LOCUS       sequence-29041-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Jodoin J, Misiewicz M, Makhijani P, Giannopoulos PN, Hammond J, 
            Goodyer CG, LeBlanc AC
  TITLE     Loss of anti-Bax function in gerstmann-Straussler-Scheinker 
            syndrome-associated prion protein mutants.
  JOURNAL   PLoS One. 2009  . 4(8):e6647.
  PUBMED    19680558
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2009  . 4(8):e6647."
COMMENT     Sequence Label: pBud-EGFP
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             135..164
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             180..197
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    228..349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(239..258)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     293..312
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagcgagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgcatt cctgcaggtc gacatcgatc ttaagcagta
      121 cttctagagg atccgaacaa aaactcatct cagaagagga tctgaatatg cataccggtc
      181 atcatcacca tcaccattga gtttgatccc cgggaattca gacatgataa gatacattga
      241 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      301 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga
      361 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
      421 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc
      481 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
      541 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
      601 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
      661 aggccagggt gttgtcccgc accacctggt cctggaccgc gctgatgaac agg
//
