LOCUS       sequence-290415-        9269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial Expression of CtNot2-CtNot33.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Raisch T, Sandmeir F, Weichenrieder O, Valkov E, Izaurralde E
  TITLE     Structural and biochemical analysis of a NOT1 MIF4G-like domain of 
            the CCR4-NOT complex.
  JOURNAL   J Struct Biol. 2018 Dec;204(3):388-395. doi: 
            10.1016/j.jsb.2018.10.009. Epub 2018 Oct 24.
  PUBMED    30367941
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jsb.2018.10.009"; journalName: "J Struct Biol"; date: 
            "2018-12"; volume: "204"; issue: "3"; pages: "388-395"
COMMENT     Sequence Label: pnEA-NpB-CtNot2-NpS-CtNot3_X
FEATURES             Location/Qualifiers
     source          1..9269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(18..36)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        104..208
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(209..277,278..1069)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(427..446)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1240..1828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1729..1748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1982..1999
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2014..2156
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2082..2101)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2242..2264
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2258..2449)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3761..4843)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4805..4824
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4844..4921)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5127..5146
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5230..5249
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5230..5248
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5249..5273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5288..5310
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5518..5541
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     RBS             6688..6710
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6729..6752
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6774..6797
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6798..6818
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      9010..9057
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(9232..9237,9238..9254,9255..9260))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
       61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      181 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
      481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
      541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
      601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
      661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
      721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
      781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
      841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
      901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
      961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
     2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
     2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
     2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
     2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
     2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
     2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
     2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
     2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
     2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
     2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
     2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
     2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
     2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
     3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
     3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
     3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
     3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
     3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
     3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
     3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
     3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
     3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
     3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
     3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
     3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     3721 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     3781 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3841 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc
     3901 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
     3961 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat
     4021 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
     4081 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
     4141 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
     4201 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
     4261 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
     4321 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg
     4381 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
     4441 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
     4501 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
     4561 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
