LOCUS       sequence-290345-       11856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-del1097-1110-NF1144AA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11856)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 11856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: 
            pT7-EGFP-C1-HsNot1iso1-del1097-1110-NF1144AA-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..11856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8644..8765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8681..8700)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8735..8754
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8772..9227)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8909..8930)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9121..9140
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9254..9358
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9360..9717
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9360..9551
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9383..9403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9568..9703
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9752..10546
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9752..10546
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9806..9825)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10416..10435
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10734..10753)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10778..10825
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11154..11742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11643..11662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcatcc agcactcact
     2641 aatcaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagagtaa gatgaaacct tcggacttgt ctcaggtgtg
     3901 gccagaggca aaccagcact ttagtaaaga gatagatgat gaagcaaaca gctatttcca
     3961 gcgaatatat aatcatccac cacatccaac catgtctgtt gatgaggtat tagaaatgct
     4021 gcagagattt aaagactcta ctataaagag ggaacgagaa gtatttaact gtatgctaag
     4081 gaacttgttt gaagaatatc gtttttttcc ccagtatcct gataaagagt tacatataac
     4141 agcctgccta tttggtggta taattgagaa aggactggtc acttacatgg cactaggtct
     4201 ggctctacga tatgttcttg aagccttacg caagcctttt ggatccaaaa tgtattattt
     4261 cgggattgct gcactagata gatttaaaaa cagattgaag gactatcccc agtattgtca
     4321 gcatttggct tctatcagtc actttatgca atttccacat catttacagg agtatattga
     4381 gtatggacag cagtctagag atcctcctgt gaaaatgcaa ggctctatca caacccctgg
     4441 aagtattgca ctggctcagg cccaggctca ggcccaggtt ccagcaaaag ctcctcttgc
     4501 tggtcaagtt agcactatgg taaccacctc aacaactacc actgttgcta aaacggttac
     4561 ggtcaccagg ccaactggag tcagctttaa gaaagatgtg ccaccttcta ttaatactac
     4621 aaatatagat acgttgcttg tggccacaga tcaaactgag agaattgtgg agcccccaga
     4681 aaatatccag ggtagtagtg gtatgacaca aaaggttgaa gagctaaagg aaacggtgaa
     4741 agaagaattt atgccttggg tttcacagta tctggttatg aagagagtca gtattgagcc
     4801 agccgctcat agcctgtatt caaacttcct tgacacgctg aagaatcctg aatttaacaa
     4861 gatggttctg aatgagacct acagaaacat taaagtgctc ctgacctctg ataaagctgc
     4921 agccaatttc tcagatcgtt ctttgctgaa gaacttggga cattggctag gaatgatcac
     4981 attagctaaa aacaaaccca tcttacacac tgacttggat gtgaaatcat tgctgctaga
     5041 ggcttatgtt aaaggacaac aagaattgct ctatgtagtg ccctttgttg ccaaagtctt
     5101 agaatctagc attcgtagtg tggtttttag gccaccaaac ccttggacaa tggcaattat
     5161 gaatgtatta gctgagctac atcaggagca tgacttaaag ttaaacttga agtttgaaat
     5221 cgaggttctc tgcaagaacc ttgcattaga catcaatgag ctaaaacctg gaaacctcct
     5281 aaaggataaa gatcgcctga agaatttaga tgagcaactc tctgctccaa agaaagatgt
     5341 caagcagcca gaagaactcc ctcccatcac aaccacaaca acttctacta caccagctac
     5401 caacaccact tgtacagcca cggttccacc acagccacag tacagctacc acgacatcaa
     5461 tgtctattcc cttgcgggct tggcaccaca cattactcta aatccaacaa ttcccttgtt
     5521 tcaggcccat ccacagttga agcagtgtgt gcgtcaggca attgaacggg ctgtccagga
     5581 gctggtccat cctgtggtgg atcgatcaat taagattgcc atgactactt gtgagcaaat
     5641 agtcaggaag gattttgccc tggattcgga ggaatctcga atgcgaatag cagctcatca
     5701 catgatgcgt aacttgacag ctggaatggc tatgattaca tgcagggaac ctttgctcat
     5761 gagcatatct accaacttaa aaaacagttt tgcctcagcc cttcgtactg cttccccaca
     5821 acaaagagaa atgatggatc aggcagctgc tcaattagct caggacaatt gtgagttggc
     5881 ttgctgtttt attcagaaga ctgcagtaga aaaagcaggc cctgagatgg acaagagatt
     5941 agcaactgaa tttgagctga gaaaacatgc taggcaagaa ggacgcagat actgtgatcc
     6001 tgttgtttta acatatcaag ctgaacggat gccagagcaa atcaggctga aagttggtgg
     6061 tgtggaccca aagcagttgg ctgtttacga agagtttgca cgcaatgttc ctggcttctt
     6121 acctacaaat gacttaagtc agcccacggg atttttagcc cagcccatga agcaagcttg
     6181 ggcaacagat gatgtagctc agatttatga taagtgtatt acagaactgg agcaacatct
     6241 acatgccatc ccaccaactt tggccatgaa ccctcaagct caggctcttc gaagtctctt
     6301 ggaggttgta gttttatctc gaaactctcg ggatgccata gctgctcttg gattgctcca
     6361 aaaggctgta gagggcttac tagatgccac aagtggtgct gatgctgacc ttctgctgcg
     6421 ctacagggaa tgccacctct tggtcctaaa agctctgcag gatggccggg catatgggtc
     6481 tccatggtgc aacaaacaga tcacaaggtg cctaattgaa tgtcgagatg aatataaata
     6541 taatgtggag gctgtggagc tgctaattcg caatcatttg gttaatatgc agcagtatga
     6601 tcttcaccta gcgcagtcaa tggagaatgg cttaaactac atggctgtgg catttgctat
     6661 gcagttagta aaaatcctgc tggtggatga aaggagtgtt gctcatgtta ctgaggcaga
     6721 tctgttccac accattgaaa ccctcatgag gattaatgct cattccagag gcaatgctcc
