LOCUS       sequence-290479-        9773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del130-133G-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN2-del130-133G-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7058..7099
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7109..7126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7149..7166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7199..7333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7223..7242)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7277..7296
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7955..8543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8035..8054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8714..9505,9506..9574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9337..9356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9575..9679)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9747..9765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat ggactatgtc tactgtggaa cggatccgat
     3361 tggagctagc gaggatatcc tgtccgtgta cgatgccggc agtgcccccg gcaatggtca
     3421 tttcagcccg tcctttaacg gctttaatat tggaacgacg gaccccgagt acgtcgaact
     3481 ggtcccggtc ctggctgatg gaggtgaaca ttttggagtg agctccgtcg cctttgatga
     3541 ttatgaggaa ttgttgtgga tgggaaatca aggaggacat gtgaccagct attataccaa
     3601 tagcatgcaa aaatatacca gcttccaagt gcatgctacg gatatcgtcc gtgatattag
     3661 cacgttggat agcggcgtct tggccctgac ccaaacgagc ttgcgccacc aaattggccc
     3721 gaaattcacg tttaagagca ataatatgaa agaaatggtc tccatgttgc aactgtcgcc
     3781 ccaccgcttg gtgatggctg gcttgcaaga tgaactgatt gattttgatt tgcgtaccct
     3841 gaaagagacg cgtattgaac acgtcggcgc tggaggctgt accgtcttgc gtaaaaactc
     3901 gcgctacttg ttcgctggag atcaattggg caccgttacc ctgcgtgatt tgaattcctt
     3961 gtccgtgcaa cataccatta aaacccatac gaatattctg agcgattttt ccgtgcaagg
     4021 caacttgctg attagttgtg gctacagcgg aaggcaaaat aacctggcca tcgaccgctt
     4081 cctaatggtc tacgatctgc gcatgctgcg cctgatcgcg cccattcaag tgatgattga
     4141 tccgcagatg ttgaagttcc tgccatcgct gacatcccaa ctggccgtcg tgtccagcta
     4201 cggccaggtt cagctggtgg acactgtgga gctcagcgag ccgcgagtat ccatgtacca
     4261 gatcaacacc aacggcagcc agtgcctcag cttcgacatt agctcctctt cacaggccat
     4321 ggcatttggg gaccagtcgg gccacatcaa catgattgcc gctgtacaaa cgcctcagcc
     4381 gcagttcaat ctctactcgc gaagcacaga gtttgcggac gtggtgccac agctgccgat
     4441 ggtctccatt acggacacca actttccgct atcgtcggtc atgctaccgc acttgacgac
     4501 gggcacacaa tggttctcag attggccgga ggaactgctg cgctaccgct accacaggcc
     4561 caagaccatt gatcccgaag tgctgagcaa catgaaaatg cagggaccga ttggatactc
     4621 gccaaaccca cgcaccgccc gtcgtaatca aattccgtac gtgattgagc agggcggcgt
     4681 gtgcagtccg aacggaaatg gaactgcggc ggcgaccaag gcggagaacg gtgtgaaaat
     4741 cattccacgg cggtatcgca aagtcgagct gaagtacacg aagctgggca cccaggactt
     4801 tgactttgac cagcataatc aaacatgttt cgcggggctg gaggcaacgc taccgaactc
     4861 ctactgcaat gctatgctac agattttgta tttcaccgac gcgcttcgcg tgaagctgct
     4921 ggagcattcg tgcatcaagg agttttgcct gtcctgcgaa ctagggttcc tcttcaacat
     4981 gctggataaa agtaccgctt cttcaccttg ccaggccagc aatttcctgc gatctttccg
     5041 cactgtgcca gaggcctcgg cactgggatt gattctcacc gaccgctcat cgaacgtcaa
     5101 tctaatatcg ctgatacaga actggaaccg attcattttg catcaaatgc attacgaaat
     5161 attcgattct agcaagaacg catccaccta cagcggatct gtgcaaacat caacgaacgc
     5221 ggaaaacgcg ggctcttcag aaacaagtgg ctcctcggac ctttacgatt ccataagtga
     5281 tgagaactcc aaggaggacg atcgggaacg cagtaagatc aacgcagaaa ctgacatcag