     4621 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
     4681 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
     4741 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
     4801 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     4861 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     4921 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     4981 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     5041 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     5101 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     5161 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     5221 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tccccactag
     5281 taataatttt gtttaacttt aagaaggaga tataccatgg gcagcagcat gcagtataaa
     5341 ctgattctga atggcaaaac cctgaaaggt gaaaccacca ccgaagcagt tgatgcagcc
     5401 accgcagaaa aagtttttaa acagtatgcc aatgataacg gcgttgatgg tgaatggacc
     5461 tatgatgatg caaccaaaac ctataccgtt accgaaagca gcggtaccgg cagcggcctg
     5521 gaagttctgt tccaggggcc ccatatgctc gagatgtggg gtgcacaggg tccgcgtagc
     5581 ctgggtgcag cagcagttca tcgtggtgca ccgacaccgg ttagcgcaca gcaggcccag
     5641 caggatgacc tgtttagcag ccgtctgacc ggtgcccagg caggtatgcg ttttggtaat
     5701 cagggtagcg ttgcacaggc acatggtcag gttccggttc cggatgattt tccgcctctg
     5761 aatcgtgcaa ccaatggtga tgttggtggt caagaacgtg gtgttatgag taatattgca
     5821 tttggtgcac agaatgttgc accgggtagc ggtgttcaga atcgtgccaa taatggtctg
     5881 ctgaatgcac tgagcgcaac cagccgtgcc ggtgataccc gtccgcaggc agccattgca
     5941 cgtcctcagg atgcacgtag cccgaccggt aatgatgaag caccgcagaa agatggtatg
     6001 gatgcaatta gcggtaaaaa tccgcatggt gccattggca atgatgcacc gagcggtaaa
     6061 ggtaaagaag atgaaaaacc gacccgtcct ccggcacagg atccgctgga aggtatggca
     6121 ccggcagata aatggggtat taaaggtctg cgtctgctga tgaataactt cccggattat
     6181 aatgcactgg cctgtggtat tgatccgacc accctgaata tggatctgcg tagcagcgaa
     6241 ccgctgagca ccaaagttta tagcctgttt gatgatgttc ctccgcgtgc accggttccg
     6301 aaatttgaac tgccggaatg ttatcaggtt aaaaatgttc agccgatcga agccaaaatc
     6361 agcagcttta atgaagaaac cctgatgtgg atcttttata gctgtccgcg tgatattaaa
     6421 cagcagatgg cagcgattga actgaataat cgtaattggc gctggcacaa aaaactgcag
     6481 ctgtggctga ccaaagatga tctgatggca ccgcaggttc tgggtccgaa tcatgaacgt
     6541 ggttattatg ttgtttggga taccgtgaat tggcgtaaag aacgtcgtga actgaccctg
     6601 cattatgcag atctggatac caatccgggt acaccgctgc agagcattgc agcataacaa
     6661 ttgggatcct aatagtctag taataatttt gtttaacttt aagaaggaga tataccatgg
     6721 gcagcagctg gagccacccg cagttcgaaa aaagcagcgg taccggcagc ggcctggaag
     6781 ttctgttcca ggggcccgaa aacctttact tccagggcca tatgctcgag atggcagcac
     6841 gtaaactgca gcaagaggtt gataaatgct tcaaaaaagt tgccgaaggc gtgagcgaat
     6901 ttgaagcaat ctatgaaaaa atcgagcaga gcaccaatca ggcccagaaa gaaaaactgg
     6961 aagatcagct gaaacgcgaa atcaaaaaac tgcaacgtct gcgcgatcag attaaaacct
     7021 gggcagcaag caatgacatc aaagataaag caccgctgct ggaacagcgt cgtctgattg
     7081 aaacccagat ggaaaaattc aaagccgtgg aaaaagccat gaaaaccaaa gcctatagca
     7141 aagaaggtct gagcgcagca gcaaaactgg acccgaaaga acaggcaaaa attgaagcaa
     7201 gcgaattcct gagcaatatg gttgatgaac tggaacaaca aatcgaaacc ctggaagcag
     7261 aaagcgaaag tctgcaggca accatgaaaa aaggtaaagg tcatgcagca aaagcagaac
     7321 gtgttgcaga aattgaacgt attatcgatc gccataaatg gcatcagggt aaactggaac
     7381 tgattcgtcg tagcctggaa aatggtggtg ttgatgcaga acaggttaat gatctggaag
     7441 aaagcatcaa atattacgtg tccgatggta tgaacggcga ttttatggaa gatgaaggca
     7501 tgtatgatga tctgagcctg caggatgaag aggatgccta tggcatgaat caggataatg
     7561 ataaaaccag cagccaggat aatcagagca ttcagtcaag caaagaaaaa aaagaagagg
     7621 aacaagaagc cgagcagccg gaaaaaccga gcaaaccggc accggcactg agcggtaaac
     7681 agcgtgcagc agttgaagca gcaaccaatg caggtcgtcg tccgagcaac cagctgaaaa
     7741 gtccgctgcc gaccctggca accgtgcata ccaccagtag cagcaatctg agcaatggca
     7801 ccagcggtcc gcagatgaaa ccggcagcag ttccgagccg tctggcaggc gaaggcctga
     7861 aatatgcaag cgcagcggca gcagcagccg ctaatgaaaa aaacaatgtt ggtattgctc
     7921 cgctgcctcc gcctccggca aatgcacctg gtggtccggg tccgcagggt cgtattagca
     7981 ccaccaatag tccgcgtgtt ccgagcagtc agcctgccgg tcaggataaa gcaagcaccg
     8041 caggtagcag cagcaccgtt acaccggcaa aagccggtgc acgtagcaaa gcagccgcac
     8101 agccaccggc acaggcagaa gccgaaagca gcaaagccac agcacagacc aatggtgcca
     8161 gcaatgttaa accgagccaa gaagctgaag aagaagaacc gatttatcat ctgcctccgt
     8221 cactgcagga tctggttgat agctatgaaa ttagcaaaaa acgtgccgca gcactgagca
     8281 gcccgagcac ccagcgtatg caggcagtta gcaatgcaag caaaccgagc gcactggatg
     8341 gtgaaccgcc tcgtagctat cagccggaag ttcgttttta tagccatacc cgttatccgc
     8401 aagaaccgct gcctattttt gatgatccgc gtctgtatag ccgtattgat agcgataccc
     8461 tgttttacgt gttctattac aaacagggca cctatcagca gtatctggca gcccgtgcac
     8521 tgaaagatca gagttggcgt tttcataaac agtatcagac ctggtttcag cgtcatgaag
     8581 aacctaaaca aatcaccgaa gaatttgaga aaggcaccta ccgctttttc gattatgaaa
     8641 gcacctggat gaatcgtcgt aaagccgatt ttgaattcca ctataaatac ctggaagatg
     8701 aactggtgca tctgaatacc ggtcagagcg ttatggcact gcgttatcgt cgcctgagca
     8761 gtaaaattcc gcctgttaat tgggaagatc cgcctggtta tatggatctg gcaatgctgg
     8821 gtattaccag cagtcgtaat gatgaaacct cacgtgaatt tgatgcagtg tggtatcgta
     8881 atgaagccat gtgggataaa agtgatgcag cctaaggatc ctaatagtct agagctagcc
     8941 ctaggagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     9001 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     9061 aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc ataaccaagc
     9121 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
     9181 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct
     9241 tatcgatgat aagctgtcaa acatgagaa
//
