggaatagct ccatgttagg taatatgccc gctgccaccc cgggatcggg
     6061 accgaaatca aacgctgctg tatcgcatgc cacgagaatg aagtccatcg ccaatgccac
     6121 caatatcgac acacttttgg tggccaacca ggaagagaaa gtgactgtgc caccagagcc
     6181 tgtgcagggc tcatctggca taccgcagaa gtgcgatgag ataaaggaga ttatgaccaa
     6241 ggagtattgg ccgtggctag cgcaatatct ggtcctcaaa gcggcatcaa tggagttcgc
     6301 cgcccacaca ctatattaca actttttgga tgcccttaaa aacggcgaga tcaaccgatt
     6361 cgtaaccaaa gagaccctac gcaacatcaa ggtactcttg cgctccgata agggagttat
     6421 taatttctcc gatcgaagtc tgttgaagaa cctcggacac tggttgggca tgatgacctt
     6481 aggtcgcaat cgccccatcc tgcagttgga tctggatctt aaatcccttt tggctgaggc
     6541 ttaccacaag ggccagcagg aactgctttt tgtggttcct ttcgtagcca agatccttga
     6601 gtcatccgcc aagtctcgca tcttccgatc gcccaatcca tggacaatgg gtatcatgta
     6661 cgtgcttgct gagcttcacc aggagccaga ccttaagctg aacctgaagt ttgaaatcga
     6721 agtactctgc aaaacactta atctggagtt ggccaagttg cgacaagtga tctacctgaa
     6781 ggatcccaat cgaactcacc ggattgaaga gcagatgtcg caaccaaaac cgaaacaact
     6841 tgagccagtg gcttctgcgc cagctctacc gcgtgaacag caatccccgg cacaacctcc
     6901 gccaccacca caacagcagc aaccaccgca gcagcaggta cctccaccgc catcctctgc
     6961 ggacgtggac gcccaaaatg cggccgctat gatgatggca gcgggcggag ctaatagtac
     7021 tcctggatcg gtatcttcgc ccaatctacc caccgattcc agccaggtgg cactaccgcc
     7081 accagagccg cgctattcat acgtggacgt aaacgtgagc aacttccagc ttattggtca
     7141 gcagttggta ctgccaccca atactccgtt cctgcacgcc aatcccggaa tcaaacacat
     7201 ggtcgtcaac gccgtggagc gcacgataac cgactggttg cagcctattg tggaccgaag
     7261 catccgcatt gcctgtgcca ccaccgagca gatcatccgc aaggacttcg ccctggatgc
     7321 tgacgagaac cgaatgcgca ctgccgctca tcagatggta cgaaaccttg ccgccggcat
     7381 ggccatgatt accggtaagg atgagatagc gcgtgctatt agccagaatc tgcacaaggc
     7441 cttgctgtcg ggtctaaatg ggatgcccag catggctgag atccaagctg ctgctatgca
     7501 gttagccagc gaaaatgttg agctagtttg cgctttcatt cagaaaacat ctgctgaaaa
     7561 ggcagccgca gagatcgata ggcgtctaag caccgatttc gagaccagaa aaattgctcg
     7621 tgaagagggc aaccgctttg tggacgccca gatcctcacc tatcaacagg agcgtcttcc
     7681 agaagcagtg cgcatcaagg tgggcgcggc tccagctaca ctttatgctg tgtactcgga
     7741 gttcgctaga agcatacctg gcttccagca gatgagcgat cgcgatattg ccctgtttgt
     7801 gcccaagccg acggatttgt ctcagccaaa tgtgtttgct aatgatgata gcatggtata
     7861 cggggaactg gctagcaaaa tggaggcctt catgaacacg gcaatcggag tgccgacact
     7921 ccagatccag gctagcaaga tgcacatgct actcaacgcc ctgatagcga cgcgtcgact
     7981 gcgtgaccaa gagtctgcct ttaatctgct gacccgcgcg gtcgagggtt tgaccgaagg
     8041 attggtcaat atgcatgaaa acatggagca aatgaagatg taccaaaaca tccatctccg
     8101 tatccttggc ttgctgaaca acagtttcgg tgctccaaac acagagcgag cggtaacaaa
     8161 gtgtttcttc gatatccgag aggaggtgcg atataacgtg gaggcagctc gcgctttgat
     8221 tacgtcgcac tttgtcaatc tgaatcagtt tgacggaatg ctacgcgact gcatggacaa
     8281 cggaaacaat tatgtggcta tatcgttcgg tatcgcactg ctggagcgcc tcatcatgga
     8341 cgatcgagtg attaatattg tttcggacaa cgaattcatg gcaactgttg agctgctagg
     8401 taggctgacc caacaccgtc accgctaccc cgagtgtatc gtcaacgcca tcgacaccct
     8461 gtggtccggc aatttcaata gctcctccga ttattccccc tttaatggaa acgaccgcta
     8521 tctgagcggc gctagccact atattcatag cggaatgcac catgtccgct cgtgtgacac
     8581 cgacgacccc cccggactcc aggaaaaaac cgaattcttg ttgaaagact gggtggcctt
     8641 gtatacccag caaaatcaac aatcgacccg tgacgcccgt aattttggcg ccttcgtgca
     8701 aaaaatgaat acctacggca ttttgaagac cgatgatctg attacgcgct tctttcgtca
     8761 agctacccat atctgtaccg acgtcgtcta tcgtatgttt gctgaaccca gccttcccat
     8821 taatcaagct aagaataaaa tttttcaatg gattgacgcc tttgtgcact tgatcgccat
     8881 gctggtccgc cattcgggag aggccggcaa ccccacgacg aaaattaatt tgctcaataa
     8941 agtcttggga atcgtcttgg gcaccttgat caaagatcac gaaatgcgcg gagtgtcctt
