LOCUS       sequence-290510-        9176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del130-133G-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del130-133G-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6461..6502
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6512..6529
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6552..6569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6602..6736
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6626..6645)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6680..6699
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7358..7946)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7438..7457)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8117..8908,8909..8977))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8740..8759
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8978..9082)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9150..9168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattgg
     3121 cccgaaattc acgtttaaga gcaataatat gaaagaaatg gtctccatgt tgcaactgtc
     3181 gccccaccgc ttggtgatgg ctggcttgca agatgaactg attgattttg atttgcgtac
     3241 cctgaaagag acgcgtattg aacacgtcgg cgctggaggc tgtaccgtct tgcgtaaaaa
     3301 ctcgcgctac ttgttcgctg gagatcaatt gggcaccgtt accctgcgtg atttgaattc
     3361 cttgtccgtg caacatacca ttaaaaccca tacgaatatt ctgagcgatt tttccgtgca
     3421 aggcaacttg ctgattagtt gtggctacag cggaaggcaa aataacctgg ccatcgaccg
     3481 cttcctaatg gtctacgatc tgcgcatgct gcgcctgatc gcgcccattc aagtgatgat
     3541 tgatccgcag atgttgaagt tcctgccatc gctgacatcc caactggccg tcgtgtccag
     3601 ctacggccag gttcagctgg tggacactgt ggagctcagc gagccgcgag tatccatgta
     3661 ccagatcaac accaacggca gccagtgcct cagcttcgac attagctcct cttcacaggc
     3721 catggcattt ggggaccagt cgggccacat caacatgatt gccgctgtac aaacgcctca
     3781 gccgcagttc aatctctact cgcgaagcac agagtttgcg gacgtggtgc cacagctgcc
     3841 gatggtctcc attacggaca ccaactttcc gctatcgtcg gtcatgctac cgcacttgac
     3901 gacgggcaca caatggttct cagattggcc ggaggaactg ctgcgctacc gctaccacag
     3961 gcccaagacc attgatcccg aagtgctgag caacatgaaa atgcagggac cgattggata
     4021 ctcgccaaac ccacgcaccg cccgtcgtaa tcaaattccg tacgtgattg agcagggcgg
     4081 cgtgtgcagt ccgaacggaa atggaactgc ggcggcgacc aaggcggaga acggtgtgaa
     4141 aatcattcca cggcggtatc gcaaagtcga gctgaagtac acgaagctgg gcacccagga
     4201 ctttgacttt gaccagcata atcaaacatg tttcgcgggg ctggaggcaa cgctaccgaa
     4261 ctcctactgc aatgctatgc tacagatttt gtatttcacc gacgcgcttc gcgtgaagct
     4321 gctggagcat tcgtgcatca aggagttttg cctgtcctgc gaactagggt tcctcttcaa
     4381 catgctggat aaaagtaccg cttcttcacc ttgccaggcc agcaatttcc tgcgatcttt
     4441 ccgcactgtg ccagaggcct cggcactggg attgattctc accgaccgct catcgaacgt
     4501 caatctaata tcgctgatac agaactggaa ccgattcatt ttgcatcaaa tgcattacga
     4561 aatattcgat tctagcaaga acgcatccac ctacagcgga tctgtgcaaa catcaacgaa
     4621 cgcggaaaac gcgggctctt cagaaacaag tggctcctcg gacctttacg attccataag
     4681 tgatgagaac tccaaggagg acgatcggga acgcagtaag atcaacgcag aaactgacat
     4741 cagtaagatc tttgggacca agcaaatctg tataaaccgc tgcatcaaat gccaagaaga
     4801 gaagataaag gaaagcattc tacttgcctg caatttgtcg tatcccaacc acatcaaaga
     4861 tagtgatcag tatttcaact ttggcaccat tttgaagcga tctctcagct ccgagaagag
     4921 tattcaggcc ttctgcgaga gatgtaagaa gttttcgcca accaatcaga gcgtgaaggt
     4981 gacttcgctt ccgcaaattc tgtccattaa ctgtggcctc aacaacgaaa aagacattac
     5041 cttcctgaag cgccagctca atcgctgcag cgaaaagacg actgttgatg cggccgcttc
     5101 cttgagcaca agcaagccgt gtcgatatgg agccaactgt tcgcgaagtg actgccattt
     5161 catgcatccg gatcgaaaat ctccatcgca cacaagccaa ccgaatgctg tcaacaattc
     5221 tcccaatgga cgccaaaagt cctggtttcc ccttacattt actatgggca tcaatgacca
     5281 aggtgaggtg caggtgcaaa ctcaatcaga tgcaagcagt ggcaaatcgg aacaggagga
     5341 ggaaacggaa aagccaccaa caaagggctt ggacaacaat cggatgtacg cgctgcatgc
     5401 cgtggtgtgc caagtcgatg atggtaccca aaagaacttg gtatcgttga taaatgtgca
     5461 gcggccatac cacaccatga aactggctga atctgcagat gaccctcaga gtcaatggta
     5521 tatttttaat gattttagca tatcgcccgt ttcgccacaa gaatcggtat ggttcacatt
     5581 ggattggaaa gttccttgca tactcttcta cagacacgta gaggatgact ctgagtctgc
     5641 tagtactact agctcgaccg tcacggaaag tgaagaaacc attccatcag agagcagcag
     5701 cggctcgcca acaaatctat ccaacccttt tctagaggaa attgtgagtc ctatgctagg
     5761 caacttaagt gcagacgcca cgctgcagcc cctgcagtca gatgaaatgc cgcagtcggg
     5821 tgatctggtc gcaatggatg ccgagttcgt taccctaaat ccagaggaaa atgagattcg
     5881 cccggatggc aaaacggcga ccattaagcc atgccacatg agtgtggccc ggatttcatg
     5941 tatacgagga caaggcccgg ctgaaggagt tccattcatg gacgattaca tatccaccca
     6001 ggagaaggtc gtggactacc taactcagtt ctcgggaatc aagcctggcg acttggacgc
     6061 taacttcagc aaaaagcgac tgactgcctt gaagtactca taccagaaat taaaatacct
     6121 cgttgacgta ggagtaatat ttgtcggaca tggtctgaag aacgacttta gggtcataaa
     6181 catctatgta ccgtcggagc agataataga tacggtgcac ctgtttcaca tgccacatca
     6241 tcgcatggtt tccctaagat ttctagcctg gcatttcttg ggtactaaga ttcagtccga
     6301 gacgcacgac tccatcgaag atgcacggac cactctgcag ctctacaagc actatttgaa
     6361 gctgcaggag gagaagaagt ttgccaacgc tctcaagaat ctttacgaac gcggaaagca
     6421 gctgcagtgg aaggttccag aggactgacc gcggttcgaa ggtaagccta tccctaaccc
     6481 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac
     6541 ccgctgatca gcctcgactg tgccttctaa ggcctgagct cgctgatcag cctcgatcga
     6601 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     6661 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     6721 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     6781 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga
     6841 tcagtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     6901 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     6961 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     7021 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     7081 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     7141 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     7201 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     7261 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agccagcaaa
     7321 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7381 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7441 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7501 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     7561 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7621 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7681 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7741 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7801 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7861 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7921 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7981 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8041 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8101 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8161 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8221 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8281 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8341 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8401 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     8461 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8521 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8581 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8641 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8701 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     8761 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8821 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8881 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8941 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9001 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9061 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     9121 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgt
//
