LOCUS       sequence-290334-        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of 
            HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: 
            pT7-EGFP-C1-HsNot1iso1_1-1605-del1097-1110-RNFIKGRQtoAAADSYAA-shRNAr
            es_W
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6331..6452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6368..6387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6422..6441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6459..6914)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6596..6617)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6808..6827
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6941..7045
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7047..7404
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7047..7238
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7070..7090)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7255..7390
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7439..8233
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7439..8233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7493..7512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8103..8122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8421..8440)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8465..8512
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8841..9429
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9330..9349
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcatcc agcactcact
     2641 aatcaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagagtaa gatgaaacct tcggacttgt ctcaggtgtg
     3901 gccagaggca aaccagcact ttagtaaaga gatagatgat gaagcaaaca gctatttcca
     3961 gcgaatatat aatcatccac cacatccaac catgtctgtt gatgaggtat tagaaatgct
     4021 gcagagattt aaagactcta ctataaagag ggaacgagaa gtatttaact gtatgctaag
     4081 gaacttgttt gaagaatatc gtttttttcc ccagtatcct gataaagagt tacatataac
     4141 agcctgccta tttggtggta taattgagaa aggactggtc acttacatgg cactaggtct
     4201 ggctctacga tatgttcttg aagccttacg caagcctttt ggatccaaaa tgtattattt
     4261 cgggattgct gcactagata gatttaaaaa cagattgaag gactatcccc agtattgtca
     4321 gcatttggct tctatcagtc actttatgca atttccacat catttacagg agtatattga
     4381 gtatggacag cagtctagag atcctcctgt gaaaatgcaa ggctctatca caacccctgg
     4441 aagtattgca ctggctcagg cccaggctca ggcccaggtt ccagcaaaag ctcctcttgc
     4501 tggtcaagtt agcactatgg taaccacctc aacaactacc actgttgcta aaacggttac
     4561 ggtcaccagg ccaactggag tcagctttaa gaaagatgtg ccaccttcta ttaatactac
     4621 aaatatagat acgttgcttg tggccacaga tcaaactgag agaattgtgg agcccccaga
     4681 aaatatccag ggtagtagtg gtatgacaca aaaggttgaa gagctaaagg aaacggtgaa
     4741 agaagaattt atgccttggg tttcacagta tctggttatg aaggcagtca gtattgagcc
     4801 agccgctcat agcctgtatt caaacttcct tgacacgctg aagaatcctg aatttaacaa
     4861 gatggttctg aatgagacct acagaaacat taaagtgctc ctgacctctg ataaagctgc
     4921 agccaatttc tcagatcgtt ctttgctgaa gaacttggga cattggctag gaatgatcac
     4981 attagctaaa aacaaaccca tcttacacac tgacttggat gtgaaatcat tgctgctaga
     5041 ggcttatgtt aaaggacaac aagaattgct ctatgtagtg ccctttgttg ccaaagtctt
     5101 agaatctagc attcgtagtg tggtttttag gccaccaaac ccttggacaa tggcaattat
     5161 gaatgtatta gctgagctac atcaggagca tgacttaaag ttaaacttga agtttgaaat
     5221 cgaggttctc tgcaagaacc ttgcattaga catcaatgag ctaaaacctg gaaacctcct
     5281 aaaggataaa gatcgcctga agaatttaga tgagcaactc tctgctccaa agaaagatgt
     5341 caagcagcca gaagaactcc ctcccatcac aaccacaaca acttctacta caccagctac
     5401 caacaccact tgtacagcca cggttccacc acagccacag tacagctacc acgacatcaa
     5461 tgtctattcc cttgcgggct tggcaccaca cattactcta aatccaacaa ttcccttgtt
     5521 tcaggcccat ccacagttga agcagtgtgt gcgtcaggca attgaacggg ctgtccagga
     5581 gctggtccat cctgtggtgg atcgatcaat taagattgcc atgactactt gtgagcaaga
     5641 tgtcaggtcg gattttgccc tggattcgga ggaatctcga atgcgaatag cagctcatca
     5701 catgatgcgt aacttgacag cttacatggc tatgattaca tgcgctgaac ctttgctcat
     5761 gagcatatct accaacttaa aaaacagttt tgcctcagcc cttcgtactg cttccccaca
     5821 acaaagagaa atgatggatc aggcagctgc tcaattagct caggacaatt gtgagttggc
     5881 ttgctgtttt attcagaaga ctgcagtaga aaaagcaggc cctgagatgg acaagagatt
     5941 agcaactgaa tttgagctga gaaaacatgc taggcaagaa ggacgcagat actgtgatcc
     6001 tgttgtttta acatatgctg ctgaacggat gccagagcaa atcaggctga aagttggtgg
     6061 tgtggaccca aagcagttgg ctgtttacga agagtttgca cgcaatgttc ctggcttctt
     6121 acctacaaat gacttaagtc agcccacggg atttttagcc cagcccatga agcaagcttg
     6181 ggcaacatag ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat
     6241 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     6301 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     6361 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     6421 tgtggtttgt ccaaactcat caatgtatct taacgcgtaa attgtaagcg ttaatatttt
     6481 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     6541 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     6601 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     6661 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     6721 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     6781 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     6841 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     6901 gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6961 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7021 tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt
     7081 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7141 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7201 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7261 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7321 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7381 tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat
     7441 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     7501 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     7561 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     7621 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     7681 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     7741 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     7801 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     7861 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     7921 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg
     7981 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8041 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8101 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8161 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8221 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     8281 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8341 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8401 caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct
     8461 atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc
     8521 ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa
     8581 tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg
     8641 ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt
     8701 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8761 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8821 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8881 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8941 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     9001 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9061 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9121 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9181 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9241 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9301 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9361 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9421 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9481 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
     9541 cat
//
