LOCUS       sequence-290335-        9573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 9573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: 
            pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..9573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6361..6482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6398..6417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6452..6471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6489..6944)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6626..6647)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6838..6857
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6971..7075
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7077..7434
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7077..7268
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7100..7120)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7285..7420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7469..8263
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7469..8263
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7523..7542)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8133..8152
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8451..8470)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8495..8542
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8871..9459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9360..9379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcatcc agcactcact
     2641 aatcaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagagtaa gatgaaacct tcggacttgt ctcaggtgtg
     3901 gccagaggca aaccagcact ttagtaaaga gatagatgat gaagcaaaca gctatttcca
     3961 gcgaatatat aatcatccac cacatccaac catgtctgtt gatgaggtat tagaaatgct
     4021 gcagagattt aaagactcta ctataaagag ggaacgagaa gtatttaact gtatgctaag
     4081 gaacttgttt gaagaatatc gtttttttcc ccagtatcct gataaagagt tacatataac
     4141 agcctgccta tttggtggta taattgagaa aggactggtc acttacatgg cactaggtct
     4201 ggctctacga tatgttcttg aagccttacg caagcctttt ggatccaaaa tgtattattt
     4261 cgggattgct gcactagata gatttaaaaa cagattgaag gactatcccc agtattgtca
     4321 gcatttggct tctatcagtc actttatgca atttccacat catttacagg agtatattga
     4381 gtatggacag cagtctagag atcctcctgt gaaaatgcaa ggctctatca caacccctgg
     4441 aagtattgca ctggctcagg cccaggctca ggcccaggtt ccagcaaaag ctcctcttgc
     4501 tggtcaagtt agcactatgg taaccacctc aacaactacc actgttgcta aaacggttac
     4561 ggtcaccagg ccaactggag tcagctttaa gaaagatgtg ccaccttcta ttaatactac
     4621 aaatatagat acgttgcttg tggccacaga tcaaactgag agaattgtgg agcccccaga
     4681 aaatatccag gagaaaattg cttttatttt caataatctc tcacagtcaa atatgacaca
     4741 aaaggttgaa gagctaaagg aaacggtgaa agaagaattt atgccttggg tttcacagta
     4801 tctggttatg aagagagtca gtattgagcc aaactttcat agcctgtatt caaacttcct
     4861 tgacacgctg aagaatcctg aatttaacaa gatggttctg aatgagacct acagaaacat
     4921 taaagtgctc ctgacctctg ataaagctgc agccaatttc tcagatcgtt ctttgctgaa
     4981 gaacttggga cattggctag gaatgatcac attagctaaa aacaaaccca tcttacacac
     5041 tgacttggat gtgaaatcat tgctgctaga ggcttatgtt aaaggacaac aagaattgct
     5101 ctatgtagtg ccctttgttg ccaaagtctt agaatctagc attcgtagtg tggtttttag
     5161 gccaccaaac ccttggacaa tggcaattat gaatgtatta gctgagctac atcaggagca
     5221 tgacttaaag ttaaacttga agtttgaaat cgaggttctc tgcaagaacc ttgcattaga
     5281 catcaatgag ctaaaacctg gaaacctcct aaaggataaa gatcgcctga agaatttaga
     5341 tgagcaactc tctgctccaa agaaagatgt caagcagcca gaagaactcc ctcccatcac
     5401 aaccacaaca acttctacta caccagctac caacaccact tgtacagcca cggttccacc
     5461 acagccacag tacagctacc acgacatcaa tgtctattcc cttgcgggct tggcaccaca
     5521 cattactcta aatccaacaa ttcccttgtt tcaggcccat ccacagttga agcagtgtgt
     5581 gcgtcaggca attgaacggg ctgtccagga gctggtccat cctgtggtgg atcgatcaat
     5641 taagattgcc atgactactt gtgagcaaga tgtcaggtcg gattttgccc tggattcgga
     5701 ggaatctcga atgcgaatag cagctcatca catgatgcgt aacttgacag cttacatggc
     5761 tatgattaca tgcgctgaac ctttgctcat gagcatatct accaacttaa aaaacagttt
     5821 tgcctcagcc cttcgtactg cttccccaca acaaagagaa atgatggatc aggcagctgc
     5881 tcaattagct caggacaatt gtgagttggc ttgctgtttt attcagaaga ctgcagtaga
     5941 aaaagcaggc cctgagatgg acaagagatt agcaactgaa tttgagctga gaaaacatgc
     6001 taggcaagaa ggacgcagat actgtgatcc tgttgtttta acatatgctg ctgaacggat
     6061 gccagagcaa atcaggctga aagttggtgg tgtggaccca aagcagttgg ctgtttacga
     6121 agagtttgca cgcaatgttc ctggcttctt acctacaaat gacttaagtc agcccacggg
     6181 atttttagcc cagcccatga agcaagcttg ggcaacatag ccgcgggccc gggatccacc
     6241 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6301 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6361 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6421 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6481 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6541 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6601 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6661 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6721 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6781 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6841 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6901 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     6961 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7021 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     7081 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7141 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7201 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7261 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7321 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7381 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     7441 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     7501 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     7561 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     7621 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     7681 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     7741 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     7801 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     7861 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7921 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7981 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     8041 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     8101 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     8161 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     8221 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     8281 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     8341 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8401 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     8461 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     8521 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     8581 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     8641 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     8701 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     8761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9001 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9541 gattctgtgg ataaccgtat taccgccatg cat
//
