LOCUS       sequence-290512-        9173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del246-249-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9173)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del246-249-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..9173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6458..6499
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6509..6526
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6549..6566)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6599..6733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6623..6642)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6677..6696
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7355..7943)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7435..7454)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8114..8905,8906..8974))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8737..8756
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8975..9079)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9147..9165
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaagggcca tcgaccgctt
     3481 cctaatggtc tacgatctgc gcatgctgcg cctgatcgcg cccattcaag tgatgattga
     3541 tccgcagatg ttgaagttcc tgccatcgct gacatcccaa ctggccgtcg tgtccagcta
     3601 cggccaggtt cagctggtgg acactgtgga gctcagcgag ccgcgagtat ccatgtacca
     3661 gatcaacacc aacggcagcc agtgcctcag cttcgacatt agctcctctt cacaggccat
     3721 ggcatttggg gaccagtcgg gccacatcaa catgattgcc gctgtacaaa cgcctcagcc
     3781 gcagttcaat ctctactcgc gaagcacaga gtttgcggac gtggtgccac agctgccgat
     3841 ggtctccatt acggacacca actttccgct atcgtcggtc atgctaccgc acttgacgac
     3901 gggcacacaa tggttctcag attggccgga ggaactgctg cgctaccgct accacagacc
     3961 caagaccatt gatcccgaag tgctgagcaa catgaaaatg cagggaccga ttggatactc
     4021 gccaaaccca cgcaccgccc gtcgtaatca aattccgtac gtgattgagc agggcggcgt
     4081 gtgcagtccg aacggaaatg gaactgcggc ggcgaccaag gcggagaacg gtgtgaaaat
     4141 cattccacgg cggtatcgca aagtcgagct gaagtacacg aagctgggca cccaggactt
     4201 tgactttgac cagcataatc aaacatgttt cgcggggctg gaggcaacgc taccgaactc
     4261 ctactgcaat gctatgctac agattttgta tttcaccgac gcgcttcgcg tgaagctgct
     4321 ggagcattcg tgcatcaagg agttttgcct gtcctgcgaa ctagggttcc tcttcaacat
     4381 gctggataaa agtaccgctt cttcaccttg ccaggccagc aatttcctgc gatctttccg
     4441 cactgtgcca gaggcctcgg cactgggatt gattctcacc gaccgctcat cgaacgtcaa
     4501 tctaatatcg ctgatacaga actggaaccg attcattttg catcaaatgc attacgaaat
     4561 attcgattct agcaagaacg catccaccta cagcggatct gtgcaaacat caacgaacgc
     4621 ggaaaacgcg ggctcttcag aaacaagtgg ctcctcggac ctttacgatt ccataagtga
     4681 tgagaactcc aaggaggacg atcgggaacg cagtaagatc aacgcagaaa ctgacatcag
     4741 taagatcttt gggaccaagc aaatctgtat aaaccgctgc atcaaatgcc aagaagagaa
     4801 gataaaggaa agcattctac ttgcctgcaa tttgtcgtat cccaaccaca tcaaagatag
     4861 tgatcagtat ttcaactttg gcaccatttt gaagcgatct ctcagctccg agaagagtat
     4921 tcaggccttc tgcgagagat gtaagaagtt ttcgccaacc aatcagagcg tgaaggtgac
     4981 ttcgcttccg caaattctgt ccattaactg tggcctcaac aacgaaaaag acattacctt
     5041 cctgaagcgc cagctcaatc gctgcagcga aaagacgact gttgatgcgg ccgcttcctt
     5101 gagcacaagc aagccgtgtc gatatggagc caactgttcg cgaagtgact gccatttcat
     5161 gcatccggat cgaaaatctc catcgcacac aagccaaccg aatgctgtca acaattctcc
     5221 caatggacgc caaaagtcct ggtttcccct tacatttact atgggcatca atgaccaagg
     5281 tgaggtgcag gtgcaaactc aatcagatgc aagcagtggc aaatcggaac aggaggagga
     5341 aacggaaaag ccaccaacaa agggcttgga caacaatcgg atgtacgcgc tgcatgccgt
     5401 ggtgtgccaa gtcgatgatg gtacccaaaa gaacttggta tcgttgataa atgtgcagcg
     5461 gccataccac accatgaaac tggctgaatc tgcagatgac cctcagagtc aatggtatat
     5521 ttttaatgat tttagcatat cgcccgtttc gccacaagaa tcggtatggt tcacattgga
     5581 ttggaaagtt ccttgcatac tcttctacag acacgtagag gatgactctg agtctgctag
     5641 tactactagc tcgaccgtca cggaaagtga agaaaccatt ccatcagaga gcagcagcgg
     5701 ctcgccaaca aatctatcca acccttttct agaggaaatt gtgagtccta tgctaggcaa
     5761 cttaagtgca gacgccacgc tgcagcccct gcagtcagat gaaatgccgc agtcgggtga
     5821 tctggtcgca atggatgccg agttcgttac cctaaatcca gaggaaaatg agattcgccc
     5881 ggatggcaaa acggcgacca ttaagccatg ccacatgagt gtggcccgga tttcatgtat
     5941 acgaggacaa ggcccggctg aaggagttcc attcatggac gattacatat ccacccagga
     6001 gaaggtcgtg gactacctaa ctcagttctc gggaatcaag cctggcgact tggacgctaa
     6061 cttcagcaaa aagcgactga ctgccttgaa gtactcatac cagaaattaa aatacctcgt
     6121 tgacgtagga gtaatatttg tcggacatgg tctgaagaac gactttaggg tcataaacat
     6181 ctatgtaccg tcggagcaga taatagatac ggtgcacctg tttcacatgc cacatcatcg
     6241 catggtttcc ctaagatttc tagcctggca tttcttgggt actaagattc agtccgagac
     6301 gcacgactcc atcgaagatg cacggaccac tctgcagctc tacaagcact atttgaagct
     6361 gcaggaggag aagaagtttg ccaacgctct caagaatctt tacgaacgcg gaaagcagct
     6421 gcagtggaag gttccagagg actgaccgcg gttcgaaggt aagcctatcc ctaaccctct
     6481 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
     6541 ctgatcagcc tcgactgtgc cttctaaggc ctgagctcgc tgatcagcct cgatcgagga
     6601 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     6661 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     6721 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     6781 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
     6841 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     6901 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     6961 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     7021 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     7081 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     7141 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     7201 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     7261 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc cagcaaaagg
     7321 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7381 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7441 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7501 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     7561 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7621 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7681 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7741 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     7801 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7861 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7921 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7981 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8041 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8101 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8161 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8221 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8281 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8341 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8401 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     8461 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8521 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8581 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8641 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8701 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     8761 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8821 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8881 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8941 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9001 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9061 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9121 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgt
//
