LOCUS       sequence-290485-        9767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del486-493GSG-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN2-del486-493GSG-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7052..7093
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7103..7120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7143..7160)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7193..7327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7217..7236)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7271..7290
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7949..8537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8029..8048)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8708..9499,9500..9568))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9331..9350
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9569..9673)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9741..9759
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat ggactatgtc tactgtggaa cggatccgat
     3361 tggagctagc gaggatatcc tgtccgtgta cgatgccggc agtgcccccg gcaatggtca
     3421 tttcagcccg tcctttaacg gctttaatat tggaacgacg gaccccgagt acgtcgaact
     3481 ggtcccggtc ctggctgatg gaggtgaaca ttttggagtg agctccgtcg cctttgatga
     3541 ttatgaggaa ttgttgtgga tgggaaatca aggaggacat gtgaccagct attataccaa
     3601 tagcatgcaa aaatatacca gcttccaagt gcatgctacg gatatcgtcc gtgatattag
     3661 cacgttggat agcggcgtct tggccctgac ccaaacgagc ttgcgccacc aaattcgtcg
     3721 cggcttgccg aaattcacgt ttaagagcaa taatatgaaa gaaatggtct ccatgttgca
     3781 actgtcgccc caccgcttgg tgatggctgg cttgcaagat gaactgattg attttgattt
     3841 gcgtaccctg aaagagacgc gtattgaaca cgtcggcgct ggaggctgta ccgtcttgcg
     3901 taaaaactcg cgctacttgt tcgctggaga tcaattgggc accgttaccc tgcgtgattt
     3961 gaattccttg tccgtgcaac ataccattaa aacccatacg aatattctga gcgatttttc
     4021 cgtgcaaggc aacttgctga ttagttgtgg ctacagcgga aggcaaaata acctggccat
     4081 cgaccgcttc ctaatggtct acgatctgcg catgctgcgc ctgatcgcgc ccattcaagt
     4141 gatgattgat ccgcagatgt tgaagttcct gccatcgctg acatcccaac tggccgtcgt
     4201 gtccagctac ggccaggttc agctggtgga cactgtggag ctcagcgagc cgcgagtatc
     4261 catgtaccag atcaacacca acggcagcca gtgcctcagc ttcgacatta gctcctcttc
     4321 acaggccatg gcatttgggg accagtcggg ccacatcaac atgattgccg ctgtacaaac
     4381 gcctcagccg cagttcaatc tctactcgcg aagcacagag tttgcggacg tggtgccaca
     4441 gctgccgatg gtctccatta cggacaccaa ctttccgcta tcgtcggtca tgctaccgca
     4501 cttgacgacg ggcacacaat ggttctcaga ttggccggag gaactgctgc gctaccgcta
     4561 ccacaggccc aagaccattg atcccgaagt gctgagcaac atgaaaatgc agggaccgat
     4621 tggatactcg ccaaacccac gcaccgcccg tcgtaatcaa attccgtacg tgattgagca
     4681 gggcggcgtg tgcagtccga acggaaatgg aactgcggcg gcgaccaagg cggagaacgg
     4741 tgtgaaaatc attccacggc ggtatcgcaa agtcgagctg aagggcagcg gctttgactt
     4801 tgaccagcat aatcaaacat gtttcgcggg gctggaggca acgctaccga actcctactg
     4861 caatgctatg ctacagattt tgtatttcac cgacgcgctt cgcgtgaagc tgctggagca
     4921 ttcgtgcatc aaggagtttt gcctgtcctg cgaactaggg ttcctcttca acatgctgga
     4981 taaaagtacc gcttcttcac cttgccaggc cagcaatttc ctgcgatctt tccgcactgt
     5041 gccagaggcc tcggcactgg gattgattct caccgaccgc tcatcgaacg tcaatctaat
     5101 atcgctgata cagaactgga accgattcat tttgcatcaa atgcattacg aaatattcga
     5161 ttctagcaag aacgcatcca cctacagcgg atctgtgcaa acatcaacga acgcggaaaa
     5221 cgcgggctct tcagaaacaa gtggctcctc ggacctttac gattccataa gtgatgagaa
     5281 ctccaaggag gacgatcggg aacgcagtaa gatcaacgca gaaactgaca tcagtaagat
     5341 ctttgggacc aagcaaatct gtataaaccg ctgcatcaaa tgccaagaag agaagataaa
     5401 ggaaagcatt ctacttgcct gcaatttgtc gtatcccaac cacatcaaag atagtgatca
     5461 gtatttcaac tttggcacca ttttgaagcg atctctcagc tccgagaaga gtattcaggc
     5521 cttctgcgag agatgtaaga agttttcgcc aaccaatcag agcgtgaagg tgacttcgct
     5581 tccgcaaatt ctgtccatta actgtggcct caacaacgaa aaagacatta ccttcctgaa
     5641 gcgccagctc aatcgctgca gcgaaaagac gactgttgat gcggccgctt ccttgagcac
