LOCUS       sequence-290514-        7643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del468-981-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del468-981-dsRNAres_Y
FEATURES             Location/Qualifiers
     source          1..7643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4928..4969
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4979..4996
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5019..5036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5069..5203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5093..5112)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5147..5166
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5825..6413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5905..5924)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6584..7375,7376..7444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7207..7226
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7445..7549)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7617..7635
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaaggcaaa ataacctggc
     3481 catcgaccgc ttcctaatgg tctacgatct gcgcatgctg cgcctgatcg cgcccattca
     3541 agtgatgatt gatccgcaga tgttgaagtt cctgccatcg ctgacatccc aactggccgt
     3601 cgtgtccagc tacggccagg ttcagctggt ggacactgtg gagctcagcg agccgcgagt
     3661 atccatgtac cagatcaaca ccaacggcag ccagtgcctc agcttcgaca ttagctcctc
     3721 ttcacaggcc atggcatttg gggaccagtc gggccacatc aacatgattg ccgctgtaca
     3781 aacgcctcag ccgcagttca atctctactc gcgaagcaca gagtttgcgg acgtggtgcc
     3841 acagctgccg atggtctcca ttacggacac caactttccg ctatcgtcgg tcatgctacc
     3901 gcacttgacg acgggcacac aatggttctc agattggccg gaggaactgc tgcgctaccg
     3961 ctaccacagg cccaagacca ttgatcccga agtgctgagc aacatgaaaa tgcagggacc
     4021 gattggatac tcgccaaacc cacgcaccgc ccgtcgtaat caaattccgt acgtgattga
     4081 gcagggcggc gtgtgcagtc cgaacggaaa tggaactgcg gcggcgacca aggaaagtga
     4141 agaaaccatt ccatcagaga gcagcagcgg ctcgccaaca aatctatcca acccttttct
     4201 agaggaaatt gtgagtccta tgctaggcaa cttaagtgca gacgccacgc tgcagcccct
     4261 gcagtcagat gaaatgccgc agtcgggtga tctggtcgca atggatgccg agttcgttac
     4321 cctaaatcca gaggaaaatg agattcgccc ggatggcaaa acggcgacca ttaagccatg
     4381 ccacatgagt gtggcccgga tttcatgtat acgaggacaa ggcccggctg aaggagttcc
     4441 attcatggac gattacatat ccacccagga gaaggtcgtg gactacctaa ctcagttctc
     4501 gggaatcaag cctggcgact tggacgctaa cttcagcaaa aagcgactga ctgccttgaa
     4561 gtactcatac cagaaattaa aatacctcgt tgacgtagga gtaatatttg tcggacatgg
     4621 tctgaagaac gactttaggg tcataaacat ctatgtaccg tcggagcaga taatagatac
     4681 ggtgcacctg tttcacatgc cacatcatcg catggtttcc ctaagatttc tagcctggca
     4741 tttcttgggt actaagattc agtccgagac gcacgactcc atcgaagatg cacggaccac
     4801 tctgcagctc tacaagcact atttgaagct gcaggaggag aagaagtttg ccaacgctct
     4861 caagaatctt tacgaacgcg gaaagcagct gcagtggaag gttccagagg actgaccgcg
     4921 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     4981 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     5041 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     5101 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     5161 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     5221 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     5281 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     5341 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     5401 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     5461 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     5521 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     5581 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     5641 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     5701 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     5761 cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5821 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5881 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5941 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6001 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     6061 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6241 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     6301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     6901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7201 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7561 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     7621 ggcgtatcac gaggcccttt cgt
//
