LOCUS       sequence-290328-       11250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAr...
FEATURES             Location/Qualifiers
     source          1..11250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             665..730
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             755..781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8038..8159
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8075..8094)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8129..8148
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8166..8621)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8303..8324)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8515..8534
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8648..8752
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8754..9111
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8754..8945
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8777..8797)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8962..9097
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9146..9940
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9146..9940
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9200..9219)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9810..9829
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10128..10147)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10172..10219
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10548..11136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11037..11056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
      721 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
      781 tcctccatct cgagctatga atcttgactc gctctcgctg gccttgtctc aaatcagcta
      841 cctggtggac aatttaacca agaaaaatta ccgagccagc cagcaggaaa tacagcatat
      901 tgtgaatcgg cacggtcctg aggcagacag gcatttatta cgctgcctat tttcgcatgt
      961 ggatttcagt ggcgatggta aaagcagtgg caaagatttc catcagactc agtttctgat
     1021 tcaggagtgt gcgttgctga ttacaaagcc aaattttatc tcgacgctgt cctatgccat
     1081 tgataatcca ttgcactatc agaagagttt aaagcctgca ccccacttat ttgcccagct
     1141 gagtaaagtg ctcaaattaa gcaaagtaca agaggtaatt tttggccttg ccctgttgaa
     1201 ttcttccagc tcagatctta gaggtttcgc tgcccagttt atcaaacaga agcttccaga
     1261 tcttctgcgt tcttacattg acgcagacgt cagtggaaat caagaaggtg gcttccaaga
     1321 tatagcaata gaggtcctac acctcctcct ctcccatctc ctctttgggc agaagggagc
     1381 ctttggagtt ggacaagaac agatagacgc ttttcttaag acgctgcgca gagattttcc
     1441 ccaagaacgc tgtcccgtgg tgctcgcacc acttttatac cctgaaaaac gggacattct
     1501 aatggacagg atcctgcctg attccggagg ggtagctaaa accatgatgg agagctcttt
     1561 ggctgatttc atgcaagaag taggctatgg cttttgtgca agtattgaag aatgtcgcaa
     1621 tataatcgtg cagtttggtg ttcgggaggt cacagctgcc caggttgcaa gggttttggg
     1681 aatgatggct cgaactcatt caggattaac agatggcatt ccattacaga gtatttctgc
     1741 tccgggcagt gggatctgga gtgatgggaa agataaaagt gatggagcac aggcacacac
     1801 atggaatgta gaagtcttga ttgacgttct taaagaactg aatccaagtt tgaatttcaa
     1861 ggaagtaact tatgaactgg accatcctgg atttcaaatt cgtgacagta aaggacttca
     1921 taatgtggtt tatggcattc agaggggttt gggtatggaa gtgttcccag tagacctcat
     1981 atatagacct tggaaacatg ctgaaggcca gctctccttc atccagcact cactaatcaa
     2041 tccagagatc ttctgttttg ctgactatcc ctgtcatact gttgccactg atattctgaa
     2101 agcaccacca gaggatgaca atcgagaaat tgccacatgg aagagcttgg atttgattga
     2161 atctctgctg aggcttgcag aggttgggca gtatgagcaa gtcaaacagc tcttcagctt
     2221 ccctatcaaa cactgtccag acatgctggt attggcctta ctacaaatta acacctcttg
     2281 gcataccttg cgccatgaac ttatctccac tctgatgcca attttccttg gaaaccatcc
     2341 taactcagct attattttgc actatgcatg gcatgggcag ggacagtctc cctcaattcg
     2401 ccaacttatc atgcatgcaa tggcagaatg gtacatgaga ggggagcagt atgatcaggc
     2461 caaattgtct cgaatacttg atgtggccca ggacttgaag gccttgtcaa tgctgctaaa
     2521 tggtactcca tttgcctttg ttattgacct tgctgcactt gcttcacgtc gtgaatacct
     2581 caaacttgat aagtggctca cagataaaat tcgagagcat ggggagcctt ttatccaggc
     2641 gtgtatgact tttttaaaga gacggtgtcc ttctattttg ggcggacttg ccccagaaaa
     2701 agaccagccc aaaagtgctc aacttcctcc agaaactttg gcgacaatgt tggcctgtct
     2761 gcaagcttgt gcagggagtg tttctcagga gctatcagaa actatcctca ccatggtagc
     2821 caattgcagt aatgttatga ataaggccag acaaccacca cctggagtta tgccaaaagg
     2881 acgtcctcct agtgctagca gcttagatgc catttctcct gttcagattg accctcttgc
     2941 tggaatgaca tctcttagta taggtggttc agctgcccct cacacccaga gtatgcaggg
     3001 ttttcctcca aatttgggtt ctgcattcag tacccctcag tcaccagcaa aagcatttcc
     3061 acccctttca acccccaatc agaccactgc attcagtggt attggaggac tttcatcaca
     3121 gcttccagta ggtggtcttg gcacaggcag cctgactggt ataggaactg gtgctcttgg