     6781 agaaggattg ccccagctga tggaagtagt gcgatccaac tatgaagcaa tgattgatcg
     6841 tgctcatgga ggcccaaact ttatgatgca ttctgggatc tctcaagcct cagagtatga
     6901 tgaccctcca ggcctgaggg agaaggcaga gtatcttctg agggaatggg tgaatctcta
     6961 ccattcagca gcagctggcc gcgacagtac caaagctttc tctgcatttg ttggacagat
     7021 gcaccagcaa ggaatactga agaccgatga tctcataaca aggttctttc gtctgtgtac
     7081 tgaaatgtgt gttgaaatca gttaccgtgc tcaggctgag cagcagcaca atcctgctgc
     7141 caatcccacc atgatccgag ccaagtgcta tcacaacctg gatgcctttg ttcgactcat
     7201 tgcactgctc gtgaaacact caggggaggc caccaacact gtcacaaaga ttaatctgct
     7261 gaacaaggtc cttggtatag tagtgggagt tctccttcag gatcatgatg ttcgtcagag
     7321 tgaatttcag caacttccct accatcgaat ttttatcatg cttctcttgg aactcaatgc
     7381 acctgagcat gtgttggaaa ccattaattt ccagacactt acagctttct gcaatacatt
     7441 ccacatcttg aggcctacca aagctcctgg ctttgtatat gcctggcttg aactgatttc
     7501 ccatcggata tttattgcaa gaatgctggc acatacgcca cagcagaagg ggtggcctat
     7561 gtatgcacag ctactgattg atttattcaa atatttagcg cctttcctta gaaatgtgga
     7621 actcaccaaa cctatgcaaa tcctctacaa gggcacttta agagtgctgc tggttctttt
     7681 gcatgatttc ccagagttcc tttgtgatta ccattatggg ttctgtgatg tgatcccacc
     7741 taattgtatc cagttaagaa atttgatcct gagtgccttt ccaagaaaca tgaggctccc
     7801 cgacccattc actcctaatc taaaggtgga catgttgagt gaaattaaca ttgctccccg
     7861 gattctcacc aatttcactg gagtaatgcc acctcagttc aaaaaggatt tggattccta
     7921 tcttaaaact cgatcaccag tcactttcct gtctgatctg cgcagcaacc tacaggtatc
     7981 caatgaacct gggaatcgct acaacctcca gctcatcaat gcactggtgc tctatgtcgg
     8041 gactcaggcc attgcgcaca tccacaacaa gggcagcaca ccttcaatga gcaccatcac
     8101 tcactcagca cacatggata tcttccagaa tttggctgtg gacttggaca ctgagggtcg
     8161 ctatctcttt ttgaatgcaa ttgcaaatca gctccggtac ccaaatagcc acactcacta
     8221 cttcagttgc accatgctgt acctttttgc agaggccaat acggaagcca tccaagaaca
     8281 gatcacaaga gttctcttgg aacggttgat tgtaaatagg ccacatcctt ggggtcttct
     8341 tattaccttc attgagctga ttaaaaaccc agcgtttaag ttctggaacc atgaatttgt
     8401 acactgtgcc ccagaaatcg aaaagttatt ccagtcggtc gcacagtgct gcatgggaca
     8461 gaagcaggcc cagcaagtaa tggaagggac aggtgccagt tagccgcggg cccgggatcc
     8521 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     8581 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     8641 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8761 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     8821 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     8881 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     8941 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     9001 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     9061 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     9121 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     9181 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     9241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     9301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     9361 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9481 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9541 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9661 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     9721 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     9781 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     9841 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     9901 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     9961 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10021 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10081 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10141 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    10201 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    10261 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    10321 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    10381 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    10441 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    10501 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    10561 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    10621 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10681 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
    10741 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
    10801 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
    10861 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
    10921 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
    10981 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
    11041 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    11101 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    11161 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    11221 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    11281 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    11341 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    11401 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    11461 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    11521 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11581 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11641 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11701 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11761 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    11821 cctgattctg tggataaccg tattaccgcc atgcat
//
