LOCUS       sequence-290366-        7155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmRUMP-del58-131.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7155)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmRUMP-del58-131_X
FEATURES             Location/Qualifiers
     source          1..7155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4440..4481
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4491..4508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4531..4548)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4581..4715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4605..4624)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4659..4678
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5337..5925)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5417..5436)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6096..6887,6888..6956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6719..6738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6957..7061)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7129..7147
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatga gcatggacgc tagtaactcg gtggagagcc gcgagaagga
     2761 gcgcgaccgt cgtggacgcg gagcacgtgg ctcccgtttc acggacgctg atggcaatgg
     2821 aaacggcgct ggtagtcagg gaggtggcgt cgctgctcgc gaccgtagcc gggaacgtcg
     2881 caattgcgag gatcacggcg agcagcgcga tcaatacgga cgcattgtgc gagatggtgg
     2941 tggtggtgga ggcggcggtg gcggcgtaca aggaggcaat ggtggcaaca atggaggagg
     3001 tggcggcggt ggccgtgacc acatggatga ccgcgatcgg ggtttctccc ggcgagacga
     3061 cgacagacta tctgggcgta ataattttaa catgatgtca aatgattata ataattcgtc
     3121 gaattacaat ttgtatgggc tttctgcttc gtttttggag agtcttggca taagtggacc
     3181 tttgcataat aaggtttttg ttgctaatct ggactacaag gtcgacaaca agaagctcaa
     3241 gcaggtcttc aagctggccg gcaaggtgca gagcgtagac ctttcactgg acaaggaggg
     3301 caacagccgc ggttttgctg tgatcgagta cgaccatccg gtggaggctg ttcaggccat
     3361 ttccatgctg gatcgtcaga tgctattcga ccgtcgtatg accgtgcgcc tggaccgaat
     3421 tccggataag aatgaaggca taaagctgcc cgagggcttg ggaggtgtgg gcatcggcct
     3481 gggacccaac ggcgagccac tcagggacgt ggctcacaac cttcccaatg gtggtcagag
     3541 ccagggccag ttgcttggca acgctcagca gggatctcag ctgggatcgg tgggtagcca
     3601 accaaacagc agcgctgtta gcaacgccac gacaaacctg cttaacaacc tgaccggcgt
     3661 tatgtttggc aatcatgcgg cagtgcagcc atcgcctgta gctccggtgc aaaagcctag
     3721 cctaggaaac aataccggtt ccggcggatt gaacctaaac aatctgaatc ccagcatttt
     3781 ggctgccgtg gtcggcaatc ttggtaacca gggagggaat ttaagcaatc ccttgctgtc
     3841 ctcttcgctg agcaacctgg gtctcaatct gggcaactcc ggcaatgatg acaacctgcc
     3901 gccaagcaat gtgggtcttt caaacaacta cagcagcggc ggcaccggtg gtggcaatag
     3961 ctatagctct ggaaacaact acagcggcgg tggcggctcc agcaatttgg gatacaatgc
     4021 ttacagcagc tctggtggaa tgggcggcgg caacggagga gttggtgttg atggcaacga
     4081 ttacaacact ggcaatccat tggatgtcta tggcggcggc agcaacgtgg gcaattccaa
     4141 cgttggctcg gccaatgctg tcggtgcttc tcgaaagtcg gacacgatca ttatcaagaa
     4201 cgttccaata acctgcactt ggcaaacact gcgcgacaag ttccgagaga tcggggacgt
     4261 taagtttgcg gaaatccgtg gcaatgatgt gggcgttgtg cgtttcttta aggagcgcga
     4321 tgccgagcta gccattgcgc ttatggatgg ctcccgtctg gatgggcgca acattaaagt
     4381 aacatacttt taagtcaggc aattcagttc actcgagtct agagggcccg cggttcgaag
     4441 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     4501 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc
     4561 gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa
     4621 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     4681 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     4741 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4801 gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc
     4861 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     4921 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     4981 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     5041 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     5101 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     5161 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     5221 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     5281 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5341 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5401 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5461 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5521 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5581 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5641 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5701 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5761 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5821 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     5881 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     5941 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6001 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6061 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6121 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6181 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6241 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6301 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6361 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     6421 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6481 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6541 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6601 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6661 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     6721 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6781 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6841 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     6901 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6961 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7021 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7081 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7141 acgaggccct ttcgt
//