     5341 taagatcttt gggaccaagc aaatctgtat aaaccgctgc atcaaatgcc aagaagagaa
     5401 gataaaggaa agcattctac ttgcctgcaa tttgtcgtat cccaaccaca tcaaagatag
     5461 tgatcagtat ttcaactttg gcaccatttt gaagcgatct ctcagctccg agaagagtat
     5521 tcaggccttc tgcgagagat gtaagaagtt ttcgccaacc aatcagagcg tgaaggtgac
     5581 ttcgcttccg caaattctgt ccattaactg tggcctcaac aacgaaaaag acattacctt
     5641 cctgaagcgc cagctcaatc gctgcagcga aaagacgact gttgatgcgg ccgcttcctt
     5701 gagcacaagc aagccgtgtc gatatggagc caactgttcg cgaagtgact gccatttcat
     5761 gcatccggat cgaaaatctc catcgcacac aagccaaccg aatgctgtca acaattctcc
     5821 caatggacgc caaaagtcct ggtttcccct tacatttact atgggcatca atgaccaagg
     5881 tgaggtgcag gtgcaaactc aatcagatgc aagcagtggc aaatcggaac aggaggagga
     5941 aacggaaaag ccaccaacaa agggcttgga caacaatcgg atgtacgcgc tgcatgccgt
     6001 ggtgtgccaa gtcgatgatg gtacccaaaa gaacttggta tcgttgataa atgtgcagcg
     6061 gccataccac accatgaaac tggctgaatc tgcagatgac cctcagagtc aatggtatat
     6121 ttttaatgat tttagcatat cgcccgtttc gccacaagaa tcggtatggt tcacattgga
     6181 ttggaaagtt ccttgcatac tcttctacag acacgtagag gatgactctg agtctgctag
     6241 tactactagc tcgaccgtca cggaaagtga agaaaccatt ccatcagaga gcagcagcgg
     6301 ctcgccaaca aatctatcca acccttttct agaggaaatt gtgagtccta tgctaggcaa
     6361 cttaagtgca gacgccacgc tgcagcccct gcagtcagat gaaatgccgc agtcgggtga
     6421 tctggtcgca atggatgccg agttcgttac cctaaatcca gaggaaaatg agattcgccc
     6481 ggatggcaaa acggcgacca ttaagccatg ccacatgagt gtggcccgga tttcatgtat
     6541 acgaggacaa ggcccggctg aaggagttcc attcatggac gattacatat ccacccagga
     6601 gaaggtcgtg gactacctaa ctcagttctc gggaatcaag cctggcgact tggacgctaa
     6661 cttcagcaaa aagcgactga ctgccttgaa gtactcatac cagaaattaa aatacctcgt
     6721 tgacgtagga gtaatatttg tcggacatgg tctgaagaac gactttaggg tcataaacat
     6781 ctatgtaccg tcggagcaga taatagatac ggtgcacctg tttcacatgc cacatcatcg
     6841 catggtttcc ctaagatttc tagcctggca tttcttgggt actaagattc agtccgagac
     6901 gcacgactcc atcgaagatg cacggaccac tctgcagctc tacaagcact atttgaagct
     6961 gcaggaggag aagaagtttg ccaacgctct caagaatctt tacgaacgcg gaaagcagct
     7021 gcagtggaag gttccagagg actgaccgcg gttcgaaggt aagcctatcc ctaaccctct
     7081 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     7141 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
     7201 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7261 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7321 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     7381 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     7441 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     7501 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     7561 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     7621 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     7681 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     7741 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     7801 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     7861 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg
     7921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     8161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9001 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     9061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9301 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     9361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9721 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgt
//
