LOCUS       sequence-290513-        8855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del358-467-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del358-467-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..8855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6140..6181
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6191..6208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6231..6248)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6281..6415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6305..6324)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6359..6378
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7037..7625)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7117..7136)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7796..8587,8588..8656))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8419..8438
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8657..8761)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8829..8847
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaaggcaaa ataacctggc
     3481 catcgaccgc ttcctaatgg tctacgatct gcgcatgctg cgcctgatcg cgcccattca
     3541 agtgatgatt gatccgcaga tgttgaagtt cctgccatcg ctgacatccc aactggccgt
     3601 cgtgtccagc tacggccagg ttcagctggt ggacactgtg gagctcagcg agccgcgagt
     3661 atccatgtac cagatcaaca ccaacggcag ccagtgcctc agcttcgaca ttagctcctc
     3721 ttcacaggcc atggcatttg gggaccagtc gggccacatc aacatgattg ccgctgtaca
     3781 aacgcctcag ccgcagttca atgcggagaa cggtgtgaaa atcattccac ggcggtatcg
     3841 caaagtcgag ctgaagtaca cgaagctggg cacccaggac tttgactttg accagcataa
     3901 tcaaacatgt ttcgcggggc tggaggcaac gctaccgaac tcctactgca atgctatgct
     3961 acagattttg tatttcaccg acgcgcttcg cgtgaagctg ctggagcatt cgtgcatcaa
     4021 ggagttttgc ctgtcctgcg aactagggtt cctcttcaac atgctggata aaagtaccgc
     4081 ttcttcacct tgccaggcca gcaatttcct gcgatctttc cgcactgtgc cagaggcctc
     4141 ggcactggga ttgattctca ccgaccgctc atcgaacgtc aatctaatat cgctgataca
     4201 gaactggaac cgattcattt tgcatcaaat gcattacgaa atattcgatt ctagcaagaa
     4261 cgcatccacc tacagcggat ctgtgcaaac atcaacgaac gcggaaaacg cgggctcttc
     4321 agaaacaagt ggctcctcgg acctttacga ttccataagt gatgagaact ccaaggagga
     4381 cgatcgggaa cgcagtaaga tcaacgcaga aactgacatc agtaagatct ttgggaccaa
     4441 gcaaatctgt ataaaccgct gcatcaaatg ccaagaagag aagataaagg aaagcattct
     4501 acttgcctgc aatttgtcgt atcccaacca catcaaagat agtgatcagt atttcaactt
     4561 tggcaccatt ttgaagcgat ctctcagctc cgagaagagt attcaggcct tctgcgagag
     4621 atgtaagaag ttttcgccaa ccaatcagag cgtgaaggtg acttcgcttc cgcaaattct
     4681 gtccattaac tgtggcctca acaacgaaaa agacattacc ttcctgaagc gccagctcaa
     4741 tcgctgcagc gaaaagacga ctgttgatgc ggccgcttcc ttgagcacaa gcaagccgtg
     4801 tcgatatgga gccaactgtt cgcgaagtga ctgccatttc atgcatccgg atcgaaaatc
     4861 tccatcgcac acaagccaac cgaatgctgt caacaattct cccaatggac gccaaaagtc
     4921 ctggtttccc cttacattta ctatgggcat caatgaccaa ggtgaggtgc aggtgcaaac
     4981 tcaatcagat gcaagcagtg gcaaatcgga acaggaggag gaaacggaaa agccaccaac
     5041 aaagggcttg gacaacaatc ggatgtacgc gctgcatgcc gtggtgtgcc aagtcgatga
     5101 tggtacccaa aagaacttgg tatcgttgat aaatgtgcag cggccatacc acaccatgaa
     5161 actggctgaa tctgcagatg accctcagag tcaatggtat atttttaatg attttagcat
     5221 atcgcccgtt tcgccacaag aatcggtatg gttcacattg gattggaaag ttccttgcat
     5281 actcttctac agacacgtag aggatgactc tgagtctgct agtactacta gctcgaccgt
     5341 cacggaaagt gaagaaacca ttccatcaga gagcagcagc ggctcgccaa caaatctatc
     5401 caaccctttt ctagaggaaa ttgtgagtcc tatgctaggc aacttaagtg cagacgccac
     5461 gctgcagccc ctgcagtcag atgaaatgcc gcagtcgggt gatctggtcg caatggatgc
     5521 cgagttcgtt accctaaatc cagaggaaaa tgagattcgc ccggatggca aaacggcgac
     5581 cattaagcca tgccacatga gtgtggcccg gatttcatgt atacgaggac aaggcccggc
     5641 tgaaggagtt ccattcatgg acgattacat atccacccag gagaaggtcg tggactacct
     5701 aactcagttc tcgggaatca agcctggcga cttggacgct aacttcagca aaaagcgact
     5761 gactgccttg aagtactcat accagaaatt aaaatacctc gttgacgtag gagtaatatt
     5821 tgtcggacat ggtctgaaga acgactttag ggtcataaac atctatgtac cgtcggagca
     5881 gataatagat acggtgcacc tgtttcacat gccacatcat cgcatggttt ccctaagatt
     5941 tctagcctgg catttcttgg gtactaagat tcagtccgag acgcacgact ccatcgaaga
     6001 tgcacggacc actctgcagc tctacaagca ctatttgaag ctgcaggagg agaagaagtt
     6061 tgccaacgct ctcaagaatc tttacgaacg cggaaagcag ctgcagtgga aggttccaga
     6121 ggactgaccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     6181 gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     6241 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     6301 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6361 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     6421 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     6481 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgaccg atgcccttga
     6541 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     6601 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     6661 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     6721 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg
     6781 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     6841 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     6901 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     6961 agcttcaagg atcgctcgcg gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg
     7021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7201 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     7261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7441 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7561 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7621 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7681 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7741 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7801 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7861 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7921 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7981 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8041 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8101 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8161 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8221 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8281 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8341 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8401 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8461 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8521 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8581 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8641 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8701 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8761 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8821 cctataaaaa taggcgtatc acgaggccct ttcgt
//