     3181 actccctgca gtgaataacg acccttttgt acagaggaaa ctgggcacct ctggactgaa
     3241 tcagcctaca ttccagcaga gtaagatgaa accttcggac ttgtctcagg tgtggccaga
     3301 ggcaaaccag cactttagta aagagataga tgatgaagca aacagctatt tccagcgaat
     3361 atataatcat ccaccacatc caaccatgtc tgttgatgag gtattagaaa tgctgcagag
     3421 atttaaagac tctactataa agagggaacg agaagtattt aactgtatgc taaggaactt
     3481 gtttgaagaa tatcgttttt ttccccagta tcctgataaa gagttacata taacagcctg
     3541 cctatttggt ggtataattg agaaaggact ggtcacttac atggcactag gtctggctct
     3601 acgatatgtt cttgaagcct tacgcaagcc ttttggatcc aaaatgtatt atttcgggat
     3661 tgctgcacta gatagattta aaaacagatt gaaggactat ccccagtatt gtcagcattt
     3721 ggcttctatc agtcacttta tgcaatttcc acatcattta caggagtata ttgagtatgg
     3781 acagcagtct agagatcctc ctgtgaaaat gcaaggctct atcacaaccc ctggaagtat
     3841 tgcactggct caggcccagg ctcaggccca ggttccagca aaagctcctc ttgctggtca
     3901 agttagcact atggtaacca cctcaacaac taccactgtt gctaaaacgg ttacggtcac
     3961 caggccaact ggagtcagct ttaagaaaga tgtgccacct tctattaata ctacaaatat
     4021 agatacgttg cttgtggcca cagatcaaac tgagagaatt gtggagcccc cagaaaatat
     4081 ccagggtagt agtggtatga cacaaaaggt tgaagagcta aaggaaacgg tgaaagaaga
     4141 atttatgcct tgggtttcac agtatctggt tatgaaggca gtcagtattg agccaaactt
     4201 tcatagcctg tattcaaact tccttgacac gctgaagaat cctgaattta acaagatggt
     4261 tctgaatgag acctacagaa acattaaagt gctcctgacc tctgataaag ctgcagccaa
     4321 tttctcagat cgttctttgc tgaagaactt gggacattgg ctaggaatga tcacattagc
     4381 taaaaacaaa cccatcttac acactgactt ggatgtgaaa tcattgctgc tagaggctta
     4441 tgttaaagga caacaagaat tgctctatgt agtgcccttt gttgccaaag tcttagaatc
     4501 tagcattcgt agtgtggttt ttaggccacc aaacccttgg acaatggcaa ttatgaatgt
     4561 attagctgag ctacatcagg agcatgactt aaagttaaac ttgaagtttg aaatcgaggt
     4621 tctctgcaag aaccttgcat tagacatcaa tgagctaaaa cctggaaacc tcctaaagga
     4681 taaagatcgc ctgaagaatt tagatgagca actctctgct ccaaagaaag atgtcaagca
     4741 gccagaagaa ctccctccca tcacaaccac aacaacttct actacaccag ctaccaacac
     4801 cacttgtaca gccacggttc caccacagcc acagtacagc taccacgaca tcaatgtcta
     4861 ttcccttgcg ggcttggcac cacacattac tctaaatcca acaattccct tgtttcaggc
     4921 ccatccacag ttgaagcagt gtgtgcgtca ggcaattgaa cgggctgtcc aggagctggt
     4981 ccatcctgtg gtggatcgat caattaagat tgccatgact acttgtgagc aaatagtcag
     5041 gaaggatttt gccctggatt cggaggaatc tcgaatgcga atagcagctc atcacatgat
     5101 gcgtaacttg acagctggaa tggctatgat tacatgcagg gaacctttgc tcatgagcat
     5161 atctaccaac ttaaaaaaca gttttgcctc agcccttcgt actgcttccc cacaacaaag
     5221 agaaatgatg gatcaggcag ctgctcaatt agctcaggac aattgtgagt tggcttgctg
     5281 ttttattcag aagactgcag tagaaaaagc aggccctgag atggacaaga gattagcaac
     5341 tgaatttgag ctgagaaaac atgctaggca agaaggacgc agatactgtg atcctgttgt
     5401 tttaacatat caagctgaac ggatgccaga gcaaatcagg ctgaaagttg gtggtgtgga
     5461 cccaaagcag ttggctgttt acgaagagtt tgcacgcaat gttcctggct tcttacctac
     5521 aaatgactta agtcagccca cgggattttt agcccagccc atgaagcaag cttgggcaac
     5581 agatgatgta gctcagattt atgataagtg tattacagaa ctggagcaac atctacatgc
     5641 catcccacca actttggcca tgaaccctca agctcaggct cttcgaagtc tcttggaggt
     5701 tgtagtttta tctcgaaact ctcgggatgc catagctgct cttggattgc tccaaaaggc
     5761 tgtagagggc ttactagatg ccacaagtgg tgctgatgct gaccttctgc tgcgctacag
     5821 ggaatgccac ctcttggtcc taaaagctct gcaggatggc cgggcatatg ggtctccatg
     5881 gtgcaacaaa cagatcacaa ggtgcctaat tgaatgtcga gatgaatata aatataatgt
     5941 ggaggctgtg gagctgctaa ttcgcaatca tttggttaat atgcagcagt atgatcttca
     6001 cctagcgcag tcaatggaga atggcttaaa ctacatggct gtggcatttg ctatgcagtt
     6061 agtaaaaatc ctgctggtgg atgaaaggag tgttgctcat gttactgagg cagatctgtt
     6121 ccacaccatt gaaaccctca tgaggattaa tgctcattcc agaggcaatg ctccagaagg
     6181 attgccccag ctgatggaag tagtgcgatc caactatgaa gcaatgattg atcgtgctca
     6241 tggaggccca aactttatga tgcattctgg gatctctcaa gcctcagagt atgatgaccc
     6301 tccaggcctg agggagaagg cagagtatct tctgagggaa tgggtgaatc tctaccattc
     6361 agcagcagct ggccgcgaca gtaccaaagc tttctctgca tttgttggac agatgcacca
     6421 gcaaggaata ctgaagaccg atgatctcat aacaaggttc tttcgtctgt gtactgaaat
     6481 gtgtgttgaa atcagttacc gtgctcaggc tgagcagcag cacaatcctg ctgccaatcc
     6541 caccatgatc cgagccaagt gctatcacaa cctggatgcc tttgttcgac tcattgcact
     6601 gctcgtgaaa cactcagggg aggccaccaa cactgtcaca aagattaatc tgctgaacaa
     6661 ggtccttggt atagtagtgg gagttctcct tcaggatcat gatgttcgtc agagtgaatt
     6721 tcagcaactt ccctaccatc gaatttttat catgcttctc ttggaactca atgcacctga
     6781 gcatgtgttg gaaaccatta atttccagac acttacagct ttctgcaata cattccacat
     6841 cttgaggcct accaaagctc ctggctttgt atatgcctgg cttgaactga tttcccatcg