     9001 tcaacaagtg ggatatcacc gtttttttat gatgttgttt atggaattgt gtaccgctga
     9061 cgtcatcctg gaaagcttga tgcatagcat cgtcagcgct tttgcttaca catatcactt
     9121 gttgaatccc agcgtggctc cgggattctg ctttgcttgg ttggagctca tttcgcatcg
     9181 cgtcttcctg ggccgcatcc tggtccaaat tcccgggcaa aagggctggc cactttacgc
     9241 tcaattgctg caggaccttt ttaaatatct tgcgcccttc ttacgcaaca ctgagctcgg
     9301 caaaccagtc cagcttttgt acaaggggac gctacgtgtt ctgctcgttc tgcttcacga
     9361 cttcccggag ttcttgtgcg actatcattt tgggttctgt gacaccatcc cgcccaattg
     9421 cgtccagatg cgtaacatca tcctgtcggc gtttccgcgc aacatgcgct tgcccgatcc
     9481 cttcacgccc aacctaaaag tggacatgct gtcggacagt agtaatgcgc ctaaggtgct
     9541 cagtagctac attatgaaca tccagcccgc gaactttaag aaggatttgg actcgtacct
     9601 caaggcccgg gcgccagtta cttttctatc ggaattacgt ggtcacttgc aggtgacaag
     9661 cgagccagga actcgttaca acatggcgct gatgaatgct ctggtcatgt atgtgggaac
     9721 tcaggcgatt gctttgatca gaaacaaaaa ctttgtgccc aacacctcga acatagcaca
     9781 cagcgcccac atggatatct tccagaatct tgctgttgac ttggatacgg agggtcgcta
     9841 tctgttcttg aatgcgattg cgaaccagtt gcgctatccc aacagccaca cgcactactt
     9901 cagctgtgcg gtgctgcatc tgtttgccga ggccaactcg gaggcaattc aggagcagat
     9961 cactcgcgta cttctggaac gattgatcgt gaaccgcccg catccttggg gactgttgat
    10021 cacttttatt gagctgatca aaaatcccat ctacaagttc tgggaccatg actttgtgca
    10081 ctgtgcgcca gagatcacca agctctttga atcggtggcc cgttcctgtc tggccaagtc
    10141 gaacgtcacc cagcagttga acatgcccgt cgtcgatggc gagggccagg aggtagccac
    10201 catcaactga tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
    10261 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
    10321 cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc gaggatccag
    10381 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
    10441 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
    10501 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
    10561 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagtcga
    10621 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
    10681 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
    10741 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
    10801 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc
    10861 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc
    10921 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc
    10981 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac
    11041 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca aaaggccagg
    11101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    11161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    11221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    11281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
    11341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
    11581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    12001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    12061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    12121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    12181 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
    12241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    12301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    12361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    12421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    12481 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
    12541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    12601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    12781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    12901 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgt
//
