LOCUS       sequence-290483-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del358-467-T97RH102G-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN2-del358-467-T97RH102G-dsRNA...
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6737..6778
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6788..6805
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6828..6845)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6878..7012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6902..6921)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6956..6975
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7634..8222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7714..7733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8393..9184,9185..9253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9016..9035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9254..9358)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9426..9444
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat ggactatgtc tactgtggaa cggatccgat
     3361 tggagctagc gaggatatcc tgtccgtgta cgatgccggc agtgcccccg gcaatggtca
     3421 tttcagcccg tcctttaacg gctttaatat tggaacgacg gaccccgagt acgtcgaact
     3481 ggtcccggtc ctggctgatg gaggtgaaca ttttggagtg agctccgtcg cctttgatga
     3541 ttatgaggaa ttgttgtgga tgggaaatca aggaggacat gtgaccagct attataccaa
     3601 tagcatgcaa aaatatcgca gcttccaagt gggtgctacg gatatcgtcc gtgatattag
     3661 cacgttggat agcggcgtct tggccctgac ccaaacgagc ttgcgccacc aaattcgtcg
     3721 cggcttgccg aaattcacgt ttaagagcaa taatatgaaa gaaatggtct ccatgttgca
     3781 actgtcgccc caccgcttgg tgatggctgg cttgcaagat gaactgattg attttgattt
     3841 gcgtaccctg aaagagacgc gtattgaaca cgtcggcgct ggaggctgta ccgtcttgcg
     3901 taaaaactcg cgctacttgt tcgctggaga tcaattgggc accgttaccc tgcgtgattt
     3961 gaattccttg tccgtgcaac ataccattaa aacccatacg aatattctga gcgatttttc
     4021 cgtgcaaggc aacttgctga ttagttgtgg ctacagcgga aggcaaaata acctggccat
     4081 cgaccgcttc ctaatggtct acgatctgcg catgctgcgc ctgatcgcgc ccattcaagt
     4141 gatgattgat ccgcagatgt tgaagttcct gccatcgctg acatcccaac tggccgtcgt
     4201 gtccagctac ggccaggttc agctggtgga cactgtggag ctcagcgagc cgcgagtatc
     4261 catgtaccag atcaacacca acggcagcca gtgcctcagc ttcgacatta gctcctcttc
     4321 acaggccatg gcatttgggg accagtcggg ccacatcaac atgattgccg ctgtacaaac
     4381 gcctcagccg cagttcaatg cggagaacgg tgtgaaaatc attccacggc ggtatcgcaa
     4441 agtcgagctg aagtacacga agctgggcac ccaggacttt gactttgacc agcataatca
     4501 aacatgtttc gcggggctgg aggcaacgct accgaactcc tactgcaatg ctatgctaca
     4561 gattttgtat ttcaccgacg cgcttcgcgt gaagctgctg gagcattcgt gcatcaagga
     4621 gttttgcctg tcctgcgaac tagggttcct cttcaacatg ctggataaaa gtaccgcttc
     4681 ttcaccttgc caggccagca atttcctgcg atctttccgc actgtgccag aggcctcggc
     4741 actgggattg attctcaccg accgctcatc gaacgtcaat ctaatatcgc tgatacagaa
     4801 ctggaaccga ttcattttgc atcaaatgca ttacgaaata ttcgattcta gcaagaacgc
     4861 atccacctac agcggatctg tgcaaacatc aacgaacgcg gaaaacgcgg gctcttcaga
     4921 aacaagtggc tcctcggacc tttacgattc cataagtgat gagaactcca aggaggacga
     4981 tcgggaacgc agtaagatca acgcagaaac tgacatcagt aagatctttg ggaccaagca
     5041 aatctgtata aaccgctgca tcaaatgcca agaagagaag ataaaggaaa gcattctact
     5101 tgcctgcaat ttgtcgtatc ccaaccacat caaagatagt gatcagtatt tcaactttgg
     5161 caccattttg aagcgatctc tcagctccga gaagagtatt caggccttct gcgagagatg
     5221 taagaagttt tcgccaacca atcagagcgt gaaggtgact tcgcttccgc aaattctgtc
     5281 cattaactgt ggcctcaaca acgaaaaaga cattaccttc ctgaagcgcc agctcaatcg
     5341 ctgcagcgaa aagacgactg ttgatgcggc cgcttccttg agcacaagca agccgtgtcg
     5401 atatggagcc aactgttcgc gaagtgactg ccatttcatg catccggatc gaaaatctcc
     5461 atcgcacaca agccaaccga atgctgtcaa caattctccc aatggacgcc aaaagtcctg
     5521 gtttcccctt acatttacta tgggcatcaa tgaccaaggt gaggtgcagg tgcaaactca
     5581 atcagatgca agcagtggca aatcggaaca ggaggaggaa acggaaaagc caccaacaaa
     5641 gggcttggac aacaatcgga tgtacgcgct gcatgccgtg gtgtgccaag tcgatgatgg
     5701 tacccaaaag aacttggtat cgttgataaa tgtgcagcgg ccataccaca ccatgaaact
     5761 ggctgaatct gcagatgacc ctcagagtca atggtatatt tttaatgatt ttagcatatc
     5821 gcccgtttcg ccacaagaat cggtatggtt cacattggat tggaaagttc cttgcatact
     5881 cttctacaga cacgtagagg atgactctga gtctgctagt actactagct cgaccgtcac
     5941 ggaaagtgaa gaaaccattc catcagagag cagcagcggc tcgccaacaa atctatccaa
     6001 cccttttcta gaggaaattg tgagtcctat gctaggcaac ttaagtgcag acgccacgct
     6061 gcagcccctg cagtcagatg aaatgccgca gtcgggtgat ctggtcgcaa tggatgccga
     6121 gttcgttacc ctaaatccag aggaaaatga gattcgcccg gatggcaaaa cggcgaccat
     6181 taagccatgc cacatgagtg tggcccggat ttcatgtata cgaggacaag gcccggctga
     6241 aggagttcca ttcatggacg attacatatc cacccaggag aaggtcgtgg actacctaac
     6301 tcagttctcg ggaatcaagc ctggcgactt ggacgctaac ttcagcaaaa agcgactgac
     6361 tgccttgaag tactcatacc agaaattaaa atacctcgtt gacgtaggag taatatttgt
     6421 cggacatggt ctgaagaacg actttagggt cataaacatc tatgtaccgt cggagcagat
     6481 aatagatacg gtgcacctgt ttcacatgcc acatcatcgc atggtttccc taagatttct
     6541 agcctggcat ttcttgggta ctaagattca gtccgagacg cacgactcca tcgaagatgc
     6601 acggaccact ctgcagctct acaagcacta tttgaagctg caggaggaga agaagtttgc
     6661 caacgctctc aagaatcttt acgaacgcgg aaagcagctg cagtggaagg ttccagagga
     6721 ctgaccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
     6781 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
     6841 ttctaaggcc tgagctcgct gatcagcctc gatcgaggat ccagacatga taagatacat
     6901 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     6961 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     7021 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     7081 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag tcgaccgatg cccttgagag
     7141 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     7201 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt
     7261 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg
     7321 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg
     7381 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt
     7441 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg
     7501 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc
     7561 ttcaaggatc gctcgcggct cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg
     7621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7801 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     7861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8041 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8701 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9001 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9361 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9421 ataaaaatag gcgtatcacg aggccctttc gt
//