     6901 gatatttatt gcaagaatgc tggcacatac gccacagcag aaggggtggc ctatgtatgc
     6961 acagctactg attgatttat tcaaatattt agcgcctttc cttagaaatg tggaactcac
     7021 caaacctatg caaatcctct acaagggcac tttaagagtg ctgctggttc ttttgcatga
     7081 tttcccagag ttcctttgtg attaccatta tgggttctgt gatgtgatcc cacctaattg
     7141 tatccagtta agaaatttga tcctgagtgc ctttccaaga aacatgaggc tccccgaccc
     7201 attcactcct aatctaaagg tggacatgtt gagtgaaatt aacattgctc cccggattct
     7261 caccaatttc actggagtaa tgccacctca gttcaaaaag gatttggatt cctatcttaa
     7321 aactcgatca ccagtcactt tcctgtctga tctgcgcagc aacctacagg tatccaatga
     7381 acctgggaat cgctacaacc tccagctcat caatgcactg gtgctctatg tcgggactca
     7441 ggccattgcg cacatccaca acaagggcag cacaccttca atgagcacca tcactcactc
     7501 agcacacatg gatatcttcc agaatttggc tgtggacttg gacactgagg gtcgctatct
     7561 ctttttgaat gcaattgcaa atcagctccg gtacccaaat agccacactc actacttcag
     7621 ttgcaccatg ctgtaccttt ttgcagaggc caatacggaa gccatccaag aacagatcac
     7681 aagagttctc ttggaacggt tgattgtaaa taggccacat ccttggggtc ttcttattac
     7741 cttcattgag ctgattaaaa acccagcgtt taagttctgg aaccatgaat ttgtacactg
     7801 tgccccagaa atcgaaaagt tattccagtc ggtcgcacag tgctgcatgg gacagaagca
     7861 ggcccagcaa gtaatggaag ggacaggtgc cagttagccg cgggcccggg atccaccgga
     7921 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     7981 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8041 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8101 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     8161 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     8221 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     8281 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     8341 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     8401 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     8461 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     8521 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     8581 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     8641 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8701 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     8761 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     8821 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     8881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     8941 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9001 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9061 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     9121 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     9181 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     9241 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     9301 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     9361 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     9421 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     9481 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     9541 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     9601 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     9661 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     9721 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     9781 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     9841 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     9901 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     9961 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
    10021 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    10081 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
    10141 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
    10201 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
    10261 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
    10321 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
    10381 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    10441 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10501 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10561 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10621 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10681 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10741 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10801 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10861 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10921 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10981 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11041 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11101 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11161 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11221 tctgtggata accgtattac cgccatgcat
//