     5701 aagcaagccg tgtcgatatg gagccaactg ttcgcgaagt gactgccatt tcatgcatcc
     5761 ggatcgaaaa tctccatcgc acacaagcca accgaatgct gtcaacaatt ctcccaatgg
     5821 acgccaaaag tcctggtttc cccttacatt tactatgggc atcaatgacc aaggtgaggt
     5881 gcaggtgcaa actcaatcag atgcaagcag tggcaaatcg gaacaggagg aggaaacgga
     5941 aaagccacca acaaagggct tggacaacaa tcggatgtac gcgctgcatg ccgtggtgtg
     6001 ccaagtcgat gatggtaccc aaaagaactt ggtatcgttg ataaatgtgc agcggccata
     6061 ccacaccatg aaactggctg aatctgcaga tgaccctcag agtcaatggt atatttttaa
     6121 tgattttagc atatcgcccg tttcgccaca agaatcggta tggttcacat tggattggaa
     6181 agttccttgc atactcttct acagacacgt agaggatgac tctgagtctg ctagtactac
     6241 tagctcgacc gtcacggaaa gtgaagaaac cattccatca gagagcagca gcggctcgcc
     6301 aacaaatcta tccaaccctt ttctagagga aattgtgagt cctatgctag gcaacttaag
     6361 tgcagacgcc acgctgcagc ccctgcagtc agatgaaatg ccgcagtcgg gtgatctggt
     6421 cgcaatggat gccgagttcg ttaccctaaa tccagaggaa aatgagattc gcccggatgg
     6481 caaaacggcg accattaagc catgccacat gagtgtggcc cggatttcat gtatacgagg
     6541 acaaggcccg gctgaaggag ttccattcat ggacgattac atatccaccc aggagaaggt
     6601 cgtggactac ctaactcagt tctcgggaat caagcctggc gacttggacg ctaacttcag
     6661 caaaaagcga ctgactgcct tgaagtactc ataccagaaa ttaaaatacc tcgttgacgt
     6721 aggagtaata tttgtcggac atggtctgaa gaacgacttt agggtcataa acatctatgt
     6781 accgtcggag cagataatag atacggtgca cctgtttcac atgccacatc atcgcatggt
     6841 ttccctaaga tttctagcct ggcatttctt gggtactaag attcagtccg agacgcacga
     6901 ctccatcgaa gatgcacgga ccactctgca gctctacaag cactatttga agctgcagga
     6961 ggagaagaag tttgccaacg ctctcaagaa tctttacgaa cgcggaaagc agctgcagtg
     7021 gaaggttcca gaggactgac cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     7081 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     7141 agcctcgact gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga
     7201 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     7261 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     7321 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     7381 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac
     7441 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     7501 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     7561 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     7621 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
     7681 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     7741 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     7801 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     7861 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga
     7921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     8161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8521 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8641 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8701 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8761 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8821 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8881 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8941 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9001 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9061 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9121 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9181 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9241 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9301 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     9361 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9421 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9481 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9541 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9601 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9661 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9721 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
